BRENDA - Enzyme Database
show all sequences of 1.3.1.111

Physical mapping and functional assignment of the geranylgeranyl-bacteriochlorophyll reductase gene, bchP, of Rhodobacter sphaeroides

Addlesee, H.A.; Hunter, C.N.; J. Bacteriol. 181, 7248-7255 (1999)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli; gene bchP, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS. The recombinant enzyme complements the bchP insertion mutant T6G5
Rhodobacter sphaeroides
Engineering
Amino acid exchange
Commentary
Organism
additional information
a transposon mutant of gene bchP encoding geranylgeranyl-bacteriochlorophyll reductase possesses a structurally modified photosystem assembled with bacteriochlorophyll esterified with geranylgeraniol, rather than with phytol
Rhodobacter sphaeroides
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
bacteriochlorophyll a + 3 NADP+
Rhodobacter sphaeroides
-
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
-
?
geranylgeranyl-bacteriochlorophyll a + 3 NADPH + 3 H+
Rhodobacter sphaeroides
the enzyme is involved in phythylation of bacteriochlorophyll a
phytyl-bacteriochlorophyll a + 3 NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodobacter sphaeroides
Q9Z5D4
; gene bchP
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
bacteriochlorophyll a + 3 NADP+
-
692821
Rhodobacter sphaeroides
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
-
-
?
bacteriochlorophyll a + 3 NADP+
activity assay is performed in the dark
692821
Rhodobacter sphaeroides
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
-
-
?
geranylgeranyl-bacteriochlorophyll a + 3 NADPH + 3 H+
-
692821
Rhodobacter sphaeroides
phytyl-bacteriochlorophyll a + 3 NADP+
-
-
-
?
geranylgeranyl-bacteriochlorophyll a + 3 NADPH + 3 H+
the enzyme is involved in phythylation of bacteriochlorophyll a
692821
Rhodobacter sphaeroides
phytyl-bacteriochlorophyll a + 3 NADP+
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Rhodobacter sphaeroides
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Rhodobacter sphaeroides
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Rhodobacter sphaeroides
NADPH
-
Rhodobacter sphaeroides
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli; gene bchP, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS. The recombinant enzyme complements the bchP insertion mutant T6G5
Rhodobacter sphaeroides
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Rhodobacter sphaeroides
NADPH
-
Rhodobacter sphaeroides
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
a transposon mutant of gene bchP encoding geranylgeranyl-bacteriochlorophyll reductase possesses a structurally modified photosystem assembled with bacteriochlorophyll esterified with geranylgeraniol, rather than with phytol
Rhodobacter sphaeroides
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
bacteriochlorophyll a + 3 NADP+
Rhodobacter sphaeroides
-
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
-
?
geranylgeranyl-bacteriochlorophyll a + 3 NADPH + 3 H+
Rhodobacter sphaeroides
the enzyme is involved in phythylation of bacteriochlorophyll a
phytyl-bacteriochlorophyll a + 3 NADP+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
bacteriochlorophyll a + 3 NADP+
-
692821
Rhodobacter sphaeroides
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
-
-
?
bacteriochlorophyll a + 3 NADP+
activity assay is performed in the dark
692821
Rhodobacter sphaeroides
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
-
-
?
geranylgeranyl-bacteriochlorophyll a + 3 NADPH + 3 H+
-
692821
Rhodobacter sphaeroides
phytyl-bacteriochlorophyll a + 3 NADP+
-
-
-
?
geranylgeranyl-bacteriochlorophyll a + 3 NADPH + 3 H+
the enzyme is involved in phythylation of bacteriochlorophyll a
692821
Rhodobacter sphaeroides
phytyl-bacteriochlorophyll a + 3 NADP+
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Rhodobacter sphaeroides
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Rhodobacter sphaeroides
General Information
General Information
Commentary
Organism
malfunction
a transposon mutant of gene bchP encoding geranylgeranyl-bacteriochlorophyll reductase possesses a structurally modified photosystem assembled with bacteriochlorophyll esterified with geranylgeraniol, rather than with phytol
Rhodobacter sphaeroides
metabolism
the enzyme catalyzes the stepwise hydrogenation of geranylgeraniol to phytol during bacteriochlorophyll a biosynthesis, pathway for the phytylation of bacteriochlorophyll a, overview
Rhodobacter sphaeroides
physiological function
the bacteriochlorophyll of the purple photosynthetic bacterium Rhodobacter sphaeroides is esterified with phytol. The presence of this alcohol moiety is essential for the correct assembly of the photosynthetic apparatus. The enzyme is essential and responsible for the reduction of the alcohol moiety of (bacterio)chlorophyll to phytol
Rhodobacter sphaeroides
General Information (protein specific)
General Information
Commentary
Organism
malfunction
a transposon mutant of gene bchP encoding geranylgeranyl-bacteriochlorophyll reductase possesses a structurally modified photosystem assembled with bacteriochlorophyll esterified with geranylgeraniol, rather than with phytol
Rhodobacter sphaeroides
metabolism
the enzyme catalyzes the stepwise hydrogenation of geranylgeraniol to phytol during bacteriochlorophyll a biosynthesis, pathway for the phytylation of bacteriochlorophyll a, overview
Rhodobacter sphaeroides
physiological function
the bacteriochlorophyll of the purple photosynthetic bacterium Rhodobacter sphaeroides is esterified with phytol. The presence of this alcohol moiety is essential for the correct assembly of the photosynthetic apparatus. The enzyme is essential and responsible for the reduction of the alcohol moiety of (bacterio)chlorophyll to phytol
Rhodobacter sphaeroides
Other publictions for EC 1.3.1.111
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736948
Harada
Accumulation of chlorophyllous ...
Chlorobaculum tepidum, Chlorobaculum tepidum WT2321
Photochem. Photobiol. Sci.
7
1179-1187
2008
-
-
1
-
1
-
-
1
-
-
-
2
-
2
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-
-
-
-
-
2
-
-
-
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-
1
-
-
1
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-
1
1
-
1
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-
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-
1
-
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-
2
-
-
-
-
-
-
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-
2
-
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-
-
-
1
-
-
-
-
2
2
-
-
-
692822
Addlesee
Rhodospirillum rubrum possesse ...
Rhodobacter sphaeroides, Rhodospirillum rubrum
J. Bacteriol.
184
1578-1586
2002
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-
1
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2
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-
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-
-
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3
-
6
-
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-
-
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-
8
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-
-
-
-
-
-
4
-
-
-
-
-
1
4
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
692821
Addlesee
Physical mapping and functiona ...
Rhodobacter sphaeroides
J. Bacteriol.
181
7248-7255
1999
-
-
1
-
1
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
3
3
-
-
-
736098
Addlesee
Cloning, sequencing and functi ...
Rhodobacter sphaeroides, Rhodobacter sphaeroides NCIB 8253, Synechocystis sp.
FEBS Lett.
389
126-130
1996
-
-
2
-
1
-
-
-
-
-
1
3
-
3
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
2
-
-
-
-
-
2
2
-
1
-
-
-
-
-
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-
1
3
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
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3
3
-
-
-
735661
Bollivar
Molecular genetic analysis of ...
Rhodobacter capsulatus, Rhodobacter capsulatus SB1003
Biochemistry
33
12763-12768
1994
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1
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1
-
-
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-
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2
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2
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-
-
-
-
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4
-
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-
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-
1
-
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-
1
1
-
1
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2
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4
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-
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2
2
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