Purification and characterization of tetrachloroethene reductive dehalogenase from Dehalospirillum multivorans
Neumann, A.; Wohlfarth, G.; Diekert, G.; J. Biol. Chem. 271, 16515-16519 (1996) 
Data extracted from this reference:
General Stability
General Stability
Organism
enzyme slightly oxygen-sensitive
Sulfurospirillum multivorans
Inhibitors
Inhibitors
Commentary
Organism
Structure
CCl4
0.1 mM: 50% inhibition
Sulfurospirillum multivorans
CH2Cl2
0.05 mM: 50% inhibition
Sulfurospirillum multivorans
CH3Cl
0.0008 mM: 50% inhibition
Sulfurospirillum multivorans
CHCl3
0.025 mM: 50% inhibition
Sulfurospirillum multivorans
cis-1,2-dichloroethene
slight inhibition at high concentrations, 14 mM: 50% inhibition
Sulfurospirillum multivorans
cyanide
-
Sulfurospirillum multivorans
additional information
no inhibition by 1,1-dichloroethene, trans-1,2-dichloroethene, 1,1,1-trichloroethane, 1,1,1-trichloroacetone, hexachloroethane, and the chlorinated aromatic compounds 3-chlorobenzoate, 4-chlorophenol, and 3,4-dichlorobenzoate
Sulfurospirillum multivorans
Propyl iodide
1-iodopropane; light-reversible inactivation in the presence of titanium(III)citrate as electron donor
Sulfurospirillum multivorans
tetrachloroethene
substrate inhibition, Ki: 18 mM
Sulfurospirillum multivorans
trichloroethene
substrate inhibition, Ki: 39 mM
Sulfurospirillum multivorans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
enzyme kinetics and kinetic parameters
Sulfurospirillum multivorans
0.2
-
tetrachloroethene
-
Sulfurospirillum multivorans
0.24
-
trichloroethene
-
Sulfurospirillum multivorans
0.28
-
methyl viologen
at a tetrachloroethene concentration of 1 mM
Sulfurospirillum multivorans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
studies on cellular localization indicates presence of a cytoplasmic and a membrane-bound, periplasmic PCE dehalogenase
Sulfurospirillum multivorans
5737
-
cytoplasmic membrane
studies on cellular localization indicates presence of a cytoplasmic and a membrane-bound, periplasmic PCE dehalogenase
Sulfurospirillum multivorans
-
-
cytoplasmic membrane
cytoplasmic membrane
Sulfurospirillum multivorans
-
-
cytoplasmic membrane
membrane-associated, periplasmic hydrogenase
Sulfurospirillum multivorans
-
-
additional information
cellular localization
Sulfurospirillum multivorans
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cobalamin
cob(II)alamin
Sulfurospirillum multivorans
Cobalt
cobalt content: 1.0 mol of cobalt/mol of protein
Sulfurospirillum multivorans
Cobalt
cobalt of the cofactor vitamin B12 appears to be subjected to a change in its redox state in the course of the halogenation
Sulfurospirillum multivorans
Cobalt
probably present in the 2+ oxidation state
Sulfurospirillum multivorans
Fe2+
two Fe4-S4 clusters
Sulfurospirillum multivorans
Fe2+
one mol of dehalogenase contains 9.8 mol of iron, and 8.0 mol of acid-labile sulfur
Sulfurospirillum multivorans
Iron-sulfur cluster
iron-sulfur clusters are present as additional cofactors, one mol of dehalogenase contains 9.8 mol of iron, and 8.0 mol of acid-labile sulfur
Sulfurospirillum multivorans
Iron-sulfur cluster
two Fe4-S4 clusters
Sulfurospirillum multivorans
iron-sulfur clusters
iron-sulfur clusters are present as additional cofactors, one mol of dehalogenase contains 9.8 mol of iron, and 8.0 mol of acid-labile sulfur
Sulfurospirillum multivorans
iron-sulfur clusters
two Fe4-S4 clusters
Sulfurospirillum multivorans
NH4+
activation of dechlorination of perchloroethene
Sulfurospirillum multivorans
NH4Cl
activation of dechlorination of perchloroethene, 4 mM: 2fold activation
Sulfurospirillum multivorans
S
two Fe4-S4 clusters
Sulfurospirillum multivorans
S
one mol of dehalogenase contains 9.8 mol of iron, and 8.0 mol of acid-labile sulfur
Sulfurospirillum multivorans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
57000
-
1 * 57000, SDS-PAGE
Sulfurospirillum multivorans
58000
-
gel filtration
Sulfurospirillum multivorans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
tetrachloroethene + reduced acceptor
Sulfurospirillum multivorans
PCE, physiological electron donor is not known
trichloroethene + chloride + acceptor
-
-
?
