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Literature summary for 1.21.99.5 extracted from

  • Neumann, A.; Wohlfarth, G.; Diekert, G.
    Properties of tetrachloroethene and trichloroethene dehalogenase of Dehalospirillum multivorans (1995), Arch. Microbiol., 163, 276-281.
No PubMed abstract available
Search for more literature for 1.21.99.5

Application

Application Comment Organism
agriculture uesd to decontaminate environment polluntant, tetrachloroethene, by tetrachloroethene bioremediation Sulfurospirillum multivorans

General Stability

General Stability Organism
enzyme insensitive towards oxygen Sulfurospirillum multivorans

Inhibitors

Inhibitors Comment Organism Structure
cyanide 0.1 mM CN-: about 50% inhibition Sulfurospirillum multivorans
EDTA 1 mM: 50% inhibition Sulfurospirillum multivorans
KCN
-
 Sulfurospirillum multivorans
additional information no inhibition by carbon monoxide, azide, hydroxylamine, nitrate, cis-1,2-dichloroethene, trans-1,2-dichloroethene or 1,1-dichloroethene Sulfurospirillum multivorans
N2O dinitrogen oxide; N2O, 100%, present in the gas phase: complete inhibition Sulfurospirillum multivorans
nitrite strong inhibition, 1 mM NO2-: complete inhibition Sulfurospirillum multivorans
Propyl iodide 1-iodopropane; 1-iodopropane, photoreversible inactivation; light-reversible inactivation in the presence of titanium(III)citrate as electron donor Sulfurospirillum multivorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information enzyme kinetics and kinetic parameters Sulfurospirillum multivorans

Metals/Ions

Metals/Ions Comment Organism Structure
Cobalt Co(III) Sulfurospirillum multivorans
additional information indicates the presence of a second transition metal Sulfurospirillum multivorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tetrachloroethene + reduced acceptor Sulfurospirillum multivorans
-
 trichloroethene + chloride + acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Sulfurospirillum multivorans
-
 strictly anaerobic, gram-negative bacterium
-
Sulfurospirillum multivorans
-
 tetrachloroethene-utilizing anaerobic bacterium
-

Reaction

Reaction Comment Organism Reaction ID
trichloroethene + chloride + acceptor = tetrachloroethene + reduced acceptor mechanism Sulfurospirillum multivorans
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tetrachloroethene + reduced acceptor
-
 Sulfurospirillum multivorans trichloroethene + chloride + acceptor
-
 ?
tetrachloroethene + reduced acceptor artificial electron donor: reduced methyl viologen, physiological electron donor is not known Sulfurospirillum multivorans trichloroethene + chloride + acceptor
-
 ?
tetrachloroethene + reduced acceptor perchloroethylene, perchloroethene, PCE Sulfurospirillum multivorans trichloroethene + chloride + acceptor
-
 ?
trichloroethene + reduced acceptor artificial electron donor: reduced methyl viologen, physiological electron donor is not known Sulfurospirillum multivorans cis-1,2-dichloroethene + chloride + acceptor
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Sulfurospirillum multivorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Sulfurospirillum multivorans

Cofactor

Cofactor Comment Organism Structure
corrinoid vitamin B12 Sulfurospirillum multivorans
vitamin B12
-
 Sulfurospirillum multivorans