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Literature summary for 1.21.4.5 extracted from

  • Habash, M.; Chu, B.C.; Trevors, J.T.; Lee, H.
    Mutational study of the role of N-terminal amino acid residues in tetrachlorohydroquinone reductive dehalogenase from Sphingomonas sp. UG30 (2009), Res. Microbiol., 160, 553-559 .
    View publication on PubMed
Search for more literature for 1.21.4.5

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sphingobium sp. UG30

Protein Variants

Protein Variants Comment Organism
C14A the mutant enzyme displays greater than 25fold decreased activity relative to the wild-type enzyme. The pH optimum decreases from 50°C for the wild-type enzyme to 44°C for mutant enzyme S12A Sphingobium sp. UG30
S12A the mutant enzyme displays greater than 25fold decreased activity relative to the wild-type enzyme, the mutant enzyme shows an increase in the molar ellipticity between 260 and 280 nm, relative to the wild-type enzyme. The pH optimum decreases from 50°C for the wild-type enzyme to 44°C for mutant enzyme S12A Sphingobium sp. UG30
S15A the mutant enzyme displays greater than 25fold decreased activity relative to the wild-type enzyme. The pH optimum decreases from 50°C for the wild-type enzyme to 40°C for mutant enzyme S12A Sphingobium sp. UG30
Y7F activity of the mutant enzyme is not significantly different from the wild-type enzyme Sphingobium sp. UG30
Y9F activity of the mutant enzyme is not significantly different from the wild-type enzyme Sphingobium sp. UG30

Inhibitors

Inhibitors Comment Organism Structure
2,3,5,6-tetrachlorohydroquinone mutant enzymes S15A and S12A displayed substrate inhibition above 0.035 mM Sphingobium sp. UG30

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
11.4
-
 2,3,5,6-tetrachlorohydroquinone pH 8.7, 37°, wild-type enzyme Sphingobium sp. UG30
16
-
 2,3,5,6-tetrachlorohydroquinone pH 8.7, 37°C, mutant enzyme C14A Sphingobium sp. UG30
31.1
-
 2,3,5,6-tetrachlorohydroquinone pH 8.7, 37°C, mutant enzyme S12A Sphingobium sp. UG30
103
-
 glutathione pH 8.7, 37°C, mutant enzyme S12A Sphingobium sp. UG30
188
-
 glutathione pH 8.7, 37°C, wild-type enzyme Sphingobium sp. UG30
527
-
 glutathione pH 8.7, 37°C, mutant enzyme S15A Sphingobium sp. UG30
790
-
 glutathione pH 8.7, 37°C, mutant enzyme C14A Sphingobium sp. UG30

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 SDS-PAGE Sphingobium sp. UG30

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,3,5,6-tetrachlorohydroquinone + 2 glutathione Sphingobium sp. UG30
-
 2,3,6-trichlorohydroquinone + Cl- + glutathione disulfide
-
 ?
2,3,6-trichlorohydroquinone + 2 glutathione Sphingobium sp. UG30
-
 2,6-dichlorohydroquinone + Cl- + glutathione disulfide
-
 ?

Organism

Organism UniProt Comment Textmining
Sphingobium sp. UG30 Q937X0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sphingobium sp. UG30

Storage Stability

Storage Stability Organism
-20°C, enzyme activity is retained during storage under these conditions for at least 6 weeks Sphingobium sp. UG30

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3,5,6-tetrachlorohydroquinone + 2 glutathione
-
 Sphingobium sp. UG30 2,3,6-trichlorohydroquinone + Cl- + glutathione disulfide
-
 ?
2,3,6-trichlorohydroquinone + 2 glutathione
-
 Sphingobium sp. UG30 2,6-dichlorohydroquinone + Cl- + glutathione disulfide
-
 ?

Subunits

Subunits Comment Organism
? x * 28000, SDS-PAGE Sphingobium sp. UG30

Synonyms

Synonyms Comment Organism
pcpC
-
 Sphingobium sp. UG30

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 mutant enzyme S15A Sphingobium sp. UG30
44
-
 mutant enzyme S12A and mutant enzyme C14A Sphingobium sp. UG30
50
-
 wild-type enzyme Sphingobium sp. UG30

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4
-
 2,3,5,6-tetrachlorohydroquinone pH 8.7, 37°C, mutant enzyme C14A Sphingobium sp. UG30
6.3
-
 2,3,5,6-tetrachlorohydroquinone pH 8.7, 37°C, mutant enzyme S12A Sphingobium sp. UG30
9.6
-
 2,3,5,6-tetrachlorohydroquinone pH 8.7, 37°, wild-type enzyme Sphingobium sp. UG30

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.087
-
 2,3,5,6-tetrachlorohydroquinone pH 8.7, 37°C, mutant enzyme C14A Sphingobium sp. UG30
0.204
-
 2,3,5,6-tetrachlorohydroquinone pH 8.7, 37°C, mutant enzyme S12A Sphingobium sp. UG30
0.839
-
 2,3,5,6-tetrachlorohydroquinone pH 8.7, 37°, wild-type enzyme Sphingobium sp. UG30