Cloned (Comment) | Organism |
---|---|
expressed in the methylotrophic Pichia pastoris strain SMD1168h | Cannabis sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.131 | - |
cannabigerolate | recombinant enzyme, in 100 mM sodium citrate (pH 5.5), at 30°C | Cannabis sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58600 | - |
x * 58600, mature enzyme, calculated from amino acid sequence | Cannabis sativa |
59000 | - |
x * 59000, deglycosylated enzyme, SDS-PAGE | Cannabis sativa |
79000 | - |
x * 79000, glycosylated enzyme, SDS-PAGE | Cannabis sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cannabis sativa | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme contains seven possible Asn glycosylation sites. Endoglycosidase treatment affords a deglycosylated enzyme with more catalytic activity than that of the glycosylated form | Cannabis sativa |
Purification (Comment) | Organism |
---|---|
hydroxylapatite column chromatography | Cannabis sativa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.078 | - |
unpurified recombinant enzyme, in 100 mM sodium citrate (pH 5.5), at 30°C | Cannabis sativa |
0.41 | - |
recombinant enzyme after 5250fold purification, in 100 mM sodium citrate (pH 5.5), at 30°C | Cannabis sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cannabigerolate + O2 | about 98% conversion rate in 24 h | Cannabis sativa | DELTA9-tetrahydrocannabinolate + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58600, mature enzyme, calculated from amino acid sequence | Cannabis sativa |
? | x * 59000, deglycosylated enzyme, SDS-PAGE | Cannabis sativa |
? | x * 79000, glycosylated enzyme, SDS-PAGE | Cannabis sativa |
Synonyms | Comment | Organism |
---|---|---|
DELTA1-tetrahydrocannabinolic acid synthase | - |
Cannabis sativa |
THCA synthase | - |
Cannabis sativa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.888 | - |
cannabigerolate | recombinant enzyme, in 100 mM sodium citrate (pH 5.5), at 30°C | Cannabis sativa |
Organism | Comment | Expression |
---|---|---|
Cannabis sativa | the secreted enzyme activity in Pichia pastoris cultures is increased to 472% in the presence of 0.5% (w/v) casamino acids, 277% in the presence of proteinase inhibitors (10 mg/l 4-(2-aminoethyl) benzensulfonyl fluoride, 1 mg/l pepstatin A, and 1 mg/l E-64), 226% in the presence of 10 mg/ml FAD, 248% in the presence of 10 mg/ml FMN, 323% in the presence of 10 mg/ml riboflavin, and 298% in the presence of 1% (v/v) methanol | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.78 | - |
cannabigerolate | recombinant enzyme, in 100 mM sodium citrate (pH 5.5), at 30°C | Cannabis sativa |