trichloroethene + reduced acceptor
Sulfurospirillum multivorans
physiological electron donor is not known
cis-1,2-dichloroethene + chloride + acceptor
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Sulfurospirillum multivorans
-
strictly anaerobic, gram-negative bacterium
-
Sulfurospirillum multivorans
-
tetrachloroethene-utilizing anaerobic bacterium
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Sulfurospirillum multivorans
Reaction
Reaction
Commentary
Organism
Reaction ID
trichloroethene + chloride + acceptor = tetrachloroethene + reduced acceptor
mechanism
Sulfurospirillum multivorans
trichloroethene + chloride + acceptor = tetrachloroethene + reduced acceptor
thermodynamics
Sulfurospirillum multivorans
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Sulfurospirillum multivorans
158.4
-
-
Sulfurospirillum multivorans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
substrate specificity
349700
Sulfurospirillum multivorans
?
-
-
-
?
additional information
physiological electron donor is not known
349700
Sulfurospirillum multivorans
?
-
-
-
?
additional information
no activity with other chloroethenes, chloroethanes or chloromethanes, no activity with 1,1-dichloroethene, trans-1,2-dichloroethene, 1,1,1-trichloroethane, 1,1,1-trichloroacetone, hexachloroethane, and the chlorinated aromatic compounds 3-chlorobenzoate, 4-chlorophenol, and 3,4-dichlorobenzoate
349700
Sulfurospirillum multivorans
?
-
-
-
?
tetrachloroethene + reduced acceptor
artificial electron donor: reduced methyl viologen, physiological electron donor is not known
349700
Sulfurospirillum multivorans
trichloroethene + chloride + acceptor
-
-
-
?
tetrachloroethene + reduced acceptor
perchloroethylene, perchloroethene, PCE
349700
Sulfurospirillum multivorans
trichloroethene + chloride + acceptor
-
-
-
?
tetrachloroethene + reduced acceptor
PCE, physiological electron donor is not known
349700
Sulfurospirillum multivorans
trichloroethene + chloride + acceptor
-
-
-
?
tetraiodoethene + reduced acceptor
reaction rate is considerably slower than with tetrachloroethene
349700
Sulfurospirillum multivorans
triiodoethene + iodide + acceptor
-
-
-
?
trichloroethene + reduced acceptor
artificial electron donor: reduced methyl viologen, physiological electron donor is not known
349700
Sulfurospirillum multivorans
cis-1,2-dichloroethene + chloride + acceptor
-
-
-
?
