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Literature summary for 1.21.99.4 extracted from

  • Kuiper, G.G.; Klootwijk, W.; Visser, T.J.
    Substitution of cysteine for a conserved alanine residue in the catalytic center of type II iodothyronine deiodinase alters interaction with reducing cofactor (2002), Endocrinology, 143, 1190-1198.
    View publication on PubMed
Search for more literature for 1.21.99.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in COS cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
A131C similar Km-values for L-thyroxine and 3,3',5-triiodothyronine as the wild-type enzyme. Mutation improves the interaction with the reducing cofactor dithiothreitol Homo sapiens
A131S similar Km-values for L-thyroxine and 3,3',5-triiodothyronine as the wild-type enzyme. Mutation improves the interaction with the reducing cofactor dithiothreitol Homo sapiens
SeC133C mutant enzyme with a 1000fold increase in Km-value for L-thyroxine Homo sapiens
SeC133C/A131C mutant enzyme with more than 1000fold increase in Km-value for L-thyroxine Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
gold thioglucose
-
 Homo sapiens
iodoacetate
-
 Homo sapiens
propylthiouracil wild-type and mutant enzymes A131C and A131S are insensitive to propylthiouracil in presence ogf 20 mM dithiothreitol. When tested in presence of 0.2 mM dithiothreitol the Ic50 value for propylthiouracil is reduced to about 0.1 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00000101
-
 reverse triiodothyronine mutant enzyme A131S, in presence of 20 mM dithiothreitol Homo sapiens
0.0000045
-
 L-thyroxine mutant enzyme A131C, in presence of 20 mM dithiothreitol Homo sapiens
0.0000049
-
 L-thyroxine wild-type enzyme, in presence of 20 mM dithiothreitol Homo sapiens
0.0000054
-
 L-thyroxine mutant enzyme A131S, in presence of 20 mM dithiothreitol Homo sapiens
0.0000089
-
 reverse triiodothyronine wild-type enzyme, in presence of 20 mM dithiothreitol Homo sapiens
0.0000098
-
 reverse triiodothyronine mutant enzyme A131C, in presence of 20 mM dithiothreitol Homo sapiens
0.005
-
 L-thyroxine mutant enzyme SeC133C, in presence of 20 mM dithiothreitol Homo sapiens
0.0066
-
 L-thyroxine mutant enzyme SeC133C/A131C, in presence of 20 mM dithiothreitol Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Se contains selenocysteine in the active center Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q92813
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,3',5-triiodothyronine + AH2 rT3 ORD activity Homo sapiens diiodothyronine + iodide + A + H+
-
 ?
L-thyroxine + AH2 reaction with dithiothreitol Homo sapiens 3,3',5-triiodothyronine + iodide + A + H+
-
 ?
reverse triiodothyronine + AH2
-
 Homo sapiens diiodothyronine + iodide + A + H+
-
 r

Synonyms

Synonyms Comment Organism
type II iodothyronine deiodinase
-
 Homo sapiens