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Literature summary for 1.3.1.105 extracted from

  • Klein, D.; Fink, B.; Arold, B.; Eisenreich, W.; Schwab, W.
    Functional characterization of enone oxidoreductases from strawberry and tomato fruit (2007), J. Agric. Food Chem., 55, 6705-6711.
    View publication on PubMed
Search for more literature for 1.3.1.105

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli K-12 Solanum lycopersicum
expressed in Escherichia coli K-12 Fragaria x ananassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 9,10-phenanthrenequinone 0.1 M Na2HPO4/NaH2PO4, pH 7, 30°C, cofactor NADH Fragaria x ananassa
0.82
-
 (2E)-2-butylidene-4-hydroxy-5-methyl-3(2H)-furanone 0.1 M Na2HPO4/NaH2PO4, pH 7, 30°C, cofactor NADH Fragaria x ananassa
1.04
-
 (2E)-4-hydroxy-5-methyl-2-propylidene-3(2H)-furanone 0.1 M Na2HPO4/NaH2PO4, pH 7, 30°C, cofactor NADH Fragaria x ananassa
1.13
-
 (2E)-2-butylidene-4-hydroxy-5-methyl-3(2H)-furanone 0.1 M Na2HPO4/NaH2PO4, pH 7, 30°C, cofactor NADH Solanum lycopersicum
1.59
-
 (2E)-ethylidene-4-hydroxy-5-methyl-3(2H)-furanone 0.1 M Na2HPO4/NaH2PO4, pH 7, 30°C, cofactor NADH Solanum lycopersicum
2.14
-
 (2E)-ethylidene-4-hydroxy-5-methyl-3(2H)-furanone 0.1 M Na2HPO4/NaH2PO4, pH 7, 30°C, cofactor NADH Fragaria x ananassa
2.41
-
 (2E)-4-hydroxy-5-methyl-2-propylidene-3(2H)-furanone 0.1 M Na2HPO4/NaH2PO4, pH 7, 30°C, cofactor NADH Solanum lycopersicum

Organism

Organism UniProt Comment Textmining
Fragaria x ananassa Q941I0
-
-
Solanum lycopersicum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Solanum lycopersicum
-
fruit
-
 Fragaria x ananassa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E)-2-butylidene-4-hydroxy-5-methyl-3(2H)-furanone + NADH + H+
-
 Solanum lycopersicum 2-butyl-4-hydroxy-5-methyl-3(2H)-furanone + NAD+
-
 ?
(2E)-2-butylidene-4-hydroxy-5-methyl-3(2H)-furanone + NADH + H+
-
 Fragaria x ananassa 2-butyl-4-hydroxy-5-methyl-3(2H)-furanone + NAD+
-
 ?
(2E)-4-hydroxy-5-methyl-2-propylidene-3(2H)-furanone + NADH + H+
-
 Solanum lycopersicum 4-hydroxy-5-methyl-2-propyl-3(2H)-furanone + NAD+
-
 ?
(2E)-4-hydroxy-5-methyl-2-propylidene-3(2H)-furanone + NADH + H+
-
 Fragaria x ananassa 4-hydroxy-5-methyl-2-propyl-3(2H)-furanone + NAD+
-
 ?
(2E)-ethylidene-4-hydroxy-5-methyl-3(2H)-furanone + NADH + H+
-
 Solanum lycopersicum 2-ethyl-4-hydroxy-5-methyl-3(2H)-furanone + NAD+
-
 ?
(2E)-ethylidene-4-hydroxy-5-methyl-3(2H)-furanone + NADH + H+
-
 Fragaria x ananassa 2-ethyl-4-hydroxy-5-methyl-3(2H)-furanone + NAD+
-
 ?
4-hydroxy-5-methyl-2-methylene-3(2H)-furanone + NADH + H+
-
 Solanum lycopersicum 4-hydroxy-2,5-dimethyl-3(2H)-furanone + NAD+
-
 ?
4-hydroxy-5-methyl-2-methylene-3(2H)-furanone + NADH + H+
-
 Fragaria x ananassa 4-hydroxy-2,5-dimethyl-3(2H)-furanone + NAD+
-
 ?
9,10-phenanthrenequinone + NADH + H+
-
 Fragaria x ananassa ? + NAD+
-
 ?
additional information no reaction with (2E)-2-hexenal, (3E)-3-hexen-2-one, (3E)-5-methyl-3-hexen-2-one, cyclohexa-1,2-dione, buta-2,3-dione, coumarin, astaxanthin, cinnamyl aldehyde, 4-coumaric acid, ferulic acid, dimethoxycinnamic acid, or benzil Solanum lycopersicum ?
-
 ?
additional information no reaction with (2E)-2-hexenal, (3E)-3-hexen-2-one, (3E)-5-methyl-3-hexen-2-one, cyclohexa-1,2-dione, buta-2,3-dione, coumarin, astaxanthin, cinnamyl aldehyde, 4-coumaric acid, ferulic acid, dimethoxycinnamic acid, or benzil Fragaria x ananassa ?
-
 ?

