BRENDA - Enzyme Database
show all sequences of 1.3.1.116

Characterization of the baiH gene encoding a bile acid-inducible NADHflavin oxidoreductase from Eubacterium sp. strain VPI 12708

Franklund, C.; Baron, S.; Hylemon, P.; J. Bacteriol. 175, 3002-3012 (1993)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
-
Clostridium scindens
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
2.0 mM, 42% inhibition
Clostridium scindens
Acriflavine
0.2 mg/ml, 87% inhibition, acriflavine is a mixture of 3,6-diamino-10-methylacridinium chloride and 3,6-diaminoacridine; 0.2 mM, 13% residual activity
Clostridium scindens
Cu2+
1 mM, 1% residual activity
Clostridium scindens
CuCl2
1.0 mM, 99% inhibition
Clostridium scindens
EDTA
5.0 mM, 48% inhibition; 5 mM, 52% residual activity
Clostridium scindens
HgCl2
1 mM, 97% inhibition
Clostridium scindens
iodoacetate
1.0 mM, 9% inhibition
Clostridium scindens
N-bromosuccinimide
0.2 mM, 8% residual activity; 0.2 mM, 92% inhibition
Clostridium scindens
N-ethylmaleimide
1.0 mM, 96% inhibition; 1 mM, 4% residual activity
Clostridium scindens
o-phenanthroline
5 mM, 58% residual activity
Clostridium scindens
p-chloromercuriphenyl sulfonate
0.2 mM, 5% residual activity
Clostridium scindens
p-chloromercuriphenyl sulfonic acid
0.2 mM, 95% inhibition
Clostridium scindens
Zn2+
1 mM, 27% residual activity
Clostridium scindens
ZnCl2
1.0 mM, 73% inhibition
Clostridium scindens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
72000
-
SDS-PAGE
Clostridium scindens
210000
-
gel filtration
Clostridium scindens
Organism
Organism
UniProt
Commentary
Textmining
Clostridium scindens
P32370
-
-
Clostridium scindens JCM 10418
P32370
-
-
Clostridium scindens VPI 12708
P32370
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Clostridium scindens
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
48.36
-
pH 6.8, 20C
Clostridium scindens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
FAD + NADH + H+
-
745196
Clostridium scindens
FADH2 + NAD+
-
-
-
?
FAD + NADH + H+
-
745196
Clostridium scindens VPI 12708
FADH2 + NAD+
-
-
-
?
FAD + NADH + H+
-
745196
Clostridium scindens JCM 10418
FADH2 + NAD+
-
-
-
?
Subunits
Subunits
Commentary
Organism
homotrimer
3 * 72000, SDS-PAGE
Clostridium scindens
trimer
3 * 72000, SDS-PAGE, x * 72006, calculated from sequence
Clostridium scindens
Synonyms
Synonyms
Commentary
Organism
BaiH
-
Clostridium scindens
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
20
-
assay at
Clostridium scindens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
assay at
Clostridium scindens
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Clostridium scindens
NADH
-
Clostridium scindens
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Clostridium scindens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Clostridium scindens
NADH
-
Clostridium scindens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
2.0 mM, 42% inhibition
Clostridium scindens
Acriflavine
0.2 mg/ml, 87% inhibition, acriflavine is a mixture of 3,6-diamino-10-methylacridinium chloride and 3,6-diaminoacridine; 0.2 mM, 13% residual activity
Clostridium scindens
Cu2+
1 mM, 1% residual activity
Clostridium scindens
CuCl2
1.0 mM, 99% inhibition
Clostridium scindens
EDTA
5.0 mM, 48% inhibition; 5 mM, 52% residual activity
Clostridium scindens
HgCl2
1 mM, 97% inhibition
Clostridium scindens
iodoacetate
1.0 mM, 9% inhibition
Clostridium scindens
N-bromosuccinimide
0.2 mM, 8% residual activity; 0.2 mM, 92% inhibition
Clostridium scindens
N-ethylmaleimide
1.0 mM, 96% inhibition; 1 mM, 4% residual activity
Clostridium scindens
o-phenanthroline
5 mM, 58% residual activity
Clostridium scindens
p-chloromercuriphenyl sulfonate
0.2 mM, 5% residual activity
Clostridium scindens
p-chloromercuriphenyl sulfonic acid
0.2 mM, 95% inhibition
Clostridium scindens
Zn2+
1 mM, 27% residual activity
Clostridium scindens
ZnCl2
1.0 mM, 73% inhibition
Clostridium scindens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
72000
-
SDS-PAGE
Clostridium scindens
210000
-
gel filtration
Clostridium scindens
Purification (Commentary) (protein specific)
Commentary
Organism
-
Clostridium scindens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
48.36
-
pH 6.8, 20C
Clostridium scindens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
FAD + NADH + H+
-
745196
Clostridium scindens
FADH2 + NAD+
-
-
-
?
FAD + NADH + H+
-
745196
Clostridium scindens VPI 12708
FADH2 + NAD+
-
-
-
?
FAD + NADH + H+
-
745196
Clostridium scindens JCM 10418
FADH2 + NAD+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotrimer
3 * 72000, SDS-PAGE
Clostridium scindens
trimer
3 * 72000, SDS-PAGE, x * 72006, calculated from sequence
Clostridium scindens
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
20
-
assay at
Clostridium scindens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
assay at
Clostridium scindens
Expression
Organism
Commentary
Expression
Clostridium scindens
inducible by cholate
up
Expression (protein specific)
Organism
Commentary
Expression
Clostridium scindens
inducible by cholate
up
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744383
Kang
Clostridium scindens baiCD an ...
Clostridium scindens, Clostridium scindens JCM 10418, Clostridium scindens VPI 12708
Biochim. Biophys. Acta
1781
16-25
2008
-
-
1
-
-
-
-
-
-
-
1
6
-
12
-
-
1
-
-
-
1
-
9
2
3
1
-
-
-
1
-
-
5
-
-
-
-
-
1
5
-
-
-
-
-
-
-
-
-
1
6
-
-
-
1
-
-
1
-
9
2
1
-
-
-
1
-
-
-
-
1
1
-
-
-
744377
Baron
Expression of the bile acid-i ...
Clostridium scindens, Clostridium scindens JCM 10418, Clostridium scindens VPI 12708
Biochim. Biophys. Acta
1249
145-154
1995
-
-
1
-
-
-
12
-
-
2
2
-
-
7
-
-
1
-
-
-
-
-
3
2
2
1
-
-
-
1
-
-
2
-
1
-
-
-
1
2
-
-
-
-
12
-
-
-
2
2
-
-
-
-
1
-
-
-
-
3
2
1
-
-
-
1
-
-
1
-
1
1
-
-
-
745196
Franklund
Characterization of the baiH ...
Clostridium scindens, Clostridium scindens JCM 10418, Clostridium scindens VPI 12708
J. Bacteriol.
175
3002-3012
1993
-
-
1
-
-
-
14
-
-
-
2
-
-
7
-
-
1
-
-
-
1
-
3
2
1
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
14
-
-
-
-
2
-
-
-
-
1
-
-
1
-
3
2
1
-
-
-
1
-
-
-
1
-
-
1
-
-