Cloned (Comment) | Organism |
---|---|
of the pyruvoyl group-forming proproteins grdE, expression in Escherichia coli | Acetoanaerobium sticklandii |
Protein Variants | Comment | Organism |
---|---|---|
C242A | no cleavage on the N-terminal side of a cysteine | Acetoanaerobium sticklandii |
C242S | cleavage on the N-terminal side of a cysteine under similar conditions with more extended half-times than wild-type enzyme | Acetoanaerobium sticklandii |
C242T | cleavage on the N-terminal side of a cysteine under similar conditions with more extended half-times than wild-type enzyme | Acetoanaerobium sticklandii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21162 | - |
selenoprotein B, alpha,beta, 1 * 21162 + 1 * 26295, mass spectroscopy, amino acid sequence | Acetoanaerobium sticklandii |
26295 | - |
selenoprotein B, alpha,beta, 1 * 21162 + 1 * 26295, mass spectroscopy, amino acid sequence | Acetoanaerobium sticklandii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl phosphate + NH3 + thioredoxin disulfide + H2O | Acetoanaerobium sticklandii | - |
glycine + phosphate + thioredoxin | - |
? | |
acetyl phosphate + NH3 + thioredoxin disulfide + H2O | Acetoanaerobium sticklandii DSM 519 | - |
glycine + phosphate + thioredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetoanaerobium sticklandii | P26971 | strain HF, DSM 519T from DSMZ | - |
Acetoanaerobium sticklandii DSM 519 | P26971 | strain HF, DSM 519T from DSMZ | - |
Purification (Comment) | Organism |
---|---|
of pyruvoyl group-forming protein grdE | Acetoanaerobium sticklandii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin | mechanism | Acetoanaerobium sticklandii | |
acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin | cleavage mechanism for the pyruvoyl group dependent reductase starting from cysteine | Acetoanaerobium sticklandii | |
acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin | The reaction is observed only in the direction of glycine reduction. The enzyme consists of three protein components A, B and C. Protein B contains selenocysteine and a pyruvoyl group, and is responsible for glycine binding and ammonia release. Protein A, which also contains selenocysteine, is reduced by thioredoxin, and is needed to convert the carboxymethyl group into a ketene equivalent, in turn used by protein C to produce acetyl phosphate. Only protein B distinguishes this enzyme from EC 1.21.4.3 (sarcosine reductase) and EC 1.21.4.4 (betaine reductase) | Acetoanaerobium sticklandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl phosphate + NH3 + thioredoxin disulfide + H2O | - |
Acetoanaerobium sticklandii | glycine + phosphate + thioredoxin | - |
? | |
acetyl phosphate + NH3 + thioredoxin disulfide + H2O | - |
Acetoanaerobium sticklandii DSM 519 | glycine + phosphate + thioredoxin | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | selenoprotein B, alpha,beta, 1 * 21162 + 1 * 26295, mass spectroscopy, amino acid sequence | Acetoanaerobium sticklandii |