Synonyms

Synonyms Comment Organism
FaEO
-
 Fragaria x ananassa
SIEO
-
 Solanum lycopersicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.15
-
 (2E)-2-butylidene-4-hydroxy-5-methyl-3(2H)-furanone 0.1 M Na2HPO4/NaH2PO4, pH 7, 30°C, cofactor NADH Solanum lycopersicum
0.34
-
 (2E)-4-hydroxy-5-methyl-2-propylidene-3(2H)-furanone 0.1 M Na2HPO4/NaH2PO4, pH 7, 30°C, cofactor NADH Solanum lycopersicum
0.39
-
 (2E)-ethylidene-4-hydroxy-5-methyl-3(2H)-furanone 0.1 M Na2HPO4/NaH2PO4, pH 7, 30°C, cofactor NADH Solanum lycopersicum
1.43
-
 (2E)-2-butylidene-4-hydroxy-5-methyl-3(2H)-furanone 0.1 M Na2HPO4/NaH2PO4, pH 7, 30°C, cofactor NADH Fragaria x ananassa
1.94
-
 (2E)-ethylidene-4-hydroxy-5-methyl-3(2H)-furanone 0.1 M Na2HPO4/NaH2PO4, pH 7, 30°C, cofactor NADH Fragaria x ananassa
2.69
-
 (2E)-4-hydroxy-5-methyl-2-propylidene-3(2H)-furanone 0.1 M Na2HPO4/NaH2PO4, pH 7, 30°C, cofactor NADH Fragaria x ananassa

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Solanum lycopersicum
NADH
-
 Fragaria x ananassa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.14
-
 (2E)-4-hydroxy-5-methyl-2-propylidene-3(2H)-furanone 0.1 M Na2HPO4/NaH2PO4, pH 7, 30°C, cofactor NADH Solanum lycopersicum
0.14
-
 (2E)-2-butylidene-4-hydroxy-5-methyl-3(2H)-furanone 0.1 M Na2HPO4/NaH2PO4, pH 7, 30°C, cofactor NADH Solanum lycopersicum
0.25
-
 (2E)-ethylidene-4-hydroxy-5-methyl-3(2H)-furanone 0.1 M Na2HPO4/NaH2PO4, pH 7, 30°C, cofactor NADH Solanum lycopersicum
0.9
-
 (2E)-ethylidene-4-hydroxy-5-methyl-3(2H)-furanone 0.1 M Na2HPO4/NaH2PO4, pH 7, 30°C, cofactor NADH Fragaria x ananassa
1.9
-
 (2E)-2-butylidene-4-hydroxy-5-methyl-3(2H)-furanone 0.1 M Na2HPO4/NaH2PO4, pH 7, 30°C, cofactor NADH Fragaria x ananassa
2.6
-
 (2E)-4-hydroxy-5-methyl-2-propylidene-3(2H)-furanone 0.1 M Na2HPO4/NaH2PO4, pH 7, 30°C, cofactor NADH Fragaria x ananassa
650
-
 9,10-phenanthrenequinone 0.1 M Na2HPO4/NaH2PO4, pH 7, 30°C, cofactor NADH Fragaria x ananassa