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Literature summary for 1.21.99.5 extracted from
- Purification, cloning, and sequencing of an enzyme mediating the reductive dechlorination of tetrachloroethylene (PCE) from Clostridium bifermentans DPH-1 (2001), Can. J. Microbiol., 47, 448-456.
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | can play important role in breakdown of perchloroethene and other chlorinated aliphatic compounds in sites contaminated with mixtures of halogenated substances | Paraclostridium bifermentans |
| agriculture | bioremediation of chloroethylenes, multiple chlorinated aliphatic molecules, in contaminated sites and water, anaerobic reductive dehalogenation of toxic xenobiotic | Paraclostridium bifermentans |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Paraclostridium bifermentans |
General Stability
| General Stability | Organism |
|---|---|
| enzyme generally unstable | Paraclostridium bifermentans |
| enzyme oxygen sensitive | Paraclostridium bifermentans |
| purified enzyme not stable, 50% loss of activity at -30°C | Paraclostridium bifermentans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | no effect, no inhibition | Paraclostridium bifermentans |  |
| Propyl iodide | 1-iodopropane; 90% inhibition by mixture of propyl iodide and titanium citrate in the dark, light restores about 95% of initial activity; light-reversible inactivation in the presence of titanium(III)citrate as electron donor | Paraclostridium bifermentans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | enzyme kinetics and kinetic parameters | Paraclostridium bifermentans | |
| 0.012 | - |
tetrachloroethene | - |
Paraclostridium bifermentans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasmic membrane | not an integral membrane protein but a peripheral membrane protein | Paraclostridium bifermentans | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cobalamin | cobalamin-containing enzyme | Paraclostridium bifermentans | |
| Cobalamin | no significant effect of cyanocobalamin | Paraclostridium bifermentans | |
| Cobalt | Co2+ corrinoid | Paraclostridium bifermentans | |
| additional information | independent of metal ions | Paraclostridium bifermentans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
2 * 35000, homodimer, SDS-PAGE | Paraclostridium bifermentans |
| 35700 | - |
2 * 35700, homodimer, MALDI-TOF/MS | Paraclostridium bifermentans |
| 70000 | - |
gel filtration | Paraclostridium bifermentans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tetrachloroethene + reduced acceptor | Paraclostridium bifermentans | - |
trichloroethene + chloride + acceptor | - |
? | |
| tetrachloroethene + reduced acceptor | Paraclostridium bifermentans DPH-1 | - |
trichloroethene + chloride + acceptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paraclostridium bifermentans | - |
DPH-1 | - |
| Paraclostridium bifermentans DPH-1 | - |
DPH-1 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | - |
Paraclostridium bifermentans |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Paraclostridium bifermentans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.086 | - |
- |
Paraclostridium bifermentans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -30°C, purified enzyme not stable, 50% loss of activity | Paraclostridium bifermentans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,1,2-trichloroethane + reduced acceptor | - |
Paraclostridium bifermentans | ? + chloride + acceptor | - |
? | |
| 1,1-dichloroethylene + reduced acceptor | - |
Paraclostridium bifermentans | ? + chloride + acceptor | - |
? | |
| 1,2-dichloropropane + reduced acceptor | - |
Paraclostridium bifermentans | ? + chloride + acceptor | - |
? | |
| cis-1,2-dichloroethylene + reduced acceptor | - |
Paraclostridium bifermentans | ? + chloride + acceptor | - |
? | |
| additional information | substrate specificity | Paraclostridium bifermentans | ? | - |
? | |
| additional information | broad substrate specificity, degrades various chlorinated aliphatic compounds | Paraclostridium bifermentans | ? | - |
? | |
| additional information | substrate specificity | Paraclostridium bifermentans DPH-1 | ? | - |
? | |
| additional information | broad substrate specificity, degrades various chlorinated aliphatic compounds | Paraclostridium bifermentans DPH-1 | ? | - |
? | |
| tetrachloroethene + reduced acceptor | - |
Paraclostridium bifermentans | trichloroethene + chloride + acceptor | - |
? | |
| tetrachloroethene + reduced acceptor | perchloroethylene, perchloroethene, PCE | Paraclostridium bifermentans | trichloroethene + chloride + acceptor | - |
? | |
| tetrachloroethene + reduced acceptor | - |
Paraclostridium bifermentans DPH-1 | trichloroethene + chloride + acceptor | - |
? | |
| tetrachloroethene + reduced acceptor | perchloroethylene, perchloroethene, PCE | Paraclostridium bifermentans DPH-1 | trichloroethene + chloride + acceptor | - |
? | |
| trans-1,2-dichloroethylene + reduced acceptor | - |
Paraclostridium bifermentans | ? + chloride + acceptor | - |
? | |
| trichloroethene + reduced acceptor | - |
Paraclostridium bifermentans | cis-1,2-dichloroethene + chloride + acceptor | - |
? | |
| trichloroethene + reduced acceptor | - |
Paraclostridium bifermentans DPH-1 | cis-1,2-dichloroethene + chloride + acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 35000, homodimer, SDS-PAGE | Paraclostridium bifermentans |
| dimer | 2 * 35700, homodimer, MALDI-TOF/MS | Paraclostridium bifermentans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Paraclostridium bifermentans |
| 35 | - |
- |
Paraclostridium bifermentans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 35 | relatively stable between | Paraclostridium bifermentans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Paraclostridium bifermentans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | most stable between | Paraclostridium bifermentans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| corrinoid | Co2+ corrinoid | Paraclostridium bifermentans | |
| additional information | no effect by NADH | Paraclostridium bifermentans |
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