Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acidic fibroblast growth factor | 50fold induction of isozyme type III 5-deiodinase activity in vitro | Rattus norvegicus | |
basic fibroblast growth factor | 45fold induction of isozyme type III 5-deiodinase activity in vitro | Rattus norvegicus | |
Epidermal growth factor | 25fold induction of isozyme type III 5-deiodinase activity in vitro | Rattus norvegicus | |
insulin-like growth factor I | slight induction of isozyme type III 5-deiodinase activity in vitro | Rattus norvegicus | |
platelet-derived growth factor | slight induction of isozyme type III 5-deiodinase activity in vitro | Rattus norvegicus | |
Vasopressin | slight induction of isozyme type III 5-deiodinase activity in vitro | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000225 | - |
3,3',5-triiodo-L-thyronine | pH 7.5, 37°C, isozyme type III | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,3',5-triiodo-L-thyronine + AH2 | Rattus norvegicus | no detectable activity in vivo, isozyme type III | 3,3'-diiodo-L-thyronine + iodide + A + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Sprague-Dawley rats | - |
Rattus norvegicus | - |
isozyme type III | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adipocyte | cell culture | Rattus norvegicus | - |
adipocyte | isozyme type III | Rattus norvegicus | - |
brown adipose tissue | cell culture | Rattus norvegicus | - |
brown adipose tissue | isozyme type III | Rattus norvegicus | - |
cell culture | brown adipocytes | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
effect of growth factors on isozyme type III | Rattus norvegicus |
0.000042 | - |
isozyme type III, brown adipocyte cell culture, in presence of 10% newborn calf serum, substrate 3,3',5-triiodo-L-thyronine | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,3',5-triiodo-L-thyronine + AH2 | - |
Rattus norvegicus | 3,3'-diiodo-L-thyronine + iodide + A + H+ | - |
? | |
3,3',5-triiodo-L-thyronine + AH2 | no detectable activity in vivo, isozyme type III | Rattus norvegicus | 3,3'-diiodo-L-thyronine + iodide + A + H+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rattus norvegicus |