Cloned (Comment) | Organism |
---|---|
NtRed-1 cloned in Escherichia coli | Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
(R)-pulegone | recombinant NtRed-1, pH 7.0 and 35°C | Nicotiana tabacum | |
8.3 | - |
(S)-pulegone | recombinant NtRed-1, pH 7.0 and 35°C | Nicotiana tabacum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36300 | - |
native NtRed-1, SDS-PAGE | Nicotiana tabacum |
38000 | - |
recombinant NtRed-1, 2 * 38000, gel filtration chromatography | Nicotiana tabacum |
38480 | - |
recombinant NtRed-1, MALDI-TOF MS spectra | Nicotiana tabacum |
74000 | - |
recombinant NtRed-1, gel filtration chromatography | Nicotiana tabacum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | Q9SLN8 | - |
- |
Purification (Comment) | Organism |
---|---|
by DEAEToyopearl and RedToyopearl column chromatography | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-pulegone + NADPH + H+ | - |
Nicotiana tabacum | (1R,4R)-isomenthone + (1R,4S)-menthone + NADP+ | - |
? | |
(S)-pulegone + NADPH + H+ | - |
Nicotiana tabacum | (1S,4S)-isomenthone + (1S,4R)-menthone + NADP+ | - |
? | |
additional information | recombinant NtRed-1 does not catalyze dehydrogenation of (2S,4S)-carveol, indicating that the recombinant NtRed-1 lacks the catalytic function as allyl-alcohol dehydrogenase, although the recombinant NtRed-1 is designed from the nucleotide sequence of allyl-ADH | Nicotiana tabacum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | recombinant NtRed-1, 2 * 38000, gel filtration chromatography | Nicotiana tabacum |
Synonyms | Comment | Organism |
---|---|---|
allyl-alcohol dehydrogenase | - |
Nicotiana tabacum |
NtRed-1 | - |
Nicotiana tabacum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
(S)-pulegone | recombinant NtRed-1, pH 7.0 and 35°C | Nicotiana tabacum | |
3.3 | - |
(R)-pulegone | recombinant NtRed-1, pH 7.0 and 35°C | Nicotiana tabacum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
(S)-pulegone | recombinant NtRed-1, pH 7.0 and 35°C | Nicotiana tabacum | |
2.4 | - |
(R)-pulegone | recombinant NtRed-1, pH 7.0 and 35°C | Nicotiana tabacum |