BRENDA - Reference to 1.3.1.116; Id = 744377

expression in Escherichia coli; subcloned into an IPTG-inducible expression vector, pBaiH2.2. Escherichia coli DH5a cells transformed with pBaiH2.2 express 10fold higher levels of NADH:FOR upon induction with IPTG than does Eubacterium sp. VPI 12708 cells induced with cholic acid

Clostridium scindens

1,10-phenanthroline

-

Clostridium scindens

Acriflavine

-

Clostridium scindens

CuCl2

-

Clostridium scindens

EDTA

-

Clostridium scindens

EGTA

-

Clostridium scindens

HgCl2

-

Clostridium scindens

additional information

iodoacetate and iodoacetamide show no inhibition

Clostridium scindens

N-bromosuccinimide

-

Clostridium scindens

N-ethylmaleimide

-

Clostridium scindens

p-chloromercuriphenylsulfonic acid

-

Clostridium scindens

Sodium azide

-

Clostridium scindens

ZnCl2

-

Clostridium scindens

copper

1 mol of copper per mol of subunit; contains 1 mol of copper per subunit

Clostridium scindens

Iron

2 mol of iron per mol of subunit; contains up to 2 mol of iron per subunit

Clostridium scindens

71400

-

SDS-PAGE

Clostridium scindens

210000

-

gel filtration; non-denaturing PAGE

Clostridium scindens

P32370

-

Clostridium scindens JCM 10418

P32370

-

Clostridium scindens VPI 12708

P32370

-

; recombinant protein

Clostridium scindens

additional information

the enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor, but does not reduce disulfide compounds, various unsaturated bile acids, cytochrome c and physiological quinones

744377

Clostridium scindens

?

-

additional information

the enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor, but does not reduce disulfide compounds, various unsaturated bile acids, cytochrome c and physiological quinones

744377

Clostridium scindens VPI 12708

?

-

additional information

the enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor, but does not reduce disulfide compounds, various unsaturated bile acids, cytochrome c and physiological quinones

744377

Clostridium scindens JCM 10418

?

-

homotrimer

3 * 71400, SDS-PAGE

Clostridium scindens

trimer

3 * 72000, SDS-PAGE

Clostridium scindens

40

-

Clostridium scindens

6.8

-

Clostridium scindens

FAD

1 mol of FAD per mol of subunit; contains 1 mol of FAD

Clostridium scindens

NADH

-

Clostridium scindens

isoelectric focusing

-

6

expression in Escherichia coli; subcloned into an IPTG-inducible expression vector, pBaiH2.2. Escherichia coli DH5a cells transformed with pBaiH2.2 express 10fold higher levels of NADH:FOR upon induction with IPTG than does Eubacterium sp. VPI 12708 cells induced with cholic acid

Clostridium scindens

FAD

1 mol of FAD per mol of subunit; contains 1 mol of FAD

Clostridium scindens

NADH

-

Clostridium scindens

1,10-phenanthroline

-

Clostridium scindens

Acriflavine

-

Clostridium scindens

CuCl2

-

Clostridium scindens

EDTA

-

Clostridium scindens

EGTA

-

Clostridium scindens

HgCl2

-

Clostridium scindens

additional information

iodoacetate and iodoacetamide show no inhibition

Clostridium scindens

N-bromosuccinimide

-

Clostridium scindens

N-ethylmaleimide

-

Clostridium scindens

p-chloromercuriphenylsulfonic acid

-

Clostridium scindens

Sodium azide

-

Clostridium scindens

ZnCl2

-

Clostridium scindens

copper

1 mol of copper per mol of subunit; contains 1 mol of copper per subunit

Clostridium scindens

Iron

2 mol of iron per mol of subunit; contains up to 2 mol of iron per subunit

Clostridium scindens

71400

-

SDS-PAGE

Clostridium scindens

210000

-

gel filtration; non-denaturing PAGE

Clostridium scindens

; recombinant protein

Clostridium scindens

additional information

the enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor, but does not reduce disulfide compounds, various unsaturated bile acids, cytochrome c and physiological quinones

744377

Clostridium scindens

?

-

additional information

the enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor, but does not reduce disulfide compounds, various unsaturated bile acids, cytochrome c and physiological quinones

744377

Clostridium scindens VPI 12708

?

-

additional information

the enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor, but does not reduce disulfide compounds, various unsaturated bile acids, cytochrome c and physiological quinones

744377

Clostridium scindens JCM 10418

?

-

homotrimer

3 * 71400, SDS-PAGE

Clostridium scindens

trimer

3 * 72000, SDS-PAGE

Clostridium scindens

40

-

Clostridium scindens

6.8

-

Clostridium scindens

isoelectric focusing

-

6

physiological function

enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor. Addition of purified enzyme to Clostridium scindens cell extracts alters the balance of oxidized and reduced bile acid intermediates produced during cholic acid 7alpha-dehydroxylation

Clostridium scindens

physiological function

enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor. Addition of purified enzyme to Clostridium scindens cell extracts alters the balance of oxidized and reduced bile acid intermediates produced during cholic acid 7alpha-dehydroxylation

Clostridium scindens

744383

Kang

Clostridium scindens baiCD an ...

Clostridium scindens, Clostridium scindens JCM 10418, Clostridium scindens VPI 12708

Biochim. Biophys. Acta

1781

16-25

2008

-

1

-

1

6

-

12

-

1

-

1

-

9

2

1

-

1

-

5

-

1

5

-

1

6

-

1

-

1

-

9

2

1

-

1

-

1

-

744377

Baron

Expression of the bile acid-i ...

Clostridium scindens, Clostridium scindens JCM 10418, Clostridium scindens VPI 12708

Biochim. Biophys. Acta

1249

145-154

1995

-

1

-

12

-

2

-

7

-

1

-

3

2

1

-

1

-

2

-

1

-

1

2

-

12

-

2

-

1

-

3

2

1

-

1

-

1

-

1

-

745196

Franklund

Characterization of the baiH ...

Clostridium scindens, Clostridium scindens JCM 10418, Clostridium scindens VPI 12708

J. Bacteriol.

175

3002-3012

1993

-

1

-

14

-

2

-

7

-

1

-

1

-

3

2

1

-

1

-

2

-

1

2

-

14

-

2

-

1

-

1

-

3

2

1

-

1

-

1

-

1

-

Expression of the bile acid-inducible NADHflavin oxidoreductase gene of Eubacterium sp. VPI 12708 in Escherichia coli

Data extracted from this reference: