Data extracted from this reference:
Cloned(Commentary)
gene bchP, sequence comparison with gene chlP from Synechocystis sp. strain PCC 6803
Rhodobacter sphaeroides
gene chfP, DNA and amino acid sequence determination and analysis, the enzyme partially complements a bchP mutant T6G5 of purple photosynthetic bacterium Rhodobacter sphaeroides, which is blocked in the terminal hydrogenation steps of bacteriochlorophyll a biosynthesis. The mutant possesses only bacteriochlorophyll esterified with geranylgeraniol and has a reduced cellular level of the light-harvesting LH2 complex. Upon heterologous expression of the Synechocystis bchP homologue, not only are hydrogenated forms of geranylgeranyl bacteriochlorophyllide a (bchlaGG) detectable, but the level of LH2 is increased, sequence comparison with gene chP from Rhodobacter sphaeroides strain NCIB 8253
Synechocystis sp.
Engineering
additional information
the bchP mutant T6G5 of purple photosynthetic bacterium Rhodobacter sphaeroides is blocked in the terminal hydrogenation steps of bacteriochlorophyll a biosynthesis. The mutant possesses only bacteriochlorophyll esterified with geranylgeraniol and has a reduced cellular level of the light-harvesting LH2 complex. Upon heterologous expression of the Synechocystis bchP homologue encoded by gene chlP, not only are hydrogenated forms of geranylgeranyl bacteriochlorophyllide a (bchlaGG) detectable, but the level of LH2 is increased, phenotype, overview
Rhodobacter sphaeroides
Molecular Weight [Da]
44869
x * 44869, sequence calculation
Synechocystis sp.
Natural Substrates/ Products (Substrates)
bacteriochlorophyll a + 3 NADP+
Rhodobacter sphaeroides
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
?
bacteriochlorophyll a + 3 NADP+
Synechocystis sp.
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
?
bacteriochlorophyll a + 3 NADP+
Rhodobacter sphaeroides NCIB 8253
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
?
Organism
Rhodobacter sphaeroides
Q9Z5D4
gene bchP
Rhodobacter sphaeroides NCIB 8253
Q9Z5D4
gene bchP
Synechocystis sp.
Q55087
gene chlP; gene chlP
Substrates and Products (Substrate)
bacteriochlorophyll a + 3 NADP+
736098
Rhodobacter sphaeroides
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
?
bacteriochlorophyll a + 3 NADP+
736098
Synechocystis sp.
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
?
bacteriochlorophyll a + 3 NADP+
736098
Rhodobacter sphaeroides NCIB 8253
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
?
Subunits
?
x * 44869, sequence calculation
Synechocystis sp.
Cofactor
NADP+
Rhodobacter sphaeroides
Cloned(Commentary) (protein specific)
gene bchP, sequence comparison with gene chlP from Synechocystis sp. strain PCC 6803
Rhodobacter sphaeroides
gene chfP, DNA and amino acid sequence determination and analysis, the enzyme partially complements a bchP mutant T6G5 of purple photosynthetic bacterium Rhodobacter sphaeroides, which is blocked in the terminal hydrogenation steps of bacteriochlorophyll a biosynthesis. The mutant possesses only bacteriochlorophyll esterified with geranylgeraniol and has a reduced cellular level of the light-harvesting LH2 complex. Upon heterologous expression of the Synechocystis bchP homologue, not only are hydrogenated forms of geranylgeranyl bacteriochlorophyllide a (bchlaGG) detectable, but the level of LH2 is increased, sequence comparison with gene chP from Rhodobacter sphaeroides strain NCIB 8253
Synechocystis sp.
Cofactor (protein specific)
NADP+
Rhodobacter sphaeroides
Engineering (protein specific)
additional information
the bchP mutant T6G5 of purple photosynthetic bacterium Rhodobacter sphaeroides is blocked in the terminal hydrogenation steps of bacteriochlorophyll a biosynthesis. The mutant possesses only bacteriochlorophyll esterified with geranylgeraniol and has a reduced cellular level of the light-harvesting LH2 complex. Upon heterologous expression of the Synechocystis bchP homologue encoded by gene chlP, not only are hydrogenated forms of geranylgeranyl bacteriochlorophyllide a (bchlaGG) detectable, but the level of LH2 is increased, phenotype, overview
Rhodobacter sphaeroides
Molecular Weight [Da] (protein specific)
44869
x * 44869, sequence calculation
Synechocystis sp.
Natural Substrates/ Products (Substrates) (protein specific)
bacteriochlorophyll a + 3 NADP+
Rhodobacter sphaeroides
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
?
bacteriochlorophyll a + 3 NADP+
Synechocystis sp.
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
?
bacteriochlorophyll a + 3 NADP+
Rhodobacter sphaeroides NCIB 8253
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
?
Substrates and Products (Substrate) (protein specific)
bacteriochlorophyll a + 3 NADP+
736098
Rhodobacter sphaeroides
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
?
bacteriochlorophyll a + 3 NADP+
736098
Synechocystis sp.
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
?
bacteriochlorophyll a + 3 NADP+
736098
Rhodobacter sphaeroides NCIB 8253
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
?
Subunits (protein specific)
?
x * 44869, sequence calculation
Synechocystis sp.
General Information
malfunction
the bchP mutant T6G5 of purple photosynthetic bacterium Rhodobacter sphaeroides is blocked in the terminal hydrogenation steps of bacteriochlorophyll a biosynthesis. The mutant possesses only bacteriochlorophyll esterified with geranylgeraniol and has a reduced cellular level of the light-harvesting LH2 complex
Rhodobacter sphaeroides
metabolism
the enzyme catalyzes the stepwise hydrogenation of geranylgeraniol to phytol during bacteriochlorophyll a biosynthesis
Synechocystis sp.
physiological function
the enzyme catalyzes the stepwise hydrogenation of geranylgeraniol to phytol during bacteriochlorophyll a biosynthesis
Synechocystis sp.
General Information (protein specific)
malfunction
the bchP mutant T6G5 of purple photosynthetic bacterium Rhodobacter sphaeroides is blocked in the terminal hydrogenation steps of bacteriochlorophyll a biosynthesis. The mutant possesses only bacteriochlorophyll esterified with geranylgeraniol and has a reduced cellular level of the light-harvesting LH2 complex
Rhodobacter sphaeroides
metabolism
the enzyme catalyzes the stepwise hydrogenation of geranylgeraniol to phytol during bacteriochlorophyll a biosynthesis
Synechocystis sp.
physiological function
the enzyme catalyzes the stepwise hydrogenation of geranylgeraniol to phytol during bacteriochlorophyll a biosynthesis
Synechocystis sp.
Other publictions for EC
736948
Harada
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2008
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1
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2
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692822
Addlesee
Rhodospirillum rubrum possesse ...
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J. Bacteriol.
184
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2002
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3
3
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692821
Addlesee
Physical mapping and functiona ...
Rhodobacter sphaeroides
J. Bacteriol.
181
7248-7255
1999
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3
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736098
Addlesee
Cloning, sequencing and functi ...
Rhodobacter sphaeroides, Rhodobacter sphaeroides NCIB 8253, Synechocystis sp.
FEBS Lett.
389
126-130
1996
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2
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1
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3
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3
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1
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3
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735661
Bollivar
Molecular genetic analysis of ...
Rhodobacter capsulatus, Rhodobacter capsulatus SB1003
Biochemistry
33
12763-12768
1994
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