Cloned (Comment) | Organism |
---|---|
gene aor, real-time PCR. enzyme expression analysis | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of AtAOR (aor) mutants, including a transfer DNA knockout, aor (T-DNA), and RNA interference knockdown, aor (RNAi), lines. Both aor mutants show smaller plant sizes than wild-type plants when they are grown under day/night cycle conditions | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acrolein + NADPH + H+ | Arabidopsis thaliana | - |
? + NADP+ | - |
? | |
additional information | Arabidopsis thaliana | enzyme AOR shows acrolein-reducing activity in addition to the cytosolic acrolein detoxification enzyme in leaves of higher plants | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9ZUC1 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acrolein + NADPH + H+ | - |
Arabidopsis thaliana | ? + NADP+ | - |
? | |
additional information | enzyme AOR shows acrolein-reducing activity in addition to the cytosolic acrolein detoxification enzyme in leaves of higher plants | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, about, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
AOR | - |
Arabidopsis thaliana |
AtAOR | - |
Arabidopsis thaliana |
chloroplastic alkenal/one oxidoreductase | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | suppression of chloroplastic alkenal/one oxidoreductase represses the carbon catabolic pathway in Arabidopsis leaves during night. Phosphoenolpyruvate carboxylase activity decreases and starch degradation during the night is suppressed in aor (RNAi). In contrast, the phenotype of aor (RNAi) is rescued when aor (RNAi) plants are grown under constant light conditions. The smaller plant sizes observed in aor mutants grown under day/night cycle conditions are attributable to the decrease in carbon utilization during the night. The acrolein reducing activity decreases to about 60% and 75%in aor-1 and aor-4 mutant lines, respectively, compared with the wild-type. Lack of AtAOR in chloroplasts induces oxidative stress. Phenotype analysis, detailed overview | Arabidopsis thaliana |
physiological function | lipid-derived reactive carbonyl species (RCS) possess electrophilic moieties and cause oxidative stress by reacting with cellular components. Arabidopsis thaliana has a chloroplast-localized alkenal/one oxidoreductase (AtAOR) for the detoxification of lipid-derived RCS, especially alpha,beta-unsaturated carbonyls. The detoxification of lipid-derived RCS by AtAOR in chloroplasts contributes to the protection of dark respiration and supports plant growth during the night | Arabidopsis thaliana |