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Literature summary for 1.21.99.5 extracted from
- Purification and characterization of tetrachloroethene reductive dehalogenase from Dehalospirillum multivorans (1996), J. Biol. Chem., 271, 16515-16519.
General Stability
| General Stability | Organism |
|---|---|
| enzyme slightly oxygen-sensitive | Sulfurospirillum multivorans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CCl4 | 0.1 mM: 50% inhibition | Sulfurospirillum multivorans |  |
| CH2Cl2 | 0.05 mM: 50% inhibition | Sulfurospirillum multivorans | |
| CH3Cl | 0.0008 mM: 50% inhibition | Sulfurospirillum multivorans | |
| CHCl3 | 0.025 mM: 50% inhibition | Sulfurospirillum multivorans | |
| cis-1,2-dichloroethene | slight inhibition at high concentrations, 14 mM: 50% inhibition | Sulfurospirillum multivorans | |
| cyanide | - |
Sulfurospirillum multivorans | |
| additional information | no inhibition by 1,1-dichloroethene, trans-1,2-dichloroethene, 1,1,1-trichloroethane, 1,1,1-trichloroacetone, hexachloroethane, and the chlorinated aromatic compounds 3-chlorobenzoate, 4-chlorophenol, and 3,4-dichlorobenzoate | Sulfurospirillum multivorans | |
| Propyl iodide | 1-iodopropane; light-reversible inactivation in the presence of titanium(III)citrate as electron donor | Sulfurospirillum multivorans | |
| tetrachloroethene | substrate inhibition, Ki: 18 mM | Sulfurospirillum multivorans | |
| trichloroethene | substrate inhibition, Ki: 39 mM | Sulfurospirillum multivorans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | enzyme kinetics and kinetic parameters | Sulfurospirillum multivorans | |
| 0.2 | - |
tetrachloroethene | - |
Sulfurospirillum multivorans | |
| 0.24 | - |
trichloroethene | - |
Sulfurospirillum multivorans | |
| 0.28 | - |
methyl viologen | at a tetrachloroethene concentration of 1 mM | Sulfurospirillum multivorans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | studies on cellular localization indicates presence of a cytoplasmic and a membrane-bound, periplasmic PCE dehalogenase | Sulfurospirillum multivorans | 5737 | - |
| cytoplasmic membrane | studies on cellular localization indicates presence of a cytoplasmic and a membrane-bound, periplasmic PCE dehalogenase | Sulfurospirillum multivorans | - |
- |
| cytoplasmic membrane | cytoplasmic membrane | Sulfurospirillum multivorans | - |
- |
| cytoplasmic membrane | membrane-associated, periplasmic hydrogenase | Sulfurospirillum multivorans | - |
- |
| additional information | cellular localization | Sulfurospirillum multivorans | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cobalamin | cob(II)alamin | Sulfurospirillum multivorans | |
| Cobalt | cobalt content: 1.0 mol of cobalt/mol of protein | Sulfurospirillum multivorans | |
| Cobalt | cobalt of the cofactor vitamin B12 appears to be subjected to a change in its redox state in the course of the halogenation | Sulfurospirillum multivorans | |
| Cobalt | probably present in the 2+ oxidation state | Sulfurospirillum multivorans | |
| Fe2+ | two Fe4-S4 clusters | Sulfurospirillum multivorans | |
| Fe2+ | one mol of dehalogenase contains 9.8 mol of iron, and 8.0 mol of acid-labile sulfur | Sulfurospirillum multivorans | |
| Iron-sulfur cluster | iron-sulfur clusters are present as additional cofactors, one mol of dehalogenase contains 9.8 mol of iron, and 8.0 mol of acid-labile sulfur | Sulfurospirillum multivorans | |
| Iron-sulfur cluster | two Fe4-S4 clusters | Sulfurospirillum multivorans | |
| iron-sulfur clusters | iron-sulfur clusters are present as additional cofactors, one mol of dehalogenase contains 9.8 mol of iron, and 8.0 mol of acid-labile sulfur | Sulfurospirillum multivorans | |
| iron-sulfur clusters | two Fe4-S4 clusters | Sulfurospirillum multivorans | |
| NH4+ | activation of dechlorination of perchloroethene | Sulfurospirillum multivorans | |
| NH4Cl | activation of dechlorination of perchloroethene, 4 mM: 2fold activation | Sulfurospirillum multivorans | |
| S | two Fe4-S4 clusters | Sulfurospirillum multivorans | |
