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Literature summary for 1.3.1.123 extracted from

  • Xiang, B.; Li, X.; Wang, Y.; Tian, X.; Yang, Z.; Ma, L.; Liu, X.; Wang, Y.
    Cloning and characterization of two iridoid synthase homologs from Swertia mussotii (2017), Molecules, 22, E1387 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.123

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Swertia mussotii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00729
-
 (6E)-8-oxogeranial isoform IS1, at pH 7.0 and 25°C Swertia mussotii
0.04183
-
 NADPH isoform IS2, at pH 7.0 and 25°C Swertia mussotii
0.05437
-
 (6E)-8-oxogeranial isoform IS2, at pH 7.0 and 25°C Swertia mussotii
0.07794
-
 NADPH isoform IS1, at pH 7.0 and 25°C Swertia mussotii

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Swertia mussotii 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(6E)-8-oxogeranial + NADPH + H+ Swertia mussotii isoform IS2 has a lower affinity than IS1 for (6E)-8-oxogeranial nepetalactol + iridodial + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Swertia mussotii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His Trap column chromatography Swertia mussotii

Source Tissue

Source Tissue Comment Organism Textmining
flower lowest expression Swertia mussotii
-
leaf highest expression Swertia mussotii
-
stem
-
 Swertia mussotii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(6E)-8-oxogeranial + NADPH + H+ isoform IS2 has a lower affinity than IS1 for (6E)-8-oxogeranial Swertia mussotii nepetalactol + iridodial + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 46000, isoform IS2, SDS-PAGE Swertia mussotii
? x * 88000, isoform IS1, SDS-PAGE Swertia mussotii

Synonyms

Synonyms Comment Organism
iridoid synthase
-
 Swertia mussotii
IS1 isoform Swertia mussotii
IS2 isoform Swertia mussotii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.026
-
 NADPH isoform IS2, at pH 7.0 and 25°C Swertia mussotii
0.034
-
 (6E)-8-oxogeranial isoform IS2, at pH 7.0 and 25°C Swertia mussotii
0.11
-
 (6E)-8-oxogeranial isoform IS1, at pH 7.0 and 25°C Swertia mussotii
0.135
-
 NADPH isoform IS1, at pH 7.0 and 25°C Swertia mussotii

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Swertia mussotii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.628
-
 NADPH isoform IS2, at pH 7.0 and 25°C Swertia mussotii
0.628
-
 (6E)-8-oxogeranial isoform IS2, at pH 7.0 and 25°C Swertia mussotii
1.73
-
 NADPH isoform IS1, at pH 7.0 and 25°C Swertia mussotii
15.56
-
 (6E)-8-oxogeranial isoform IS1, at pH 7.0 and 25°C Swertia mussotii