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Literature summary for 1.3.1.123 extracted from

  • Fellows, R.; Russo, C.M.; Silva, C.S.; Lee, S.G.; Jez, J.M.; Chisholm, J.D.; Zubieta, C.; Nanao, M.H.
    A multisubstrate reductase from Plantago major structure-function in the short chain reductase superfamily (2018), Sci. Rep., 8, 14796 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.123

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Plantago major

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with NADP+ and 8-oxogeranial, hanging drop vapor diffusion method, using 0.1 M Bis-Tris Propane, 5% (v/v) glycerol, and 16.5-19% (w/v) PEG 5000 MME Plantago major

Protein Variants

Protein Variants Comment Organism
V150M the mutant shows increased specific activity with respect to progesterone, accepting both citral and progesterone as substrate, with a clear preference for citral Plantago major
V150M/I156Y the double mutant shows increased specific activity with respect to progesterone, accepting both citral and progesterone as substrate, with a clear preference for citral Plantago major

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.368
-
 (6E)-8-oxogeranial at pH 7.0, temperature not specified in the publication Plantago major

Organism

Organism UniProt Comment Textmining
Plantago major
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex S200 gel filtration Plantago major

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(6E)-8-oxogeranial + NADPH + H+
-
 Plantago major (R)-8-oxocitronellyl enol + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
MOR
-
 Plantago major
multisubstrate oxido-reductase
-
 Plantago major

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6
-
 (6E)-8-oxogeranial at pH 7.0, temperature not specified in the publication Plantago major

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Plantago major