Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.21.3.3 extracted from

  • Steffens, P.; Nagakura, N.; Zenk, M.H.
    Purification and characterization of the berberine bridge enzyme from Berberis beaniana cell cultures (1985), Phytochemistry, 24, 2577-2583.
No PubMed abstract available
Search for more literature for 1.21.3.3

Inhibitors

Inhibitors Comment Organism Structure
(R)-norreticuline 50% inhibition at 0.02 mM Berberis beaniana
(S)-coreximine 50% inhibition at 0.2 mM Berberis beaniana
(S)-norreticuline 50% inhibition at 0.001 mM Berberis beaniana
(S)-scoulerine
-
 Berberis beaniana
Berberine 50% inhibition at 0.004 mM Berberis beaniana
diethyldithiocarbamate 50% inhibition at 0.4 mM Berberis beaniana
H2O2 50% inhibition at 0.7 M Berberis beaniana
Jatrorrhizine 50% inhibition at 0.03 mM Berberis beaniana
Na2EDTA 50% inhibition at 6 mM Berberis beaniana
o-phenanthroline 50% inhibition at 0.006 mM Berberis beaniana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 gel filtration Berberis beaniana
54000
-
 1 * 54000, SDS-PAGE Berberis beaniana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-reticuline + O2 Embryophyta
-
 (S)-scoulerine + H2O2
-
 ?
(S)-reticuline + O2 Berberis beaniana reticuline is the biogenic precursor of the protoberine skeleton (S)-scoulerine + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Berberis beaniana
-
-
-
Embryophyta
-
 overview of occurence of BBE in Berberidaceae, Ranunculaceae, Menispermaceae, Papaveraceae and Fumariaceae
-

Purification (Commentary)

Purification (Comment) Organism
-
 Berberis beaniana

Storage Stability

Storage Stability Organism
-20°C, 320 d, pH 7.4, 50% activity Berberis beaniana
25°C, 18 d, pH 7.4, 50% activity Berberis beaniana
37°C, 6 d, pH 7.4, 50% activity Berberis beaniana
4°C, 150 d, pH 7.4, 50% activity Berberis beaniana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R,S)-laudanosoline + O2 specific for the (S)-enantiomer Berberis beaniana ? + H2O2
-
 ?
(S)-protosinomenine + O2
-
 Berberis beaniana ? + H2O2
-
 ?
(S)-reticuline + O2
-
 Embryophyta (S)-scoulerine + H2O2
-
 ?
(S)-reticuline + O2 reticuline is the biogenic precursor of the protoberine skeleton Berberis beaniana (S)-scoulerine + H2O2
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 54000, SDS-PAGE Berberis beaniana