Data extracted from this reference:
Cloned(Commentary)
expression in Escherichia coli; subcloned into an IPTG-inducible expression vector, pBaiH2.2. Escherichia coli DH5a cells transformed with pBaiH2.2 express 10fold higher levels of NADH:FOR upon induction with IPTG than does Eubacterium sp. VPI 12708 cells induced with cholic acid
Clostridium scindens
Inhibitors
1,10-phenanthroline
Clostridium scindens
Acriflavine
Clostridium scindens
CuCl2
Clostridium scindens
EDTA
Clostridium scindens
EGTA
Clostridium scindens
HgCl2
Clostridium scindens
additional information
iodoacetate and iodoacetamide show no inhibition
Clostridium scindens
N-bromosuccinimide
Clostridium scindens
N-ethylmaleimide
Clostridium scindens
p-chloromercuriphenylsulfonic acid
Clostridium scindens
Sodium azide
Clostridium scindens
ZnCl2
Clostridium scindens
Metals/Ions
copper
1 mol of copper per mol of subunit; contains 1 mol of copper per subunit
Clostridium scindens
Iron
2 mol of iron per mol of subunit; contains up to 2 mol of iron per subunit
Clostridium scindens
Molecular Weight [Da]
71400
SDS-PAGE
Clostridium scindens
210000
gel filtration; non-denaturing PAGE
Clostridium scindens
Organism
Clostridium scindens
P32370
Clostridium scindens JCM 10418
P32370
Clostridium scindens VPI 12708
P32370
Purification (Commentary)
; recombinant protein
Clostridium scindens
Substrates and Products (Substrate)
additional information
the enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor, but does not reduce disulfide compounds, various unsaturated bile acids, cytochrome c and physiological quinones
744377
Clostridium scindens
?
additional information
the enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor, but does not reduce disulfide compounds, various unsaturated bile acids, cytochrome c and physiological quinones
744377
Clostridium scindens VPI 12708
?
additional information
the enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor, but does not reduce disulfide compounds, various unsaturated bile acids, cytochrome c and physiological quinones
744377
Clostridium scindens JCM 10418
?
Subunits
homotrimer
3 * 71400, SDS-PAGE
Clostridium scindens
trimer
3 * 72000, SDS-PAGE
Clostridium scindens
Synonyms
BaiH
Clostridium scindens
Temperature Optimum [°C]
pH Optimum
Cofactor
FAD
1 mol of FAD per mol of subunit; contains 1 mol of FAD
Clostridium scindens
NADH
Clostridium scindens
pI Value
Clostridium scindens
isoelectric focusing
6
Cloned(Commentary) (protein specific)
expression in Escherichia coli; subcloned into an IPTG-inducible expression vector, pBaiH2.2. Escherichia coli DH5a cells transformed with pBaiH2.2 express 10fold higher levels of NADH:FOR upon induction with IPTG than does Eubacterium sp. VPI 12708 cells induced with cholic acid
Clostridium scindens
Cofactor (protein specific)
FAD
1 mol of FAD per mol of subunit; contains 1 mol of FAD
Clostridium scindens
NADH
Clostridium scindens
Inhibitors (protein specific)
1,10-phenanthroline
Clostridium scindens
Acriflavine
Clostridium scindens
CuCl2
Clostridium scindens
EDTA
Clostridium scindens
EGTA
Clostridium scindens
HgCl2
Clostridium scindens
additional information
iodoacetate and iodoacetamide show no inhibition
Clostridium scindens
N-bromosuccinimide
Clostridium scindens
N-ethylmaleimide
Clostridium scindens
p-chloromercuriphenylsulfonic acid
Clostridium scindens
Sodium azide
Clostridium scindens
ZnCl2
Clostridium scindens
Metals/Ions (protein specific)
copper
1 mol of copper per mol of subunit; contains 1 mol of copper per subunit
Clostridium scindens
Iron
2 mol of iron per mol of subunit; contains up to 2 mol of iron per subunit
Clostridium scindens
Molecular Weight [Da] (protein specific)
71400
SDS-PAGE
Clostridium scindens
210000
gel filtration; non-denaturing PAGE
Clostridium scindens
Purification (Commentary) (protein specific)
; recombinant protein
Clostridium scindens
Substrates and Products (Substrate) (protein specific)
additional information
the enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor, but does not reduce disulfide compounds, various unsaturated bile acids, cytochrome c and physiological quinones
744377
Clostridium scindens
?
additional information
the enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor, but does not reduce disulfide compounds, various unsaturated bile acids, cytochrome c and physiological quinones
744377
Clostridium scindens VPI 12708
?
additional information
the enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor, but does not reduce disulfide compounds, various unsaturated bile acids, cytochrome c and physiological quinones
744377
Clostridium scindens JCM 10418
?
Subunits (protein specific)
homotrimer
3 * 71400, SDS-PAGE
Clostridium scindens
trimer
3 * 72000, SDS-PAGE
Clostridium scindens
Temperature Optimum [°C] (protein specific)
pH Optimum (protein specific)
pI Value (protein specific)
Clostridium scindens
isoelectric focusing
6
General Information
physiological function
enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor. Addition of purified enzyme to Clostridium scindens cell extracts alters the balance of oxidized and reduced bile acid intermediates produced during cholic acid 7alpha-dehydroxylation
Clostridium scindens
General Information (protein specific)
physiological function
enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor. Addition of purified enzyme to Clostridium scindens cell extracts alters the balance of oxidized and reduced bile acid intermediates produced during cholic acid 7alpha-dehydroxylation
Clostridium scindens
Other publictions for EC 1.3.1.116
744383
Kang
Clostridium scindens baiCD an ...
Clostridium scindens, Clostridium scindens JCM 10418, Clostridium scindens VPI 12708
Biochim. Biophys. Acta
1781
16-25
2008
-
-
1
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-
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-
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-
-
1
6
-
12
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1
-
-
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1
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9
2
3
1
-
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1
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-
5
-
-
-
-
-
1
5
-
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1
6
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1
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1
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9
2
1
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1
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1
1
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-
744377
Baron
Expression of the bile acid-i ...
Clostridium scindens, Clostridium scindens JCM 10418, Clostridium scindens VPI 12708
Biochim. Biophys. Acta
1249
145-154
1995
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-
1
-
-
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12
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2
2
-
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7
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1
-
-
-
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3
2
2
1
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-
1
-
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2
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1
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1
2
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12
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2
2
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1
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3
2
1
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1
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1
-
1
1
-
-
-
745196
Franklund
Characterization of the baiH ...
Clostridium scindens, Clostridium scindens JCM 10418, Clostridium scindens VPI 12708
J. Bacteriol.
175
3002-3012
1993
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1
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14
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2
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7
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1
-
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1
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3
2
1
1
-
-
-
1
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2
-
-
-
-
-
1
2
-
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-
-
14
-
-
-
-
2
-
-
-
-
1
-
-
1
-
3
2
1
-
-
-
1
-
-
-
1
-
-
1
-
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