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Literature summary for 1.21.99.3 extracted from

  • Schweizer, U.; Schlicker, C.; Braun, D.; K๖hrle, J.; Steegborn, C.
    Crystal structure of mammalian selenocysteine-dependent iodothyronine deiodinase suggests a peroxiredoxin-like catalytic mechanism (2014), Proc. Natl. Acad. Sci. USA, 111, 10526-10531 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.21.99.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta2 cells Mus musculus

Crystallization (Commentary)

Crystallization (Comment) Organism
using 20% (wtv) PEG 3350 and 0.2 M ammonium citrate (pH 7.0) Mus musculus

Protein Variants

Protein Variants Comment Organism
E200T inactive Mus musculus
R275A the mutant shows sharply reduced activity compared to the wild type enzyme Mus musculus
S167A the mutant shows about 90% reduced activity compared to the wild type enzyme Mus musculus
T169A inactive Mus musculus
T169S the mutant shows about 60% reduced activity compared to the wild type enzyme Mus musculus
U170A inactive Mus musculus
Y197F inactive Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus Q91ZI8
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Mus musculus

Subunits

Subunits Comment Organism
? x * 37000, C-terminally epitope-tagged enzyme, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
deiodinase 3
-
 Mus musculus
Dio3
-
 Mus musculus
type III deiodinase
-
 Mus musculus