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Literature summary for 1.3.1.12 extracted from

  • Sun, W.; Shahinas, D.; Bonvin, J.; Hou, W.; Kimber, M.S.; Turnbull, J.; Christendat, D.
    The crystal structure of Aquifex aeolicus prephenate dehydrogenase reveals the mode of tyrosine inhibition (2009), J. Biol. Chem., 284, 13223-13232.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.12

Crystallization (Commentary)

Crystallization (Comment) Organism
prephenate dehydrogenase bound with NAD+ plus either 4-hydroxyphenylpyuvate, 4-hydroxyphenylpropionate, or L-tyrosine. Resiudes His147 and Arg250 are key catalytic and binding groups, respectively, and Ser126 participates in both catalysis and substrate binding through the ligand 4-hydroxyl group. Inhibitor tyrosine binds directly to the active site of the enzyme and not to an allosteric site Aquifex aeolicus

Protein Variants

Protein Variants Comment Organism
H147N inactive, binds prephenate with apparent affinity similar to wild-type Aquifex aeolicus
H217A 40fold increase in Km for prephenate, no inhibition by tyrosine Aquifex aeolicus
H217N 30fold increase in Km for prephenate, no inhibition by tyrosine Aquifex aeolicus
R250Q 10fold increase in the Km for prephenate and a 20fold increase in Ki for tyrosine Aquifex aeolicus
S126A 15fold reduction in kcat, a 10fold increase in the Km value for prephenate, and a 2-fold increase in Ki for tyrosine Aquifex aeolicus

Inhibitors

Inhibitors Comment Organism Structure
tyrosine binds directly to the active site of the enzyme and not to an allosteric site. Linear competitive inhibition Aquifex aeolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 NAD+ mutant H217A, pH 7.5, 55°C Aquifex aeolicus
0.012
-
 NAD+ mutant H217N, pH 7.5, 55°C Aquifex aeolicus
0.071
-
 NAD+ wild-type, pH 7.5, 55°C Aquifex aeolicus
0.089
-
 NAD+ mutant R250Q, pH 7.5, 55°C Aquifex aeolicus
0.099
-
 NAD+ mutant S126A, pH 7.5, 55°C Aquifex aeolicus
0.104
-
 prephenate mutant H147N, pH 7.5, 55°C Aquifex aeolicus
0.135
-
 prephenate wild-type, pH 7.5, 55°C Aquifex aeolicus
1.185
-
 prephenate mutant R250Q, pH 7.5, 55°C Aquifex aeolicus
1.335
-
 prephenate mutant S126A, pH 7.5, 55°C Aquifex aeolicus
3.213
-
 prephenate mutant H217N, pH 7.5, 55°C Aquifex aeolicus
4.132
-
 prephenate mutant H217A, pH 7.5, 55°C Aquifex aeolicus

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus O67636
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
prephenate + NAD+
-
 Aquifex aeolicus 4-hydroxyphenylpyruvate + CO2 + NADH
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0034
-
 prephenate mutant H147N, pH 7.5, 55°C Aquifex aeolicus
0.3
-
 NAD+ mutant H217N, pH 7.5, 55°C Aquifex aeolicus
0.5
-
 prephenate mutant H217N, pH 7.5, 55°C Aquifex aeolicus
0.6
-
 NAD+ mutant H217A, pH 7.5, 55°C Aquifex aeolicus
0.8
-
 prephenate mutant H217A, pH 7.5, 55°C Aquifex aeolicus
0.8
-
 NAD+ mutant S126A, pH 7.5, 55°C Aquifex aeolicus
0.8
-
 prephenate mutant S126A, pH 7.5, 55°C Aquifex aeolicus
9.9
-
 prephenate mutant R250Q, pH 7.5, 55°C Aquifex aeolicus
11.5
-
 NAD+ wild-type, pH 7.5, 55°C Aquifex aeolicus
11.6
-
 NAD+ mutant R250Q, pH 7.5, 55°C Aquifex aeolicus
13
-
 prephenate wild-type, pH 7.5, 55°C Aquifex aeolicus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Aquifex aeolicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0159
-
 tyrosine wild-type, pH 7.5, 55°C Aquifex aeolicus
0.037
-
 tyrosine mutant S126A, pH 7.5, 55°C Aquifex aeolicus
0.37
-
 tyrosine mutant R250Q, pH 7.5, 55°C Aquifex aeolicus