Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | 1 mM, 52% inhibition | Streptomyces collinus | |
N-ethylmaleimide | 1 mM, 100% inhibition | Streptomyces collinus | |
NADP+ | cofactor inhibition | Streptomyces collinus | |
p-chloromercuribenzoate | 0.002 mM, 65% inhibition | Streptomyces collinus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
NADPH | pH 7.4, 30°C | Streptomyces collinus | |
0.025 | - |
cyclohexane-1-carbonyl-CoA | pH 7.4, 30°C | Streptomyces collinus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, 88% inhibition | Streptomyces collinus | |
Co2+ | 1 mM, 80% inhibition | Streptomyces collinus | |
Mg2+ | 1 mM, 58% inhibition | Streptomyces collinus | |
Mn2+ | 1 mM, 85% inhibition | Streptomyces collinus | |
Zn2+ | 1 mM, 100% inhibition | Streptomyces collinus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
gel filtration | Streptomyces collinus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces collinus | P95727 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces collinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclohex-1-ene-1-carbonyl-CoA + NADPH + H+ | - |
Streptomyces collinus | cyclohexane-1-carbonyl-CoA + NADP+ | - |
r | |
cyclohexa-1,3-diene-1-carbonyl-CoA + NADP+ | 13% of activity | Streptomyces collinus | cyclohex-3-ene-1-carbonyl-CoA + NADPH + H+ | - |
? | |
cyclohexane-1-carbonyl-CoA + NADP+ | - |
Streptomyces collinus | cyclohex-1-ene-1-carbonyl-CoA + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 36000, SDS-PAGE | Streptomyces collinus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Streptomyces collinus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Streptomyces collinus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity found with NADH | Streptomyces collinus | |
NADPH | - |
Streptomyces collinus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
NADP+ | pH 7.4, 30°C | Streptomyces collinus |
General Information | Comment | Organism |
---|---|---|
physiological function | the 1-cyclohexenylcarbonyl-CoA reductase catalyzes the final reductive step in the conversion of shikimic acid into asatrienin A | Streptomyces collinus |