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Literature summary for 1.21.3.10 extracted from
- Crystal structure of the ergothioneine sulfoxide synthase from Candidatus Chloracidobacterium thermophilum and structure-guided engineering to modulate its substrate selectivity (2019), ACS Catal., 9, 6955-6961 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Chloracidobacterium thermophilum |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| to 2.5 A resolution. Enzyme is a tetramer. The active site is located at the interface between the N- and C-terminal domains for each monomer where a mononuclear nonheme iron is coordinated by His62, His153, His157 and three water molecules in an octahedral arrangement | Chloracidobacterium thermophilum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A420Y | position is involved in controlling the reaction selectiviy. Mutant leads to an increased amount of side-reaction (cysteine dioxygenase activity) | Chloracidobacterium thermophilum |
| D52L | mutation may disrupt the hydrogen bond between Asp52 and glutamyl group of substrate gamma-Glu-Cys, which in turn alters the substrate selectivity | Chloracidobacterium thermophilum |
| D52L/A420Y | mutation tunes EgtB activity toward Egt1-type, i.e. reaction of EC 1.21.3.10 | Chloracidobacterium thermophilum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
hercynine | mutant D52L, pH not specified in the publication, temperature not specified in the publication | Chloracidobacterium thermophilum |  |
| 0.023 | - |
hercynine | mutant D52L/A420Y, pH not specified in the publication, temperature not specified in the publication | Chloracidobacterium thermophilum | |
| 0.028 | - |
L-cysteine | mutant A420Y, pH not specified in the publication, temperature not specified in the publication | Chloracidobacterium thermophilum | |
| 0.039 | - |
hercynine | mutant A420Y, pH not specified in the publication, temperature not specified in the publication | Chloracidobacterium thermophilum | |
| 0.047 | - |
L-cysteine | mutant D52L, pH not specified in the publication, temperature not specified in the publication | Chloracidobacterium thermophilum | |
| 0.049 | - |
L-cysteine | mutant D52L/A420Y, pH not specified in the publication, temperature not specified in the publication | Chloracidobacterium thermophilum | |
| 0.088 | - |
hercynine | pH not specified in the publication, temperature not specified in the publication | Chloracidobacterium thermophilum | |
| 0.205 | - |
L-cysteine | wild-type, pH not specified in the publication, temperature not specified in the publication | Chloracidobacterium thermophilum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | protein conatains 0.92 iron per monomer. A mononuclear nonheme iron is coordinated by His62, His153, His157 and three water molecules in an octahedral arrangement | Chloracidobacterium thermophilum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chloracidobacterium thermophilum | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hercynine + L-cysteine + O2 | - |
Chloracidobacterium thermophilum | S-(hercyn-2-yl)-L-cysteine S-oxide + H2O | - |
? | |
| additional information | enzyme additionally accepts gamma-L-glutamyl-L-cysteine, i.e. reaction of EC 1.14.99.50 | Chloracidobacterium thermophilum | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EgtB | - |
Chloracidobacterium thermophilum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.028 | - |
L-cysteine | mutant A420Y, pH not specified in the publication, temperature not specified in the publication | Chloracidobacterium thermophilum | |
| 0.047 | - |
L-cysteine | mutant D52L, pH not specified in the publication, temperature not specified in the publication | Chloracidobacterium thermophilum | |
| 0.049 | - |
L-cysteine | mutant D52L/A420Y, pH not specified in the publication, temperature not specified in the publication | Chloracidobacterium thermophilum | |
| 0.205 | - |
L-cysteine | wild-type, pH not specified in the publication, temperature not specified in the publication | Chloracidobacterium thermophilum | |
| 0.44 | - |
hercynine | wild-type, pH not specified in the publication, temperature not specified in the publication | Chloracidobacterium thermophilum | |
| 0.46 | - |
hercynine | mutant A420Y, pH not specified in the publication, temperature not specified in the publication | Chloracidobacterium thermophilum | |
| 0.46 | - |
hercynine | mutant D52L, pH not specified in the publication, temperature not specified in the publication | Chloracidobacterium thermophilum | |
| 0.55 | - |
hercynine | mutant D52L/A420Y, pH not specified in the publication, temperature not specified in the publication | Chloracidobacterium thermophilum | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | EgtB of Candidatus Chloracidobacterium thermophilum has both EgtB- and Egt1-type of activities, i.e. reactions of EC 1.14.99.50 and EC 1.21.3.10 | Chloracidobacterium thermophilum |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.2 | - |
L-cysteine | wild-type, pH not specified in the publication, temperature not specified in the publication | Chloracidobacterium thermophilum | |
| 5 | - |
hercynine | pH not specified in the publication, temperature not specified in the publication | Chloracidobacterium thermophilum | |
| 9.7 | - |
L-cysteine | mutant D52L, pH not specified in the publication, temperature not specified in the publication | Chloracidobacterium thermophilum | |
| 11.2 | - |
L-cysteine | mutant D52L/A420Y, pH not specified in the publication, temperature not specified in the publication | Chloracidobacterium thermophilum | |
| 11.7 | - |
hercynine | mutant A420Y, pH not specified in the publication, temperature not specified in the publication | Chloracidobacterium thermophilum | |
| 16.3 | - |
L-cysteine | mutant A420Y, pH not specified in the publication, temperature not specified in the publication | Chloracidobacterium thermophilum | |
| 23.3 | - |
hercynine | mutant D52L/A420Y, pH not specified in the publication, temperature not specified in the publication | Chloracidobacterium thermophilum | |
| 35 | - |
hercynine | mutant D52L, pH not specified in the publication, temperature not specified in the publication | Chloracidobacterium thermophilum | |
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