Literature summary for 1.3.1.111 extracted from

Addlesee, H.A.; Gibson, L.C.; Jensen, P.E.; Hunter, C.N.
Cloning, sequencing and functional assignment of the chlorophyll biosynthesis gene, chlP, of Synechocystis sp. PCC 6803 (1996), FEBS Lett., 389, 126-130.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene bchP, sequence comparison with gene chlP from Synechocystis sp. strain PCC 6803	Cereibacter sphaeroides
gene chfP, DNA and amino acid sequence determination and analysis, the enzyme partially complements a bchP mutant T6G5 of purple photosynthetic bacterium Rhodobacter sphaeroides, which is blocked in the terminal hydrogenation steps of bacteriochlorophyll a biosynthesis. The mutant possesses only bacteriochlorophyll esterified with geranylgeraniol and has a reduced cellular level of the light-harvesting LH2 complex. Upon heterologous expression of the Synechocystis bchP homologue, not only are hydrogenated forms of geranylgeranyl bacteriochlorophyllide a (bchlaGG) detectable, but the level of LH2 is increased, sequence comparison with gene chP from Rhodobacter sphaeroides strain NCIB 8253	Synechocystis sp.

Cloned (Comment)

Organism

gene bchP, sequence comparison with gene chlP from Synechocystis sp. strain PCC 6803

Cereibacter sphaeroides

gene chfP, DNA and amino acid sequence determination and analysis, the enzyme partially complements a bchP mutant T6G5 of purple photosynthetic bacterium Rhodobacter sphaeroides, which is blocked in the terminal hydrogenation steps of bacteriochlorophyll a biosynthesis. The mutant possesses only bacteriochlorophyll esterified with geranylgeraniol and has a reduced cellular level of the light-harvesting LH2 complex. Upon heterologous expression of the Synechocystis bchP homologue, not only are hydrogenated forms of geranylgeranyl bacteriochlorophyllide a (bchlaGG) detectable, but the level of LH2 is increased, sequence comparison with gene chP from Rhodobacter sphaeroides strain NCIB 8253

Synechocystis sp.

Protein Variants

Protein Variants	Comment	Organism
additional information	the bchP mutant T6G5 of purple photosynthetic bacterium Rhodobacter sphaeroides is blocked in the terminal hydrogenation steps of bacteriochlorophyll a biosynthesis. The mutant possesses only bacteriochlorophyll esterified with geranylgeraniol and has a reduced cellular level of the light-harvesting LH2 complex. Upon heterologous expression of the Synechocystis bchP homologue encoded by gene chlP, not only are hydrogenated forms of geranylgeranyl bacteriochlorophyllide a (bchlaGG) detectable, but the level of LH2 is increased, phenotype, overview	Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
44869	-	x * 44869, sequence calculation	Synechocystis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
bacteriochlorophyll a + 3 NADP+	Cereibacter sphaeroides	-	geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+	-	?
bacteriochlorophyll a + 3 NADP+	Synechocystis sp.	-	geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+	-	?
bacteriochlorophyll a + 3 NADP+	Cereibacter sphaeroides NCIB 8253	-	geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Cereibacter sphaeroides	Q9Z5D4	gene bchP	-
Cereibacter sphaeroides NCIB 8253	Q9Z5D4	gene bchP	-
Synechocystis sp.	Q55087	gene chlP; gene chlP	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
bacteriochlorophyll a + 3 NADP+	-	Cereibacter sphaeroides	geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+	-	?
bacteriochlorophyll a + 3 NADP+	-	Synechocystis sp.	geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+	-	?
bacteriochlorophyll a + 3 NADP+	-	Cereibacter sphaeroides NCIB 8253	geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+	-	?

Subunits

Subunits	Comment	Organism
?	x * 44869, sequence calculation	Synechocystis sp.

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Cereibacter sphaeroides
NADP+	-	Synechocystis sp.

General Information

General Information	Comment	Organism
malfunction	the bchP mutant T6G5 of purple photosynthetic bacterium Rhodobacter sphaeroides is blocked in the terminal hydrogenation steps of bacteriochlorophyll a biosynthesis. The mutant possesses only bacteriochlorophyll esterified with geranylgeraniol and has a reduced cellular level of the light-harvesting LH2 complex	Cereibacter sphaeroides
metabolism	the enzyme catalyzes the stepwise hydrogenation of geranylgeraniol to phytol during bacteriochlorophyll a biosynthesis	Synechocystis sp.
physiological function	the enzyme catalyzes the stepwise hydrogenation of geranylgeraniol to phytol during bacteriochlorophyll a biosynthesis	Synechocystis sp.