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Literature summary for 1.21.99.4 extracted from

  • Berry, M.J.; Kieffer, J.D.; Harney, J.W.; Larsen, P.R.
    Selenocysteine confers the biochemical properties charateristic of the type I iodothyronine deiodinase (1991), J. Biol. Chem., 266, 14155-14158.
    View publication on PubMed
Search for more literature for 1.21.99.4

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol high concentrations of cofactor increases type I activity Rattus sp.
additional information thiol cofactor reaction stimulation reduced in type I mutant with exchange of selenocysteine by cysteine Rattus sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in JEG-3 cells, transient transfection Rattus sp.

Protein Variants

Protein Variants Comment Organism
additional information exchange selenocysteine to cysteine by site-directed mutagenesis of isoenzyme type I Rattus sp.

Inhibitors

Inhibitors Comment Organism Structure
6-Propyl-2-thiouracil competes with thiol cofactor; type I mutant with exchange of selenocysteine by cystein is 300fold less sensitive Rattus sp.
additional information 3,3',5'-triiodothyronine blocks 5'-deiodination of L-thyroxine blocks 5'-deiodination of 3,3',5'-triiodothyronine Rattus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 3,3',5'-triiodothyronine type I Rattus sp.
0.25
-
 3,3',5'-triiodothyronine DTT 30 mM Rattus sp.
2.7
-
 3,3',5'-triiodothyronine type I mutant, exchange of selenocysteine by cysteine Rattus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome type I Rattus sp.
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,3',5'-triiodothyronine + AH2 Rattus sp. isoenzyme type I 3,3'-diiodothyronine + iodide + A + H+
-
 ?
3,3',5-triiodothyronine + AH2 Rattus sp. isoenzyme type II and type III 3,3'-diiodothyronine + iodide + A + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
kidney thyronine 5'-deiodinase type I Rattus sp.
-
liver thyronine 5'-deiodinase type I Rattus sp.
-
thyroid gland thyronine 5'-deiodinase type I Rattus sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,3',5'-triiodothyronine + AH2 isoenzyme type I Rattus sp. 3,3'-diiodothyronine + iodide + A + H+
-
 ?
3,3',5-triiodothyronine + AH2
-
 Rattus sp. 3,3'-diiodothyronine + iodide + A + H+
-
 ?
3,3',5-triiodothyronine + AH2 isoenzyme type II and type III Rattus sp. 3,3'-diiodothyronine + iodide + A + H+
-
 ?

Synonyms

Synonyms Comment Organism
iodothyronine 5'-deiodinase type I, selenoprotein, inducible by T3 (higher synthesis) and angiotensin II (positive chronotropic effect in myocard) Rattus sp.