Characterization of the baiH gene encoding a bile acid-inducible NADHflavin oxidoreductase from Eubacterium sp. strain VPI 12708

Franklund, C.; Baron, S.; Hylemon, P.; J. Bacteriol. 175, 3002-3012 (1993)

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

1,10-phenanthroline

2.0 mM, 42% inhibition

Clostridium scindens

Acriflavine

0.2 mg/ml, 87% inhibition, acriflavine is a mixture of 3,6-diamino-10-methylacridinium chloride and 3,6-diaminoacridine; 0.2 mM, 13% residual activity

Clostridium scindens

Cu2+

1 mM, 1% residual activity

Clostridium scindens

CuCl2

1.0 mM, 99% inhibition

Clostridium scindens

EDTA

5.0 mM, 48% inhibition; 5 mM, 52% residual activity

Clostridium scindens

HgCl2

1 mM, 97% inhibition

Clostridium scindens

iodoacetate

1.0 mM, 9% inhibition

Clostridium scindens

N-bromosuccinimide

0.2 mM, 8% residual activity; 0.2 mM, 92% inhibition

Clostridium scindens

N-ethylmaleimide

1.0 mM, 96% inhibition; 1 mM, 4% residual activity

Clostridium scindens

o-phenanthroline

5 mM, 58% residual activity

Clostridium scindens

p-chloromercuriphenyl sulfonate

0.2 mM, 5% residual activity

Clostridium scindens

p-chloromercuriphenyl sulfonic acid

0.2 mM, 95% inhibition

Clostridium scindens

Zn2+

1 mM, 27% residual activity

Clostridium scindens

ZnCl2

1.0 mM, 73% inhibition

Clostridium scindens

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

FAD + NADH + H+

-

745196

Clostridium scindens

FADH2 + NAD+

-

-

-

?

FAD + NADH + H+

-

745196

Clostridium scindens VPI 12708

FADH2 + NAD+

-

-

-

?

FAD + NADH + H+

-

745196

Clostridium scindens JCM 10418

FADH2 + NAD+

-

-

-

?

Cofactor

Commentary

Organism

Structure

FAD

-

Clostridium scindens

NADH

-

Clostridium scindens

Cofactor

Commentary

Organism

Structure

FAD

-

Clostridium scindens

NADH

-

Clostridium scindens

Inhibitors

Commentary

Organism

Structure

1,10-phenanthroline

2.0 mM, 42% inhibition

Clostridium scindens

Acriflavine

0.2 mg/ml, 87% inhibition, acriflavine is a mixture of 3,6-diamino-10-methylacridinium chloride and 3,6-diaminoacridine; 0.2 mM, 13% residual activity

Clostridium scindens

Cu2+

1 mM, 1% residual activity

Clostridium scindens

CuCl2

1.0 mM, 99% inhibition

Clostridium scindens

EDTA

5.0 mM, 48% inhibition; 5 mM, 52% residual activity

Clostridium scindens

HgCl2

1 mM, 97% inhibition

Clostridium scindens

iodoacetate

1.0 mM, 9% inhibition

Clostridium scindens

N-bromosuccinimide

0.2 mM, 8% residual activity; 0.2 mM, 92% inhibition

Clostridium scindens

N-ethylmaleimide

1.0 mM, 96% inhibition; 1 mM, 4% residual activity

Clostridium scindens

o-phenanthroline

5 mM, 58% residual activity

Clostridium scindens

p-chloromercuriphenyl sulfonate

0.2 mM, 5% residual activity

Clostridium scindens

p-chloromercuriphenyl sulfonic acid

0.2 mM, 95% inhibition

Clostridium scindens

Zn2+

1 mM, 27% residual activity

Clostridium scindens

ZnCl2

1.0 mM, 73% inhibition

Clostridium scindens

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

FAD + NADH + H+

-

745196

Clostridium scindens

FADH2 + NAD+

-

-

-

?

FAD + NADH + H+

-

745196

Clostridium scindens VPI 12708

FADH2 + NAD+

-

-

-

?

FAD + NADH + H+

-

745196

Clostridium scindens JCM 10418

FADH2 + NAD+

-

-

-

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

744383

Kang

Clostridium scindens baiCD an ...

Clostridium scindens, Clostridium scindens JCM 10418, Clostridium scindens VPI 12708

Biochim. Biophys. Acta

1781

16-25

2008

-

-

1

-

-

-

-

-

-

-

1

6

-

12

-

-

1

-

-

-

1

-

9

2

3

1

-

-

-

1

-

-

5

-

-

-

-

-

1

5

-

-

-

-

-

-

-

-

-

1

6

-

-

-

1

-

-

1

-

9

2

1

-

-

-

1

-

-

-

-

1

1

-

-

-

744377

Baron

Expression of the bile acid-i ...

Clostridium scindens, Clostridium scindens JCM 10418, Clostridium scindens VPI 12708

Biochim. Biophys. Acta

1249

145-154

1995

-

-

1

-

-

-

12

-

-

2

2

-

-

7

-

-

1

-

-

-

-

-

3

2

2

1

-

-

-

1

-

-

2

-

1

-

-

-

1

2

-

-

-

-

12

-

-

-

2

2

-

-

-

-

1

-

-

-

-

3

2

1

-

-

-

1

-

-

1

-

1

1

-

-

-

745196

Franklund

Characterization of the baiH ...

Clostridium scindens, Clostridium scindens JCM 10418, Clostridium scindens VPI 12708

J. Bacteriol.

175

3002-3012

1993

-

-

1

-

-

-

14

-

-

-

2

-

-

7

-

-

1

-

-

-

1

-

3

2

1

1

-

-

-

1

-

-

2

-

-

-

-

-

1

2

-

-

-

-

14

-

-

-

-

2

-

-

-

-

1

-

-

1

-

3

2

1

-

-

-

1

-

-

-

1

-

-

1

-

-