Cloned (Comment) | Organism |
---|---|
into vector pET15b and expressed in Escherichia coli BL21-Gold (DE3) | Aquifex aeolicus |
Crystallization (Comment) | Organism |
---|---|
in complex with NAD+, by hanging drop vapor diffusion technique at room temperature, one modified nucleotide-binding domain and a novel helical prephenate binding domain, active site formed at the domain interface and shared between the subunits of the dimer, access to active site may be regulated via a gated mechanism, modulated by an ionic network involving a conserved arginine, active site residues include Ser126 and Lys246 and the catalytic His147, two functional domains of chorismate mutase-prephenate dehydrogenase are interdependent | Aquifex aeolicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | delta19-PDH, delta25-PDH, and delta28-PDH, which produce stable and soluble proteins, delta36-PDH, delta52-PDH, and delta55-PDH are unstable | Aquifex aeolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by Ni-NTA column | Aquifex aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
prephenate + NAD+ | - |
Aquifex aeolicus | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | gel filtration | Aquifex aeolicus |
Synonyms | Comment | Organism |
---|---|---|
PDH | - |
Aquifex aeolicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Aquifex aeolicus |