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Literature summary for 1.3.1.111 extracted from

  • Addlesee, H.A.; Hunter, C.N.
    Rhodospirillum rubrum possesses a variant of the bchP gene, encoding geranylgeranyl-bacteriopheophytin reductase (2002), J. Bacteriol., 184, 1578-1586.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.111

Cloned(Commentary)

Cloned (Comment) Organism
gene bchP, DNA and amino acid sequence determination and analysis, sequence comparison Rhodospirillum rubrum

Protein Variants

Protein Variants Comment Organism
additional information construction of a a hybrid gene consisting of the 5'half of Rhodospirillum rubrum bchP and the 3' half of Rhodobacter sphaeroides bchP, with the fragments fused as for bchPDELTAx, possesses neither geranylgeranyl-bacteriopheophytin reductase nor geranylgeranyl-bacteriochlorophyll a reductase activity. When complenmentation of the mutant is attempted with a similar fusion between the 5' half of Rhodobacter sphaeroides bchP and the 3' half of Rhodospirillum rubrum bchP, expressed in plasmid pSK1bchPS/PR, not only is BpheaP biosynthesis restored, but approximately 10% of the Bchl present is found to be esterified with dihydro-GG. Complete restoration of both BchlaP and BpheaP synthesis is achieved by using the positive control plasmid, pSK1bchPS/PS, in which the two relevant Rhodobacter sphaeroides bchP fragments are reunited Cereibacter sphaeroides
additional information construction of a hybrid gene consisting of the 5' half of Rhodospirillum rubrum bchP and the 3' half of Rhodobacter sphaeroides bchP, with the fragments fused as for bchPDELTAx, possesses neither geranylgeranyl-bacteriopheophytin reductase nor geranylgeranyl-bacteriochlorophyll a reductase activity. When complenmentation of the mutant is attempted with a similar fusion between the 5' half of Rhodobacter sphaeroides bchP and the 3' half of Rhodospirillum rubrum bchP, expressed in plasmid pSK1bchPS/PR, not only is BpheaP biosynthesis restored, but approximately 10% of the Bchl present is found to be esterified with dihydro-GG. Complete restoration of both BchlaP and BpheaP synthesis is achieved by using the positive control plasmid, pSK1bchPS/PS, in which the two relevant Rhodobacter sphaeroides bchP fragments are reunited Rhodospirillum rubrum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
bacteriochlorophyll a + 3 NADP+ Cereibacter sphaeroides
-
 geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
 ?
bacteriopheophytin a + 3 NADP+ Rhodospirillum rubrum
-
 geranylgeranyl bacteriopheophytin a + 3 NADPH + 3 H+
-
 ?
bacteriopheophytin a + 3 NADP+ Cereibacter sphaeroides
-
 geranylgeranyl bacteriopheophytin a + 3 NADPH + 3 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides Q9Z5D4
-
-
Cereibacter sphaeroides Q9Z5D4 gene bchP
-
Rhodospirillum rubrum Q936J5
-
-
Rhodospirillum rubrum Q936J5 gene bchP
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bacteriochlorophyll a + 3 NADP+
-
 Cereibacter sphaeroides geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
 ?
bacteriopheophytin a + 3 NADP+
-
 Rhodospirillum rubrum geranylgeranyl bacteriopheophytin a + 3 NADPH + 3 H+
-
 ?
bacteriopheophytin a + 3 NADP+
-
 Cereibacter sphaeroides geranylgeranyl bacteriopheophytin a + 3 NADPH + 3 H+
-
 ?
geranylgeranyl-bacteriochlorophyll a + 3 NADPH + 3 H+
-
 Rhodospirillum rubrum phytyl-bacteriochlorophyll a + 3 NADP+
-
 ?
geranylgeranyl-bacteriochlorophyll a + 3 NADPH + 3 H+
-
 Cereibacter sphaeroides phytyl-bacteriochlorophyll a + 3 NADP+
-
 ?
geranylgeranyl-bacteriopheophytin a + NADPH + H+
-
 Cereibacter sphaeroides phytyl-bacteriopheophytin a + NADP+
-
 ?
additional information no activity with geranylgeranyl-bacteriopheophytin a Rhodospirillum rubrum ?
-
 ?
additional information no activity with bacteriochlorophyll a Rhodospirillum rubrum ?
-
 ?

Synonyms

Synonyms Comment Organism
BchP
-
 Rhodospirillum rubrum
BchP
-
 Cereibacter sphaeroides
geranylgeranyl-bacteriopheophytin reductase
-
 Rhodospirillum rubrum
geranylgeranyl-bacteriopheophytin reductase
-
 Cereibacter sphaeroides
GG-Bphe reductase
-
 Rhodospirillum rubrum
GG-Bphe reductase
-
 Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Rhodospirillum rubrum
NADP+
-
 Cereibacter sphaeroides
NADPH
-
 Rhodospirillum rubrum
NADPH
-
 Cereibacter sphaeroides

General Information

General Information Comment Organism
malfunction the enzyme only catalyzes the reduction of the isoprenoid moiety of bacteriopheophytin. It might be a naturally occurring bchP mutant with an insertion mutation that may have been the initial cause of a partial loss of function Rhodospirillum rubrum
metabolism requirement of Rhodospirillum rubrum for phytylated bacteriopheophytin, a potential link between the absence of light-harvesting complex 2 and of phytylated bacteriochlorophyll from the wild-type bacterium. In addition to bacteriochlorophyll, the reaction center of purple bacteria contains two bacteriopheophytin molecules, one of which is the first clearly resolved acceptor of electrons, following electron transfer from the bacteriochlorophyll dimer. Proposed pathway for reduction of geranylgeranyl bacteriopheophytin a in Rhodospirillum rubrum, reduction proceeds via dihydro-GG-esterified and tetrahydro-GG-esterified intermediates to the final product, phytylated bacteriopheophytin Rhodospirillum rubrum
physiological function the enzyme is responsible for the reduction of the isoprenoid moiety of bacteriochlorophyll (Bchl) from geranylgeraniol (GG) to phytol, it also catalyzes the reduction of the isoprenoid moiety of bacteriopheophytin Cereibacter sphaeroides