| S | one mol of dehalogenase contains 9.8 mol of iron, and 8.0 mol of acid-labile sulfur | Sulfurospirillum multivorans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
1 * 57000, SDS-PAGE | Sulfurospirillum multivorans |
| 58000 | - |
gel filtration | Sulfurospirillum multivorans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| tetrachloroethene + reduced acceptor | Sulfurospirillum multivorans | PCE, physiological electron donor is not known | trichloroethene + chloride + acceptor | - |
? | |
| trichloroethene + reduced acceptor | Sulfurospirillum multivorans | physiological electron donor is not known | cis-1,2-dichloroethene + chloride + acceptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfurospirillum multivorans | - |
strictly anaerobic, gram-negative bacterium | - |
| Sulfurospirillum multivorans | - |
tetrachloroethene-utilizing anaerobic bacterium | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sulfurospirillum multivorans |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| trichloroethene + chloride + acceptor = tetrachloroethene + reduced acceptor | mechanism | Sulfurospirillum multivorans | |
| trichloroethene + chloride + acceptor = tetrachloroethene + reduced acceptor | thermodynamics | Sulfurospirillum multivorans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Sulfurospirillum multivorans |
| 158.4 | - |
- |
Sulfurospirillum multivorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | substrate specificity | Sulfurospirillum multivorans | ? | - |
? | |
| additional information | physiological electron donor is not known | Sulfurospirillum multivorans | ? | - |
? | |
| additional information | no activity with other chloroethenes, chloroethanes or chloromethanes, no activity with 1,1-dichloroethene, trans-1,2-dichloroethene, 1,1,1-trichloroethane, 1,1,1-trichloroacetone, hexachloroethane, and the chlorinated aromatic compounds 3-chlorobenzoate, 4-chlorophenol, and 3,4-dichlorobenzoate | Sulfurospirillum multivorans | ? | - |
? | |
| tetrachloroethene + reduced acceptor | artificial electron donor: reduced methyl viologen, physiological electron donor is not known | Sulfurospirillum multivorans | trichloroethene + chloride + acceptor | - |
? | |
| tetrachloroethene + reduced acceptor | perchloroethylene, perchloroethene, PCE | Sulfurospirillum multivorans | trichloroethene + chloride + acceptor | - |
? | |
| tetrachloroethene + reduced acceptor | PCE, physiological electron donor is not known | Sulfurospirillum multivorans | trichloroethene + chloride + acceptor | - |
? | |
| tetraiodoethene + reduced acceptor | reaction rate is considerably slower than with tetrachloroethene | Sulfurospirillum multivorans | triiodoethene + iodide + acceptor | - |
? | |
| trichloroethene + reduced acceptor | artificial electron donor: reduced methyl viologen, physiological electron donor is not known | Sulfurospirillum multivorans | cis-1,2-dichloroethene + chloride + acceptor | - |
? | |
| trichloroethene + reduced acceptor | physiological electron donor is not known | Sulfurospirillum multivorans | cis-1,2-dichloroethene + chloride + acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 57000, SDS-PAGE | Sulfurospirillum multivorans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Sulfurospirillum multivorans |
| 42 | - |
- |
Sulfurospirillum multivorans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
thermolabile above 50°C | Sulfurospirillum multivorans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Sulfurospirillum multivorans |
| 8 | - |
pH-optimum about 8.0 | Sulfurospirillum multivorans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| corrinoid | one mol of dehalogenase contains 1.0 mol of corrinoid | Sulfurospirillum multivorans | |
| corrinoid | vitamin B12 | Sulfurospirillum multivorans | |
| corrinoid | dicyano-cobalamin | Sulfurospirillum multivorans | |
| corrinoid | presence of a corrinoid in the cob(II)alamin form | Sulfurospirillum multivorans | |
| vitamin B12 | - |
Sulfurospirillum multivorans | |
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