General Stability | Organism |
---|---|
stability in Tris-HCl buffer higher than in phosphate buffer | Klebsiella aerogenes |
tyrosine stabilizes | Klebsiella aerogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | - |
Klebsiella aerogenes | |
iodoacetate | - |
Klebsiella aerogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.35 | 0.37 | prephenate | - |
Klebsiella aerogenes | |
0.6 | - |
NAD+ | - |
Klebsiella aerogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76000 | 81000 | chorismate mutase-prephenate dehydrogenase bifunctional enzyme, gel filtration, gel electrophoresis | Klebsiella aerogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
prephenate + NAD+ | Klebsiella aerogenes | biosynthesis of L-tyrosine | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
chorismate mutase-prephenate dehydrogenase bifunctional enzyme | Klebsiella aerogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Klebsiella aerogenes |
Storage Stability | Organism |
---|---|
0-4°C, 0.1 M Tris-HCl buffer, pH 7.5, 1 mM EDTA, 1 mM DTT, 1 mM tyrosine, 70% w/v ammonium sulfate, for at least 10 days | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
prephenate + NAD+ | - |
Klebsiella aerogenes | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? | |
prephenate + NAD+ | biosynthesis of L-tyrosine | Klebsiella aerogenes | 4-hydroxyphenylpyruvate + NADH + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
chorismate mutase-prephenate dehydrogenase bifunctional enzyme | complex with EC 5.4.99.5 | Klebsiella aerogenes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
dithiothreitol, protection against thermal inactivation | Klebsiella aerogenes |