Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | reducing agent stimulates IPNS activity | Actinomyces sp. | |
DTT | reducing agent stimulates IPNS activity | Actinomyces sp. |
Application | Comment | Organism |
---|---|---|
biotechnology | production of beta-lactam antibiotics | Actinomyces sp. |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Actinomyces sp. |
Crystallization (Comment) | Organism |
---|---|
crystal structure of the enzyme reveals that the active site of IPNS is buried in a characteristic jelly-roll motif that has been found in other oxygenases | Actinomyces sp. |
Protein Variants | Comment | Organism |
---|---|---|
Q230L | mutant of IPNS shows diminished enzyme activity, residue is highly conserved among dioxygenases and proximal to the active site, is the fourth ligand for the Fe2+ atom | Actinomyces sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | inhibition of IPNS activity | Actinomyces sp. | |
Mn2+ | inhibition of IPNS activity | Actinomyces sp. | |
N-ethylmaleimide | thiol-specific inhibitor of IPNS | Actinomyces sp. | |
Zn2+ | inhibition of IPNS activity | Actinomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine | Km values for ACV exhibit for different cyclases are on the order of 0.2-0.3 mM | Actinomyces sp. | |
0.18 | - |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine | - |
Actinomyces sp. | |
3.6 | - |
phenylacetyl-L-cysteinyl-D-valine | - |
Actinomyces sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | IPNS is iron-dependent | Actinomyces sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
adipyl-L-cysteinyl-D-valine + ? | Actinomyces sp. | - |
N-(4-carboxybutyl)penicillin + 2 H2O | - |
? | |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | Actinomyces sp. | IPNSs are nonheme iron-dependent oxygenases that catalyze cyclization | isopenicillin N + 2 H2O | - |
? | |
phenylacetyl-L-cysteinyl-D-valine + O2 | Actinomyces sp. | - |
penicillin G + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Actinomyces sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration | Actinomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adipyl-L-cysteinyl-D-valine + ? | - |
Actinomyces sp. | N-(4-carboxybutyl)penicillin + 2 H2O | - |
? | |
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 | IPNSs are nonheme iron-dependent oxygenases that catalyze cyclization | Actinomyces sp. | isopenicillin N + 2 H2O | - |
? | |
phenylacetyl-L-cysteinyl-D-valine + O2 | - |
Actinomyces sp. | penicillin G + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IPN cyclase | - |
Actinomyces sp. |
IPN synthase | - |
Actinomyces sp. |
IPNS | - |
Actinomyces sp. |
isopenicillin N synthase | - |
Actinomyces sp. |