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Literature summary for 1.21.3.10 extracted from

  • Hu, W.; Song, H.; Sae Her, A.; Bak, D.W.; Naowarojna, N.; Elliott, S.J.; Qin, L.; Chen, X.; Liu, P.
    Bioinformatic and biochemical characterizations of C-S bond formation and cleavage enzymes in the fungus Neurospora crassa ergothioneine biosynthetic pathway (2014), Org. Lett., 16, 5382-5385 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.21.3.10

Application

Application Comment Organism
synthesis the ergothioneine biosynthetic pathway (EgtA-EgtE catalysis) provides an opportunity for ergothioneine production through metabolic engineering Neurospora crassa

Cloned(Commentary)

Cloned (Comment) Organism
gene egt1, Egt1 recombinant overexpression from vector pASK-IBA3+ in Escherichia coli Neurospora crassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.39
-
 hercynine pH and temperature not specified in the publication, with gamma-L-glutamyl-L-cysteine Neurospora crassa
0.436
-
 hercynine pH and temperature not specified in the publication, with L-Cys Neurospora crassa
0.603
-
 L-cysteine pH and temperature not specified in the publication Neurospora crassa
7.68
-
 gamma-L-glutamyl-L-cysteine pH and temperature not specified in the publication Neurospora crassa

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required for catalysis, the enzyme contains about 0.90 Fe per Egt1 monomer Neurospora crassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hercynine + gamma-L-glutamyl-L-cysteine + O2 Neurospora crassa reaction of EC 1.14.99.50, low activity gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?
hercynine + gamma-L-glutamyl-L-cysteine + O2 Neurospora crassa ATCC 24698 reaction of EC 1.14.99.50, low activity gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?
hercynine + L-cysteine + O2 Neurospora crassa
-
 S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?
hercynine + L-cysteine + O2 Neurospora crassa ATCC 24698
-
 S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Neurospora crassa Q7RX33
-
-
Neurospora crassa ATCC 24698 Q7RX33
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hercynine + gamma-L-glutamyl-L-cysteine + O2 reaction of EC 1.14.99.50, low activity Neurospora crassa gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?
hercynine + gamma-L-glutamyl-L-cysteine + O2 reaction of EC 1.14.99.50, low activity Neurospora crassa ATCC 24698 gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?
hercynine + L-cysteine + O2
-
 Neurospora crassa S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?
hercynine + L-cysteine + O2
-
 Neurospora crassa ATCC 24698 S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?
additional information three different assay methods for enzyme activity determination: a 1H NMR assay of chemical shift of the imidazole hydrogen atoms, an oxygen consumption assay, and a 13C NMR assay to monitor the presence of other cysteine related oxygen consumption. Egt1 from Neurospora crassa prefers cysteine as substrate compared to gamma-L-glutamyl-L-cysteine, Egt1 has a 62fold greater specificity (kobs/Km) for L-cysteine relative to gamma-Glu-Cys Neurospora crassa ?
-
 ?
additional information three different assay methods for enzyme activity determination: a 1H NMR assay of chemical shift of the imidazole hydrogen atoms, an oxygen consumption assay, and a 13C NMR assay to monitor the presence of other cysteine related oxygen consumption. Egt1 from Neurospora crassa prefers cysteine as substrate compared to gamma-L-glutamyl-L-cysteine, Egt1 has a 62fold greater specificity (kobs/Km) for L-cysteine relative to gamma-Glu-Cys Neurospora crassa ATCC 24698 ?
-
 ?

Synonyms

Synonyms Comment Organism
Egt1
-
 Neurospora crassa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.461
-
 gamma-L-glutamyl-L-cysteine pH and temperature not specified in the publication Neurospora crassa
0.461
-
 hercynine pH and temperature not specified in the publication, with gamma-L-glutamyl-L-cysteine Neurospora crassa
2.267
-
 L-cysteine pH and temperature not specified in the publication Neurospora crassa
2.267
-
 hercynine pH and temperature not specified in the publication, with L-Cys Neurospora crassa

General Information

General Information Comment Organism
malfunction Egt1 knockout in Neurospora crassa makes the mutant much more sensitive to oxidative stress compared to wild-type Neurospora crassa
metabolism the fungus Neurospora crassa has a concise ergothioneine biosynthetic pathway, its nonheme iron enzyme Egt1 makes use of cysteine instead of gamma-Glu-Cys (like EgtB, EC 1.14.99.50) as the substrate. Such a change of substrate preference eliminates the competition between ergothioneine and glutathione biosyntheses. In Neuropsora crassa ergothioneine biosynthesis, there are reactions distinct from the pathway via EgtB: the oxidative C-S bond formation and the subsequent C-S bond cleavage reaction, which result in a net transfer of the sulfur atom form cysteine to histidine imidazole side-chain Neurospora crassa