Cloned (Comment) | Organism |
---|---|
cloning of the genes encoding D-proline reductase, 4.8 kb EcoRI fragment containing the genes prdA and prdB isolated and sequenced, prdAcodes for a 68 kDa protein, posttranslationally cleaved to the 45 kDa and 23 kDa subunits, prdB encodes the 26 kDa subunit | Acetoanaerobium sticklandii |
D-proline reductase proproteins cloned and overexpressed in Escherichia coli XL2 blue and BL21 | Acetoanaerobium sticklandii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
10 * 23000 + 10 * 26000+ 10 * 45000, SDS-PAGE, mass spectrometry | Acetoanaerobium sticklandii |
45000 | - |
10 * 23000 + 10 * 26000+ 10 * 45000, SDS-PAGE, mass spectrometry | Acetoanaerobium sticklandii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetoanaerobium sticklandii | - |
strain ATCC12662 identical with strain DSM 519 T | - |
Purification (Comment) | Organism |
---|---|
- |
Acetoanaerobium sticklandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-proline + dithiothreitol | Stickland reaction | Acetoanaerobium sticklandii | 5-amino pentanoic acid + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
decamer | 10 * 23000 + 10 * 26000+ 10 * 45000, SDS-PAGE, mass spectrometry | Acetoanaerobium sticklandii |