Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.23.1.1 extracted from

  • Fukuhara, Y.; Kamimura, N.; Nakajima, M.; Hishiyama, S.; Hara, H.; Kasai, D.; Tsuji, Y.; Narita-Yamada, S.; Nakamura, S.; Katano, Y.; Fujita, N.; Katayama, Y.; Fukuda, M.; Kajita, S.; Masai, E.
    Discovery of pinoresinol reductase genes in sphingomonads (2013), Enzyme Microb. Technol., 52, 38-43.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Novosphingobium aromaticivorans
expressed in Escherichia coli BL21(DE3) cells Sphingobium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
gel filtration Sphingobium sp.
36000
-
1 * 36000, recombinant enzyme, SDS-PAGE Sphingobium sp.
36837
-
1 * 36837, calculated from amino acid sequence Sphingobium sp.

Organism

Organism UniProt Comment Textmining
Novosphingobium aromaticivorans
-
-
-
Novosphingobium aromaticivorans DSM 12444
-
-
-
Sphingobium sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Novosphingobium aromaticivorans
nickel affinity column chromatography Sphingobium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
1 micromol/mg/min specific activity towards (+/-)-lariciresinol in the presence of NADPH, at pH 7.0 and 30°C Sphingobium sp.
additional information
-
1 micromol/min/mg specific activity toward (+/-)-lariciresinol in the presence of NADPH, at pH 7.0 and 30°C Novosphingobium aromaticivorans
32
-
toward (+/-)-pinoresinol in the presence of NADPH, at pH 7.0 and 30°C Novosphingobium aromaticivorans
46
-
toward (+/-)-syringaresinol in the presence of NADPH, at pH 7.0 and 30°C Sphingobium sp.
61
-
toward (+/-)-syringaresinol in the presence of NADPH, at pH 7.0 and 30°C Novosphingobium aromaticivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+/-)-pinoresinol + NADPH + H+
-
Novosphingobium aromaticivorans (+/-)-lariciresinol + NADP+
-
?
(+/-)-pinoresinol + NADPH + H+
-
Sphingobium sp. (+/-)-lariciresinol + NADP+
-
?
(+/-)-pinoresinol + NADPH + H+
-
Novosphingobium aromaticivorans DSM 12444 (+/-)-lariciresinol + NADP+
-
?
(+/-)-syringaresinol + NADPH + H+
-
Novosphingobium aromaticivorans 5,5'-dimethoxylariciresinol + NADP+
-
?
(+/-)-syringaresinol + NADPH + H+
-
Sphingobium sp. 5,5'-dimethoxylariciresinol + NADP+
-
?
(+/-)-syringaresinol + NADPH + H+
-
Novosphingobium aromaticivorans DSM 12444 5,5'-dimethoxylariciresinol + NADP+
-
?
additional information negligible activity with (+/-)-lariciresinol compared to (+/-)-pinoresinol Novosphingobium aromaticivorans ?
-
?
additional information negligible activity with (+/-)-lariciresinol compared to (+/-)-pinoresinol Sphingobium sp. ?
-
?
additional information negligible activity with (+/-)-lariciresinol compared to (+/-)-pinoresinol Novosphingobium aromaticivorans DSM 12444 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 36000, recombinant enzyme, SDS-PAGE Sphingobium sp.
monomer 1 * 36837, calculated from amino acid sequence Sphingobium sp.

Synonyms

Synonyms Comment Organism
pinoresinol/lariciresinol reductase
-
Novosphingobium aromaticivorans
pinoresinol/lariciresinol reductase
-
Sphingobium sp.
PinZ
-
Novosphingobium aromaticivorans
PinZ
-
Sphingobium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Novosphingobium aromaticivorans
45
-
-
Sphingobium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5
-
Sphingobium sp.
7.5 8.5
-
Novosphingobium aromaticivorans

Cofactor

Cofactor Comment Organism Structure
NADPH activity is 15fold higher with NADPH than that in the presence of NADH, suggesting that NADPH is a preferred electron donor Novosphingobium aromaticivorans
NADPH activity is 7fold higher with NADPH than that in the presence of NADH, suggesting that NADPH is a preferred electron donor Sphingobium sp.