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Literature summary for 1.21.3.4 extracted from
- Enzymatic synthesis of (+)- and (-)-bisdechlorogeodin with sulochrin oxidase from Penicillium frequentans and Oospora sulphurea-ochracea (1982), Arch. Microbiol., 131, 208-211.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibited by 2 mM neocuproin, 5 mM, potassium cyanide or 10 mM EDTA | Penicillium glabrum | |
| Sodium diethyldithiocarbamate | 0.1 mM, 66% inhibition, 1 mM, complete inhibition | Penicillium glabrum |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0065 | - |
sulochrin | - |
Penicillium glabrum | |
| 0.063 | - |
O2 | - |
Penicillium glabrum | |
| 0.4 | - |
N,N-Dimethyl-p-phenylenediamine | - |
Penicillium glabrum | |
| 1 | - |
N,N,N',N'-tetramethyl-p-phenylenediamine | - |
Penicillium glabrum | |
| 2 | - |
p-phenylenediamine | - |
Penicillium glabrum | |
| 5 | - |
o-phenylenediamine | - |
Penicillium glabrum | |
| 40 | - |
catechol | - |
Penicillium glabrum | |
| 60 | - |
hydroquinone | - |
Penicillium glabrum | |
| 150 | - |
(+)-ascorbic acid | - |
Penicillium glabrum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | no extracellular activity | Penicillium glabrum | 5622 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| copper | enzyme contains 6 copper atoms | Penicillium glabrum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27800 | - |
1 * 85000 + 1 * 27800, possible structure: 2 noncovalently bound identical subunits, each of which consists of 3 smaller subunits, SDS-PAGE | Penicillium glabrum |
| 85000 | - |
1 * 85000 + 1 * 27800, possible structure: 2 noncovalently bound identical subunits, each of which consists of 3 smaller subunits, SDS-PAGE | Penicillium glabrum |
| 157000 | - |
gel filtration | Penicillium glabrum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sulochrin + O2 | Penicillium glabrum | main function: synthesis of bisdechlorogeodin, involved in biosynthesis of mold metabolites related to the antibiotic griseofulvin | (+)-bisdechlorogeodin + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium glabrum | - |
- |
- |
| Penicillium glabrum CMI 96659 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 19.5% carbohydrate | Penicillium glabrum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, pH 3.3, isoelectric focusing, gel filtration | Penicillium glabrum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Penicillium glabrum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.1 | - |
- |
Penicillium glabrum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, pH 5.8, citrate-phosphate buffer, several months, no loss in activity | Penicillium glabrum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (+)-ascorbic acid + O2 | - |
Penicillium glabrum | ? | - |
? | |
| catechol + O2 | - |
Penicillium glabrum | ? | - |
? | |
| catechol + O2 | - |
Penicillium glabrum CMI 96659 | ? | - |
? | |
| hydroquinone + O2 | - |
Penicillium glabrum | ? | - |
? | |
| hydroquinone + O2 | - |
Penicillium glabrum CMI 96659 | ? | - |
? | |
| N,N,N',N'-tetramethyl-p-phenylenediamine + O2 | - |
Penicillium glabrum | ? | - |
? | |
| N,N,N',N'-tetramethyl-p-phenylenediamine + O2 | - |
Penicillium glabrum CMI 96659 | ? | - |
? | |
| N,N-dimethyl-p-phenylenediamine + O2 | - |
Penicillium glabrum | ? | - |
? | |
| N,N-dimethyl-p-phenylenediamine + O2 | - |
Penicillium glabrum CMI 96659 | ? | - |
? | |
| o-phenylenediamine + O2 | - |
Penicillium glabrum | ? | - |
? | |
| p-phenylenediamine + O2 | - |
Penicillium glabrum | ? | - |
? | |
| p-phenylenediamine + O2 | - |
Penicillium glabrum CMI 96659 | ? | - |
? | |
| sulochrin + O2 | stereospecific formation of (+)-isomer | Penicillium glabrum | (+)-bisdechlorogeodin + H2O | - |
? | |
| sulochrin + O2 | main function: synthesis of bisdechlorogeodin, involved in biosynthesis of mold metabolites related to the antibiotic griseofulvin | Penicillium glabrum | (+)-bisdechlorogeodin + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 85000 + 1 * 27800, possible structure: 2 noncovalently bound identical subunits, each of which consists of 3 smaller subunits, SDS-PAGE | Penicillium glabrum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Penicillium glabrum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | - |
- |
Penicillium glabrum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 7 | - |
Penicillium glabrum |
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