trichloroethene + reduced acceptor
physiological electron donor is not known
349700
Sulfurospirillum multivorans
cis-1,2-dichloroethene + chloride + acceptor
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 57000, SDS-PAGE
Sulfurospirillum multivorans
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Sulfurospirillum multivorans
42
-
-
Sulfurospirillum multivorans
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
thermolabile above 50°C
Sulfurospirillum multivorans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Sulfurospirillum multivorans
8
-
pH-optimum about 8.0
Sulfurospirillum multivorans
Cofactor
Cofactor
Commentary
Organism
Structure
corrinoid
one mol of dehalogenase contains 1.0 mol of corrinoid
Sulfurospirillum multivorans
corrinoid
vitamin B12
Sulfurospirillum multivorans
corrinoid
dicyano-cobalamin
Sulfurospirillum multivorans
corrinoid
presence of a corrinoid in the cob(II)alamin form
Sulfurospirillum multivorans
vitamin B12
-
Sulfurospirillum multivorans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
corrinoid
one mol of dehalogenase contains 1.0 mol of corrinoid
Sulfurospirillum multivorans
corrinoid
vitamin B12
Sulfurospirillum multivorans
corrinoid
dicyano-cobalamin
Sulfurospirillum multivorans
corrinoid
presence of a corrinoid in the cob(II)alamin form
Sulfurospirillum multivorans
vitamin B12
-
Sulfurospirillum multivorans
General Stability (protein specific)
General Stability
Organism
enzyme slightly oxygen-sensitive
Sulfurospirillum multivorans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CCl4
0.1 mM: 50% inhibition
Sulfurospirillum multivorans
CH2Cl2
0.05 mM: 50% inhibition
Sulfurospirillum multivorans
CH3Cl
0.0008 mM: 50% inhibition
Sulfurospirillum multivorans
CHCl3
0.025 mM: 50% inhibition
Sulfurospirillum multivorans
cis-1,2-dichloroethene
slight inhibition at high concentrations, 14 mM: 50% inhibition
Sulfurospirillum multivorans
cyanide
-
Sulfurospirillum multivorans
additional information
no inhibition by 1,1-dichloroethene, trans-1,2-dichloroethene, 1,1,1-trichloroethane, 1,1,1-trichloroacetone, hexachloroethane, and the chlorinated aromatic compounds 3-chlorobenzoate, 4-chlorophenol, and 3,4-dichlorobenzoate
Sulfurospirillum multivorans
Propyl iodide
1-iodopropane
Sulfurospirillum multivorans
Propyl iodide
light-reversible inactivation in the presence of titanium(III)citrate as electron donor
Sulfurospirillum multivorans
tetrachloroethene
substrate inhibition, Ki: 18 mM
Sulfurospirillum multivorans
trichloroethene
substrate inhibition, Ki: 39 mM
Sulfurospirillum multivorans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
enzyme kinetics and kinetic parameters
Sulfurospirillum multivorans
0.2
-
tetrachloroethene
-
Sulfurospirillum multivorans
0.24
-
trichloroethene
-
Sulfurospirillum multivorans
0.28
-
methyl viologen
at a tetrachloroethene concentration of 1 mM
Sulfurospirillum multivorans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasm
studies on cellular localization indicates presence of a cytoplasmic and a membrane-bound, periplasmic PCE dehalogenase
Sulfurospirillum multivorans
5737
-
cytoplasmic membrane
studies on cellular localization indicates presence of a cytoplasmic and a membrane-bound, periplasmic PCE dehalogenase
Sulfurospirillum multivorans
-
-
cytoplasmic membrane
cytoplasmic membrane
Sulfurospirillum multivorans
-
-
cytoplasmic membrane
membrane-associated, periplasmic hydrogenase
Sulfurospirillum multivorans
-
-
additional information
cellular localization
Sulfurospirillum multivorans
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cobalamin
cob(II)alamin
Sulfurospirillum multivorans
Cobalt
cobalt content: 1.0 mol of cobalt/mol of protein
Sulfurospirillum multivorans
Cobalt
cobalt of the cofactor vitamin B12 appears to be subjected to a change in its redox state in the course of the halogenation
Sulfurospirillum multivorans
Cobalt
probably present in the 2+ oxidation state
Sulfurospirillum multivorans
Fe2+
two Fe4-S4 clusters
Sulfurospirillum multivorans
Fe2+
one mol of dehalogenase contains 9.8 mol of iron, and 8.0 mol of acid-labile sulfur
Sulfurospirillum multivorans
Iron-sulfur cluster
iron-sulfur clusters are present as additional cofactors, one mol of dehalogenase contains 9.8 mol of iron, and 8.0 mol of acid-labile sulfur
Sulfurospirillum multivorans
Iron-sulfur cluster
two Fe4-S4 clusters
Sulfurospirillum multivorans
iron-sulfur clusters
iron-sulfur clusters are present as additional cofactors, one mol of dehalogenase contains 9.8 mol of iron, and 8.0 mol of acid-labile sulfur
Sulfurospirillum multivorans
iron-sulfur clusters
two Fe4-S4 clusters
Sulfurospirillum multivorans
NH4+
activation of dechlorination of perchloroethene
Sulfurospirillum multivorans
NH4Cl
activation of dechlorination of perchloroethene, 4 mM: 2fold activation
Sulfurospirillum multivorans
S
two Fe4-S4 clusters
Sulfurospirillum multivorans
S
one mol of dehalogenase contains 9.8 mol of iron, and 8.0 mol of acid-labile sulfur
Sulfurospirillum multivorans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
57000
-
1 * 57000, SDS-PAGE
Sulfurospirillum multivorans
58000
-
gel filtration
Sulfurospirillum multivorans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
tetrachloroethene + reduced acceptor
Sulfurospirillum multivorans
PCE, physiological electron donor is not known
trichloroethene + chloride + acceptor
-
-
?
