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Literature summary for 1.21.3.10 extracted from

  • Pluskal, T.; Ueno, M.; Yanagida, M.
    Genetic and metabolomic dissection of the ergothioneine and selenoneine biosynthetic pathway in the fission yeast, S. pombe, and construction of an overproduction system (2014), PLoS ONE, 9, e97774 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.21.3.10

Cloned(Commentary)

Cloned (Comment) Organism
gene egt1+, DNA and amino acid sequence determination and analysis, sequence comparisons, and phylogenetic tree Schizosaccharomyces pombe

Protein Variants

Protein Variants Comment Organism
additional information construction of an egt1+ overexpression system by replacing its native promoter with the nmt1+ promoter, which is inducible in the absence of thiamine. Generation of a egt1+ deletion mutant, DELTAegt1, by replacing the target loci in the wild-type 972 strain with the kanamycin resistance marker (kanMX) leading to absence of all ergothioneine pathway intermediates and ergothioneine itself in DELTAegt1. Employment of three versions of the nmt1 promoter plasmid with increasing strength of expression and constructed three strains P81nmt1-egt1+, P41nmt1-egt1+, and P3nmt1-egt1+, respectively. Mutant DELTAegt1 strain shows no growth defects during cultivation in either rich (YE) or minimal (EMM2) culture media, deletion of gene egt1+ causes no significant perturbation to the intracellular metabolome of quiescent cells. No sensitivity or resistance of the mutant strains to oxidative stress compared to wild-type 972 strain Schizosaccharomyces pombe

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required for catalysis Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hercynine + L-cysteine + O2 Schizosaccharomyces pombe
-
 S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?
hercynine + L-cysteine + O2 Schizosaccharomyces pombe 972
-
 S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?
additional information Schizosaccharomyces pombe the ergothioneine pathway can also synthesize selenoneine, a selenium-containing derivative of ergothioneine, when the culture medium is supplemented with selenium. Selenoneine biosynthesis, unlike ergothioneine biosynthesis, does not produce a sulfoxide as its intermediate, but produces hercynylselenocysteine instead. Enzyme Egt-1 also catalyzes the synthesis of hercynine, EC 2.1.1.44 ?
-
 ?
additional information Schizosaccharomyces pombe 972 the ergothioneine pathway can also synthesize selenoneine, a selenium-containing derivative of ergothioneine, when the culture medium is supplemented with selenium. Selenoneine biosynthesis, unlike ergothioneine biosynthesis, does not produce a sulfoxide as its intermediate, but produces hercynylselenocysteine instead. Enzyme Egt-1 also catalyzes the synthesis of hercynine, EC 2.1.1.44 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe O94632
-
-
Schizosaccharomyces pombe 972 O94632
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hercynine + L-cysteine + O2
-
 Schizosaccharomyces pombe S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?
hercynine + L-cysteine + O2
-
 Schizosaccharomyces pombe 972 S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?
additional information the ergothioneine pathway can also synthesize selenoneine, a selenium-containing derivative of ergothioneine, when the culture medium is supplemented with selenium. Selenoneine biosynthesis, unlike ergothioneine biosynthesis, does not produce a sulfoxide as its intermediate, but produces hercynylselenocysteine instead. Enzyme Egt-1 also catalyzes the synthesis of hercynine, EC 2.1.1.44 Schizosaccharomyces pombe ?
-
 ?
additional information hercynylcysteine sulfoxide can spontaneously convert into ergothioneine in the presence of pyridoxal 5'-phosphate Schizosaccharomyces pombe ?
-
 ?
additional information the ergothioneine pathway can also synthesize selenoneine, a selenium-containing derivative of ergothioneine, when the culture medium is supplemented with selenium. Selenoneine biosynthesis, unlike ergothioneine biosynthesis, does not produce a sulfoxide as its intermediate, but produces hercynylselenocysteine instead. Enzyme Egt-1 also catalyzes the synthesis of hercynine, EC 2.1.1.44 Schizosaccharomyces pombe 972 ?
-
 ?
additional information hercynylcysteine sulfoxide can spontaneously convert into ergothioneine in the presence of pyridoxal 5'-phosphate Schizosaccharomyces pombe 972 ?
-
 ?

Subunits

Subunits Comment Organism
More Egt-1 domain structure, overview Schizosaccharomyces pombe

Synonyms

Synonyms Comment Organism
Egt-1
-
 Schizosaccharomyces pombe
egt1+
-
 Schizosaccharomyces pombe
ergothioneine biosynthesis protein 1
-
 Schizosaccharomyces pombe
mug158+/SPBC1604.01 locus name Schizosaccharomyces pombe
protein SPBC1604.01
-
 Schizosaccharomyces pombe
SPBC1604.01 locus name Schizosaccharomyces pombe

General Information

General Information Comment Organism
evolution mug158+/SPBC1604.01 is a distant homologue of the mycobacterial EgtD and EgtB genes, encoding a single fusion protein. The Schizosaccharomyces pombe homologue utilizes cysteine as a substrate, rather than using gamma-glutamyl-cysteine, as in the case of the bacterial EgtB enzyme, EC 1.14.99.50 Schizosaccharomyces pombe
metabolism the enzyme produces hercynylcysteine sulfoxide in the ergothioneine pathway. The ergothioneine pathway can also synthesize selenoneine, a selenium-containing derivative of ergothioneine, when the culture medium is supplemented with selenium. Selenoneine biosynthesis involves an intermediate compound, hercynylselenocysteine Schizosaccharomyces pombe
physiological function contribution of gene egt1+ to oxidative stress response, but gene egt1+ might not be among the primary mechanisms that protect Schizosaccharomyces pombe from exogenous peroxide Schizosaccharomyces pombe