BRENDA - Enzyme Database
show all sequences of 1.3.1.100

Ergot alkaloid biosynthesis in Aspergillus fumigatus: Conversion of chanoclavine-I aldehyde to festuclavine by the festuclavine synthase FgaFS in the presence of the old yellow enzyme FgaOx3

Wallwey, C.; Matuschek, M.; Xie, X.; Li, S.; Org. Biomol. Chem. 8, 3500-3508 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli XL1 Blue MRF’ cells
Aspergillus fumigatus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
1 * 45000, SDS-PAGE
Aspergillus fumigatus
46000
-
gel filtration
Aspergillus fumigatus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus fumigatus
V5IVA1
-
-
Aspergillus fumigatus B5233
V5IVA1
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA agarose column chromatography
Aspergillus fumigatus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
chanoclavine-I aldehyde + NADH + H+
in the presence of FgaFS only
720615
Aspergillus fumigatus
dihydrochanoclavine-I aldehyde + NAD+
-
-
-
?
chanoclavine-I aldehyde + NADH + H+
in the presence of FgaFS only
720615
Aspergillus fumigatus B5233
dihydrochanoclavine-I aldehyde + NAD+
-
-
-
?
additional information
the enzyme is more active in the presence of FMN and NADH than other combinations, e.g. FMN and NADPH, FAD and NADH or FAD and NADPH
720615
Aspergillus fumigatus
?
-
-
-
-
additional information
the enzyme is more active in the presence of FMN and NADH than other combinations, e.g. FMN and NADPH, FAD and NADH or FAD and NADPH
720615
Aspergillus fumigatus B5233
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 45000, SDS-PAGE
Aspergillus fumigatus
Cofactor
Cofactor
Commentary
Organism
Structure
FMN
-
Aspergillus fumigatus
NADH
-
Aspergillus fumigatus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli XL1 Blue MRF’ cells
Aspergillus fumigatus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FMN
-
Aspergillus fumigatus
NADH
-
Aspergillus fumigatus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
1 * 45000, SDS-PAGE
Aspergillus fumigatus
46000
-
gel filtration
Aspergillus fumigatus
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA agarose column chromatography
Aspergillus fumigatus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
chanoclavine-I aldehyde + NADH + H+
in the presence of FgaFS only
720615
Aspergillus fumigatus
dihydrochanoclavine-I aldehyde + NAD+
-
-
-
?
chanoclavine-I aldehyde + NADH + H+
in the presence of FgaFS only
720615
Aspergillus fumigatus B5233
dihydrochanoclavine-I aldehyde + NAD+
-
-
-
?
additional information
the enzyme is more active in the presence of FMN and NADH than other combinations, e.g. FMN and NADPH, FAD and NADH or FAD and NADPH
720615
Aspergillus fumigatus
?
-
-
-
-
additional information
the enzyme is more active in the presence of FMN and NADH than other combinations, e.g. FMN and NADPH, FAD and NADH or FAD and NADPH
720615
Aspergillus fumigatus B5233
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 45000, SDS-PAGE
Aspergillus fumigatus
Other publictions for EC 1.3.1.100
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741523
Chilton
Structure of an Aspergillus f ...
Aspergillus fumigatus, Aspergillus fumigatus ATCC MYA-4609
Acta Crystallogr. Sect. F
70
1328-1332
2014
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1
1
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720361
Xie
Formyl migration product of ch ...
Aspergillus fumigatus
Magn. Reson. Chem.
49
678-681
2011
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1
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720615
Wallwey
Ergot alkaloid biosynthesis in ...
Aspergillus fumigatus, Aspergillus fumigatus B5233
Org. Biomol. Chem.
8
3500-3508
2010
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4
1
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721342
Coyle
An old yellow enzyme gene cont ...
Aspergillus fumigatus, Aspergillus fumigatus FGSC A1141
Appl. Environ. Microbiol.
76
3898-3903
2010
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2
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722448
Cheng
A role for old yellow enzyme i ...
Aspergillus fumigatus
J. Am. Chem. Soc.
132
1776-1777
2010
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