trichloroethene + reduced acceptor
Sulfurospirillum multivorans
physiological electron donor is not known
cis-1,2-dichloroethene + chloride + acceptor
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Sulfurospirillum multivorans
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Sulfurospirillum multivorans
158.4
-
-
Sulfurospirillum multivorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
substrate specificity
349700
Sulfurospirillum multivorans
?
-
-
-
?
additional information
physiological electron donor is not known
349700
Sulfurospirillum multivorans
?
-
-
-
?
additional information
no activity with other chloroethenes, chloroethanes or chloromethanes, no activity with 1,1-dichloroethene, trans-1,2-dichloroethene, 1,1,1-trichloroethane, 1,1,1-trichloroacetone, hexachloroethane, and the chlorinated aromatic compounds 3-chlorobenzoate, 4-chlorophenol, and 3,4-dichlorobenzoate
349700
Sulfurospirillum multivorans
?
-
-
-
?
tetrachloroethene + reduced acceptor
artificial electron donor: reduced methyl viologen, physiological electron donor is not known
349700
Sulfurospirillum multivorans
trichloroethene + chloride + acceptor
-
-
-
?
tetrachloroethene + reduced acceptor
perchloroethylene, perchloroethene, PCE
349700
Sulfurospirillum multivorans
trichloroethene + chloride + acceptor
-
-
-
?
tetrachloroethene + reduced acceptor
PCE, physiological electron donor is not known
349700
Sulfurospirillum multivorans
trichloroethene + chloride + acceptor
-
-
-
?
tetraiodoethene + reduced acceptor
reaction rate is considerably slower than with tetrachloroethene
349700
Sulfurospirillum multivorans
triiodoethene + iodide + acceptor
-
-
-
?
trichloroethene + reduced acceptor
artificial electron donor: reduced methyl viologen, physiological electron donor is not known
349700
Sulfurospirillum multivorans
cis-1,2-dichloroethene + chloride + acceptor
-
-
-
?
trichloroethene + reduced acceptor
physiological electron donor is not known
349700
Sulfurospirillum multivorans
cis-1,2-dichloroethene + chloride + acceptor
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 57000, SDS-PAGE
Sulfurospirillum multivorans
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Sulfurospirillum multivorans
42
-
-
Sulfurospirillum multivorans
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
50
-
thermolabile above 50°C
Sulfurospirillum multivorans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Sulfurospirillum multivorans
8
-
pH-optimum about 8.0
Sulfurospirillum multivorans
Other publictions for EC 1.21.99.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744924
Gadkari
Reductive tetrachloroethene d ...
Sulfurospirillum multivorans, Sulfurospirillum multivorans DSMZ 12446
FEMS Microbiol. Ecol.
94
fix176
2018
-
-
-
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
744925
Gadkari
Reductive tetrachloroethene d ...
Desulfitobacterium hafniense, Desulfitobacterium hafniense Y51
FEMS Microbiol. Ecol.
94
doi: 10.1093/femsec/fix176
2018
-
-
-
-
-
-
-
-
-
-
-
2
-
10
-
-
-
-
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
744130
Zhao
Microbial reductive dehalogen ...
Dehalobacter sp. THM1
Appl. Microbiol. Biotechnol.
101
5481-5492
2017
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
744468
Ismaeil
Identification of multiple de ...
Dehalococcoides sp. YN3
BioMed Res. Int.
2017
9191086
2017
-
-
-
-
-
-
-
-
-
-
-
4
-
6
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
744884
Kunze
Subtle changes in the active ...
Desulfitobacterium hafniense, Desulfitobacterium hafniense Y51
FEBS J.
284
3520-3535
2017
-
-
-
-
8
-
-
-
-
-
-
2
-
15
-
-
-
-
-
-
-
-
4
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
2
-
-
-
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