Literature summary for 1.3.1.120 extracted from

Reynolds, K.A.; Wang, P.; Fox, K.M.; Speedie, M.K.; Lam, Y.; Floss, H.G.
Purification and characterization of a novel enoyl coenzyme A reductase from Streptomyces collinus (1992), J. Bacteriol., 174, 3850-3854 .

Search for more literature for 1.3.1.120

Inhibitors

Inhibitors	Comment	Organism	Structure
iodoacetamide	1 mM, 52% inhibition	Streptomyces collinus
N-ethylmaleimide	1 mM, 100% inhibition	Streptomyces collinus
NADP+	cofactor inhibition	Streptomyces collinus
p-chloromercuribenzoate	0.002 mM, 65% inhibition	Streptomyces collinus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0015	-	NADPH	pH 7.4, 30°C	Streptomyces collinus
0.025	-	cyclohexane-1-carbonyl-CoA	pH 7.4, 30°C	Streptomyces collinus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	1 mM, 88% inhibition	Streptomyces collinus
Co2+	1 mM, 80% inhibition	Streptomyces collinus
Mg2+	1 mM, 58% inhibition	Streptomyces collinus
Mn2+	1 mM, 85% inhibition	Streptomyces collinus
Zn2+	1 mM, 100% inhibition	Streptomyces collinus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
75000	-	gel filtration	Streptomyces collinus

Organism

Organism	UniProt	Comment	Textmining
Streptomyces collinus	P95727	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Streptomyces collinus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cyclohex-1-ene-1-carbonyl-CoA + NADPH + H+	-	Streptomyces collinus	cyclohexane-1-carbonyl-CoA + NADP+	-	r
cyclohexa-1,3-diene-1-carbonyl-CoA + NADP+	13% of activity	Streptomyces collinus	cyclohex-3-ene-1-carbonyl-CoA + NADPH + H+	-	?
cyclohexane-1-carbonyl-CoA + NADP+	-	Streptomyces collinus	cyclohex-1-ene-1-carbonyl-CoA + NADPH + H+	-	r

Subunits

Subunits	Comment	Organism
dimer	2 * 36000, SDS-PAGE	Streptomyces collinus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Streptomyces collinus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Streptomyces collinus

Cofactor

Cofactor	Comment	Organism	Structure
additional information	no activity found with NADH	Streptomyces collinus
NADPH	-	Streptomyces collinus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.004	-	NADP+	pH 7.4, 30°C	Streptomyces collinus

General Information

General Information	Comment	Organism
physiological function	the 1-cyclohexenylcarbonyl-CoA reductase catalyzes the final reductive step in the conversion of shikimic acid into asatrienin A	Streptomyces collinus

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Release 2023.1 (January 2023)