BRENDA - Enzyme Database show
show all sequences of 1.23.1.1

Pinoresinol reductase 1 impacts lignin distribution during secondary cell wall biosynthesis in Arabidopsis

Zhao, Q.; Zeng, Y.; Yin, Y.; Pu, Y.; Jackson, L.A.; Engle, N.L.; Martin, M.Z.; Tschaplinski, T.J.; Ding, S.Y.; Ragauskas, A.J.; Dixon, R.A.; Phytochemistry 112, 170-178 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene PrP2, gene co-expression networks for Arabidopsis thaliana PrR1 and PrR2, overview. PrR2 expression clusters with a different set of genes, not involved in secondary cell wall biosynthesis
Arabidopsis thaliana
Engineering
Amino acid exchange
Commentary
Organism
additional information
isozyme mutant prr2-1, SALK_123621
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(+)-pinoresinol + NADPH + H+
Arabidopsis thaliana
-
(+)-lariciresinol + NADP+
-
-
?
additional information
Arabidopsis thaliana
(+)-pinoresinol is successively converted into (-+)-lariciresinol and further into (-)-secoisolariciresinol by bifunctional enzyme PrR2, cf. EC 1.23.1.2
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q9SVP6
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
PrR2 expression is barely detectable in stems
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-pinoresinol + NADPH + H+
-
746001
Arabidopsis thaliana
(+)-lariciresinol + NADP+
-
-
-
?
additional information
(+)-pinoresinol is successively converted into (-+)-lariciresinol and further into (-)-secoisolariciresinol by bifunctional enzyme PrR2, cf. EC 1.23.1.2
746001
Arabidopsis thaliana
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
gene PrP2, gene co-expression networks for Arabidopsis thaliana PrR1 and PrR2, overview. PrR2 expression clusters with a different set of genes, not involved in secondary cell wall biosynthesis
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Arabidopsis thaliana
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
isozyme mutant prr2-1, SALK_123621
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(+)-pinoresinol + NADPH + H+
Arabidopsis thaliana
-
(+)-lariciresinol + NADP+
-
-
?
additional information
Arabidopsis thaliana
(+)-pinoresinol is successively converted into (-+)-lariciresinol and further into (-)-secoisolariciresinol by bifunctional enzyme PrR2, cf. EC 1.23.1.2
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
PrR2 expression is barely detectable in stems
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-pinoresinol + NADPH + H+
-
746001
Arabidopsis thaliana
(+)-lariciresinol + NADP+
-
-
-
?
additional information
(+)-pinoresinol is successively converted into (-+)-lariciresinol and further into (-)-secoisolariciresinol by bifunctional enzyme PrR2, cf. EC 1.23.1.2
746001
Arabidopsis thaliana
?
-
-
-
-
General Information
General Information
Commentary
Organism
metabolism
pinoresinol is successively converted into lariciresinol and secoisolariciresinol by PrR. Secoisolariciresinol is glycosylated to secoisolariciresinol diglucoside (SDG). PLR has enantiomeric control on the lignan biosynthetic pathway
Arabidopsis thaliana
physiological function
pinoresinol reductase catalyzes the conversion of the lignan pinoresinol to lariciresinol in Arabidopsis thaliana, where it is encoded by two genes, PrR1 and PrR2, that appear to act redundantly. The isozyme PrR2 is responsible for the synthesis of the enantiomer (-)-secoisolariciresinol
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
metabolism
pinoresinol is successively converted into lariciresinol and secoisolariciresinol by PrR. Secoisolariciresinol is glycosylated to secoisolariciresinol diglucoside (SDG). PLR has enantiomeric control on the lignan biosynthetic pathway
Arabidopsis thaliana
physiological function
pinoresinol reductase catalyzes the conversion of the lignan pinoresinol to lariciresinol in Arabidopsis thaliana, where it is encoded by two genes, PrR1 and PrR2, that appear to act redundantly. The isozyme PrR2 is responsible for the synthesis of the enantiomer (-)-secoisolariciresinol
Arabidopsis thaliana
Other publictions for EC 1.23.1.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746162
Corbin
Functional characterization o ...
Linum usitatissimum
Planta
246
405-420
2017
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2
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2
3
3
2
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-
746001
Zhao
Pinoresinol reductase 1 impac ...
Arabidopsis thaliana
Phytochemistry
112
170-178
2015
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2
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744143
Tamura
Successful expression of a no ...
Sphingobium sp. SYK-6
Appl. Microbiol. Biotechnol.
98
8165-8177
2014
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1
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1
1
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722146
Fukuhara
Discovery of pinoresinol reduc ...
Novosphingobium aromaticivorans, Novosphingobium aromaticivorans DSM 12444, Sphingobium sp.
Enzyme Microb. Technol.
52
38-43
2013
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723491
Hemmati
Pinoresinol-lariciresinol redu ...
Linum usitatissimum
Planta Med.
76
928-934
2010
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1
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722657
Nakatsubo
Characterization of Arabidopsi ...
Arabidopsis thaliana
J. Biol. Chem.
283
15550-15557
2008
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723420
Bayindir
Hinokinin biosynthesis in Linu ...
Linum corymbulosum
Plant J.
55
810-820
2008
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1
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717154
Vassao
A pinoresinol-lariciresinol re ...
Forsythia x intermedia
Arch. Biochem. Biophys.
465
209-218
2007
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722257
Hemmati
(+)-Pinoresinol/(-)-lariciresi ...
Linum perenne
FEBS Lett.
581
603-610
2007
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1
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1
1
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723223
Ayella
Enhancing lignan biosynthesis ...
Forsythia x intermedia
Mol. Nutr. Food Res.
51
1518-1526
2007
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1
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723360
von Heimendahl
Pinoresinol-lariciresinol redu ...
Linum album
Phytochemistry
66
1254-1263
2005
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1
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4
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722032
Xie
Biotransformation of pinoresin ...
Enterococcus faecalis, Enterococcus faecalis PDG-1
Chem. Pharm. Bull.
51
508-515
2003
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719799
Fujita
Recombinant pinoresinol-larici ...
Thuja plicata
J. Biol. Chem.
274
618-627
1999
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1
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3
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722601
Dinkova-Kostova
(+)-Pinoresinol/(+)-lariciresi ...
Forsythia x intermedia
J. Biol. Chem.
271
29473-29482
1996
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1
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719780
Chu
Stereospecificity of (+)-pinor ...
Forsythia x intermedia
J. Biol. Chem.
268
27026-27033
1993
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723354
Katayama
An extraordinary accumulation ...
Forsythia x intermedia
Phytochemistry
31
3875-3881
1992
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