BRENDA - Enzyme Database
show all sequences of 1.3.1.116

Expression of the bile acid-inducible NADHflavin oxidoreductase gene of Eubacterium sp. VPI 12708 in Escherichia coli

Baron, S.; Hylemon, P.; Biochim. Biophys. Acta 1249, 145-154 (1995)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli; subcloned into an IPTG-inducible expression vector, pBaiH2.2. Escherichia coli DH5a cells transformed with pBaiH2.2 express 10fold higher levels of NADH:FOR upon induction with IPTG than does Eubacterium sp. VPI 12708 cells induced with cholic acid
Clostridium scindens
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Clostridium scindens
Acriflavine
-
Clostridium scindens
CuCl2
-
Clostridium scindens
EDTA
-
Clostridium scindens
EGTA
-
Clostridium scindens
HgCl2
-
Clostridium scindens
additional information
iodoacetate and iodoacetamide show no inhibition
Clostridium scindens
N-bromosuccinimide
-
Clostridium scindens
N-ethylmaleimide
-
Clostridium scindens
p-chloromercuriphenylsulfonic acid
-
Clostridium scindens
Sodium azide
-
Clostridium scindens
ZnCl2
-
Clostridium scindens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
copper
1 mol of copper per mol of subunit; contains 1 mol of copper per subunit
Clostridium scindens
Iron
2 mol of iron per mol of subunit; contains up to 2 mol of iron per subunit
Clostridium scindens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
71400
-
SDS-PAGE
Clostridium scindens
210000
-
gel filtration; non-denaturing PAGE
Clostridium scindens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Clostridium scindens
P32370
-
-
Clostridium scindens JCM 10418
P32370
-
-
Clostridium scindens VPI 12708
P32370
-
-
Purification (Commentary)
Commentary
Organism
; recombinant protein
Clostridium scindens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor, but does not reduce disulfide compounds, various unsaturated bile acids, cytochrome c and physiological quinones
744377
Clostridium scindens
?
-
-
-
-
additional information
the enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor, but does not reduce disulfide compounds, various unsaturated bile acids, cytochrome c and physiological quinones
744377
Clostridium scindens VPI 12708
?
-
-
-
-
additional information
the enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor, but does not reduce disulfide compounds, various unsaturated bile acids, cytochrome c and physiological quinones
744377
Clostridium scindens JCM 10418
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
homotrimer
3 * 71400, SDS-PAGE
Clostridium scindens
trimer
3 * 72000, SDS-PAGE
Clostridium scindens
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Clostridium scindens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
-
Clostridium scindens
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
1 mol of FAD per mol of subunit; contains 1 mol of FAD
Clostridium scindens
NADH
-
Clostridium scindens
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Clostridium scindens
isoelectric focusing
-
6
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli; subcloned into an IPTG-inducible expression vector, pBaiH2.2. Escherichia coli DH5a cells transformed with pBaiH2.2 express 10fold higher levels of NADH:FOR upon induction with IPTG than does Eubacterium sp. VPI 12708 cells induced with cholic acid
Clostridium scindens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
1 mol of FAD per mol of subunit; contains 1 mol of FAD
Clostridium scindens
NADH
-
Clostridium scindens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Clostridium scindens
Acriflavine
-
Clostridium scindens
CuCl2
-
Clostridium scindens
EDTA
-
Clostridium scindens
EGTA
-
Clostridium scindens
HgCl2
-
Clostridium scindens
additional information
iodoacetate and iodoacetamide show no inhibition
Clostridium scindens
N-bromosuccinimide
-
Clostridium scindens
N-ethylmaleimide
-
Clostridium scindens
p-chloromercuriphenylsulfonic acid
-
Clostridium scindens
Sodium azide
-
Clostridium scindens
ZnCl2
-
Clostridium scindens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
copper
1 mol of copper per mol of subunit; contains 1 mol of copper per subunit
Clostridium scindens
Iron
2 mol of iron per mol of subunit; contains up to 2 mol of iron per subunit
Clostridium scindens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
71400
-
SDS-PAGE
Clostridium scindens
210000
-
gel filtration; non-denaturing PAGE
Clostridium scindens
Purification (Commentary) (protein specific)
Commentary
Organism
; recombinant protein
Clostridium scindens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor, but does not reduce disulfide compounds, various unsaturated bile acids, cytochrome c and physiological quinones
744377
Clostridium scindens
?
-
-
-
-
additional information
the enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor, but does not reduce disulfide compounds, various unsaturated bile acids, cytochrome c and physiological quinones
744377
Clostridium scindens VPI 12708
?
-
-
-
-
additional information
the enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor, but does not reduce disulfide compounds, various unsaturated bile acids, cytochrome c and physiological quinones
744377
Clostridium scindens JCM 10418
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
homotrimer
3 * 71400, SDS-PAGE
Clostridium scindens
trimer
3 * 72000, SDS-PAGE
Clostridium scindens
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Clostridium scindens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
-
Clostridium scindens
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Clostridium scindens
isoelectric focusing
-
6
General Information
General Information
Commentary
Organism
physiological function
enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor. Addition of purified enzyme to Clostridium scindens cell extracts alters the balance of oxidized and reduced bile acid intermediates produced during cholic acid 7alpha-dehydroxylation
Clostridium scindens
General Information (protein specific)
General Information
Commentary
Organism
physiological function
enzyme reduces synthetic quinones, dyes, flavins, and O2 with NADH as the electron donor. Addition of purified enzyme to Clostridium scindens cell extracts alters the balance of oxidized and reduced bile acid intermediates produced during cholic acid 7alpha-dehydroxylation
Clostridium scindens
Other publictions for EC 1.3.1.116
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744383
Kang
Clostridium scindens baiCD an ...
Clostridium scindens, Clostridium scindens JCM 10418, Clostridium scindens VPI 12708
Biochim. Biophys. Acta
1781
16-25
2008
-
-
1
-
-
-
-
-
-
-
1
6
-
12
-
-
1
-
-
-
1
-
9
2
1
-
-
-
1
-
-
5
-
-
-
-
-
1
5
-
-
-
-
-
-
-
-
-
1
6
-
-
-
1
-
-
1
-
9
2
1
-
-
-
1
-
-
-
-
1
1
-
-
-
744377
Baron
Expression of the bile acid-i ...
Clostridium scindens, Clostridium scindens JCM 10418, Clostridium scindens VPI 12708
Biochim. Biophys. Acta
1249
145-154
1995
-
-
1
-
-
-
12
-
-
2
2
-
-
7
-
-
1
-
-
-
-
-
3
2
1
-
-
-
1
-
-
2
-
1
-
-
-
1
2
-
-
-
-
12
-
-
-
2
2
-
-
-
-
1
-
-
-
-
3
2
1
-
-
-
1
-
-
1
-
1
1
-
-
-
745196
Franklund
Characterization of the baiH ...
Clostridium scindens, Clostridium scindens JCM 10418, Clostridium scindens VPI 12708
J. Bacteriol.
175
3002-3012
1993
-
-
1
-
-
-
14
-
-
-
2
-
-
7
-
-
1
-
-
-
1
-
3
2
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
14
-
-
-
-
2
-
-
-
-
1
-
-
1
-
3
2
1
-
-
-
1
-
-
-
1
-
-
1
-
-