BRENDA - Enzyme Database
show all sequences of 1.3.1.112

Structure and epimerase activity of anthocyanidin reductase from Vitis vinifera

Gargouri, M.; Manigand, C.; Mauge, C.; Granier, T.; Langlois dEstaintot, B.; Cala, O.; Pianet, I.; Bathany, K.; Chaudiere, J.; Gallois, B.; Acta Crystallogr. Sect. D 65, 989-1000 (2009)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Vitis vinifera
Crystallization (Commentary)
Crystallization (Commentary)
Organism
to 2.2 A resolution. The active site is wide open with the side chains of the catalytic residues Tyr168 and Lys172 turned away from the nucleotide-binding site
Vitis vinifera
Inhibitors
Inhibitors
Commentary
Organism
Structure
sodium chloride
complete inhibition above 200 mM
Vitis vinifera
Organism
Organism
UniProt
Commentary
Textmining
Vitis vinifera
Q5FB34
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
cyanidin + 2 NADPH + 2 H+
-
695331
Vitis vinifera
(+)-epicatechin + (-)-catechin + 2 NADP+
the relative concentration of catechin versus epicatechin remains nearly constant and is close to 1
-
-
?
additional information
ANR is strictly pro-S stereospecific and the reaction mechanism involves two hydride transfers from two distinct NADPH molecules. ANR produces a 50:50 mixture of 2,3-cis and 2,3-trans flavan-3-ols, i.e. 2S,3S- and 2S,3R-flavan-3-ols
695331
Vitis vinifera
?
-
-
-
-
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Vitis vinifera
Crystallization (Commentary) (protein specific)
Crystallization
Organism
to 2.2 A resolution. The active site is wide open with the side chains of the catalytic residues Tyr168 and Lys172 turned away from the nucleotide-binding site
Vitis vinifera
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
sodium chloride
complete inhibition above 200 mM
Vitis vinifera
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
cyanidin + 2 NADPH + 2 H+
-
695331
Vitis vinifera
(+)-epicatechin + (-)-catechin + 2 NADP+
the relative concentration of catechin versus epicatechin remains nearly constant and is close to 1
-
-
?
additional information
ANR is strictly pro-S stereospecific and the reaction mechanism involves two hydride transfers from two distinct NADPH molecules. ANR produces a 50:50 mixture of 2,3-cis and 2,3-trans flavan-3-ols, i.e. 2S,3S- and 2S,3R-flavan-3-ols
695331
Vitis vinifera
?
-
-
-
-
Other publictions for EC 1.3.1.112
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
711347
Gargouri
The epimerase activity of anth ...
Vitis vinifera
Biol. Chem.
391
219-227
2010
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1
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4
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4
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716027
Lacampagne
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Involvement of abscisic acid i ...
Vitis vinifera
J. Plant Growth Regul.
29
81-90
2010
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1
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1
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1
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695331
Gargouri
Structure and epimerase activi ...
Vitis vinifera
Acta Crystallogr. Sect. D
65
989-1000
2009
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1
1
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695887
Gargouri
Binding-equilibrium and kineti ...
Vitis vinifera
Arch. Biochem. Biophys.
491
61-68
2009
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2
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1
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2
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1
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739752
He
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Preparation and biological app ...
Vitis vinifera
Vitis
48
69-75
2009
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1
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676650
Pfeiffer
Biosynthesis of flavan 3-ols b ...
Vitis vinifera
Plant Physiol. Biochem.
44
323-334
2006
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1
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5
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5
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1
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1
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676588
Bogs
Proanthocyanidin synthesis and ...
Vitis vinifera
Plant Physiol.
139
652-663
2005
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1
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4
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4
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737415
Fujita
-
Anthocyanidin reductase gene e ...
Vitis vinifera
Am. J. Enol. Vitic.
56
336-342
2005
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1
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