Literature summary for 1.3.1.104 extracted from

Khan, R.; Zeb, A.; Roy, N.; Thapa Magar, R.; Kim, H.J.; Lee, K.W.; Lee, S.W.
Biochemical and structural basis of triclosan resistance in a novel enoyl-acyl carrier protein reductase (2018), Antimicrob. Agents Chemother., 62, e00648-18 .

Search for more literature for 1.3.1.104

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli mutant JPP1111	uncultured bacterium pBF1

General Stability

General Stability	Organism
no enzymatic activity is observed in 100 mM sodium citrate buffer regardless of the pH	uncultured bacterium pBF1

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	the enzyme is resistant to triclosan	uncultured bacterium pBF1

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.009627	-	crotonyl-CoA	at pH 7.0 and 25°C	uncultured bacterium pBF1
0.02764	-	NADPH	at pH 7.0 and 25°C	uncultured bacterium pBF1

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
crotonyl-[acyl-carrier-protein] + NADPH + H+	uncultured bacterium pBF1	-	butyryl-[acyl-carrier-protein] + NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
uncultured bacterium pBF1	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
crotonyl-CoA + NADPH + H+	-	uncultured bacterium pBF1	butyryl-CoA + NADP+	-	?
crotonyl-[acyl-carrier-protein] + NADPH + H+	-	uncultured bacterium pBF1	butyryl-[acyl-carrier-protein] + NADP+	-	?
additional information	the enzyme shows no 7-alpha hydroxysteroid dehydrogenase activity when tested with cholic acid substrate and NADH and NADPH cofactors. The purified protein does not utilize NADH as a cofactor when tested with crotonyl-[CoA] as substrate	uncultured bacterium pBF1	?	-	-

Subunits

Subunits	Comment	Organism
?	x * 33560, calculated from amino acid sequence	uncultured bacterium pBF1

Synonyms

Synonyms	Comment	Organism
enoyl-ACP reductase	-	uncultured bacterium pBF1
enoyl-acyl carrier protein reductase	-	uncultured bacterium pBF1
ENR	-	uncultured bacterium pBF1
Fabl2	-	uncultured bacterium pBF1

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.009627	-	crotonyl-CoA	at pH 7.0 and 25°C	uncultured bacterium pBF1
0.02764	-	NADPH	at pH 7.0 and 25°C	uncultured bacterium pBF1

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	in 100 mM sodium phosphate buffer	uncultured bacterium pBF1

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	uncultured bacterium pBF1
FMN	-	uncultured bacterium pBF1
NADPH	-	uncultured bacterium pBF1

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Release 2023.1 (January 2023)