Literature summary for 1.3.1.124 extracted from

Alphey, M.S.; Yu, W.; Byres, E.; Li, D.; Hunter, W.N.
Structure and reactivity of human mitochondrial 2,4-dienoyl-CoA reductase: enzyme-ligand interactions in a distinctive short-chain reductase active site (2005), J. Biol. Chem., 280, 3068-3077.

Search for more literature for 1.3.1.124

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), expression of selenomethionine enzyme in Escherichia coli strain B834(DE3)	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
wild-type and selenomethionine enzyme, 0.001 ml of protein and of reservoir solution, the latter containing 16% PEG 4000 w/v, 0.18 M ammonium sulfate, 80 mM sodium acetate, pH 4.6, 20% glycerol, addition of 30% ethylene glycol and 4 mM NADP+ for the binary complex of enzyme with NADP+, and 120 mM substrate for the ternary complex of enzyme with NADP+ and substrate trans-trans-2,4-dienoyl-CoA, X-ray diffraction structure determination and analysis at 2.1 A and 1.75 A resolution, respectively	Homo sapiens

Crystallization (Comment)

Organism

wild-type and selenomethionine enzyme, 0.001 ml of protein and of reservoir solution, the latter containing 16% PEG 4000 w/v, 0.18 M ammonium sulfate, 80 mM sodium acetate, pH 4.6, 20% glycerol, addition of 30% ethylene glycol and 4 mM NADP+ for the binary complex of enzyme with NADP+, and 120 mM substrate for the ternary complex of enzyme with NADP+ and substrate trans-trans-2,4-dienoyl-CoA, X-ray diffraction structure determination and analysis at 2.1 A and 1.75 A resolution, respectively

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
E310A	site-directed mutagenesis, effects on reaction mechanism	Homo sapiens
E310Q	site-directed mutagenesis, effects on reaction mechanism	Homo sapiens
K214A	site-directed mutagenesis, effects on reaction mechanism	Homo sapiens
N148A	site-directed mutagenesis, effects on reaction mechanism	Homo sapiens
S210A	site-directed mutagenesis, effects on reaction mechanism	Homo sapiens
T197A	site-directed mutagenesis, effects on reaction mechanism	Homo sapiens
Y199A	site-directed mutagenesis, effects on reaction mechanism	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Homo sapiens	5739	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	enzyme is the key enzyme of beta-oxidation of unsaturated fatty acid in mitochondria and to a lesser extent in peroxisomes, three accessory proteins are involved in the pathway	?	-	?
trans-trans-2,4-hexadienoyl-CoA + NADPH	Homo sapiens	-	trans-3-hexenoyl-CoA + NADP+	-	r

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, to homogeneity	Homo sapiens

Reaction

Reaction	Comment	Organism	Reaction ID
a (2E)-2-enoyl-CoA + NADP+ = a (2E,4E)-2,4-dienoyl-CoA + NADPH + H+	reaction mechanism, enzyme-ligand interactions in a distinctive short-chain reductase active site, catalytic site structure, stabilization of the dienolate reaction intermediate by Tyr199 and Asn148, and NADP+, overview	Homo sapiens	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
additional information	enzyme is the key enzyme of beta-oxidation of unsaturated fatty acid in mitochondria and to a lesser extent in peroxisomes, three accessory proteins are involved in the pathway	Homo sapiens	?	-	?
trans-trans-2,4-hexadienoyl-CoA + NADPH	-	Homo sapiens	trans-3-hexenoyl-CoA + NADP+	-	r
trans-trans-2,4-hexadienoyl-CoA + NADPH	substrate binding mechanism and structure	Homo sapiens	trans-3-hexenoyl-CoA + NADP+	-	r

Subunits

Subunits	Comment	Organism
tetramer	crystal structure	Homo sapiens

Synonyms

Synonyms	Comment	Organism
2,4-dienoyl-CoA reductase	-	Homo sapiens
DECR	-	Homo sapiens
More	enzyme belongs to the short-chain dehydrogenase/reductase family with a distinctive catalytic center	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at room temperature	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	binding mechanism and structure	Homo sapiens
NADPH	binding mechanism and structure	Homo sapiens