Literature summary for 1.3.1.122 extracted from

Sherden, N.; Lichman, B.; Caputi, L.; Zhao, D.; Kamileen, M.; Buell, C.; O'Connor, S.
Identification of iridoid synthases from Nepeta species Iridoid cyclization does not determine nepetalactone stereochemistry (2018), Phytochemistry, 145, 48-56 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli soluBL21 (DE3) cells	Nepeta cataria
expressed in Escherichia coli soluBL21 (DE3) cells	Nepeta racemosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0073	-	(6E)-8-oxogeranial	at pH 7.0 and 30°C	Nepeta racemosa
0.0262	-	NADPH	at pH 7.0 and 30°C	Nepeta racemosa

Organism

Organism	UniProt	Comment	Textmining
Nepeta cataria	-	-	-
Nepeta racemosa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap column chromatography and Superdex S200 gel filtration	Nepeta cataria
HisTrap column chromatography and Superdex S200 gel filtration	Nepeta racemosa

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Nepeta cataria	-
leaf	-	Nepeta racemosa	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(6E)-8-oxogeranial + NADPH + H+	-	Nepeta cataria	cis-trans-nepetalactone + NADP+	-	?
(6E)-8-oxogeranial + NADPH + H+	-	Nepeta racemosa	cis-trans-nepetalactone + NADP+	-	?
(6E)-8-oxogeranial + NADPH + H+	-	Nepeta racemosa	trans-cis-nepetalactone + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
iridoid synthase	-	Nepeta cataria
iridoid synthase	-	Nepeta racemosa
ISY	-	Nepeta cataria
ISY	-	Nepeta racemosa

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.6	-	(6E)-8-oxogeranial	at pH 7.0 and 30°C	Nepeta racemosa
0.84	-	NADPH	at pH 7.0 and 30°C	Nepeta racemosa

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Nepeta cataria
NADPH	-	Nepeta racemosa

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Release 2023.1 (January 2023)