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Literature summary for 1.21.1.1 extracted from

  • Ingavat, N.; Kavran, J.M.; Sun, Z.; Rokita, S.E.
    Active site binding is not sufficient for reductive deiodination by iodotyrosine deiodinase (2017), Biochemistry, 56, 1130-1139 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.21.1.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 80 mM Tris-HCl pH 8.5, 100 mM MgCl2, 24% (w/v) PEG 4000 and 20% glycerol Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0073
-
 3-iodo-L-tyrosine pH and temperature not specified in the publication Homo sapiens
4.1
-
 2-iodophenol pH and temperature not specified in the publication Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-iodo-L-tyrosine + NADP+ + iodide Homo sapiens
-
 3,5-diiodo-L-tyrosine + NADPH + H+
-
 ?
L-tyrosine + NADP+ + iodide Homo sapiens
-
 3-iodo-L-tyrosine + NADPH + H+
-
 ?
L-tyrosine + NADP+ + iodide Homo sapiens overall reaction 3,5-diiodo-L-tyrosine + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens F4KU78
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-iodophenol + NADP+ + iodide 2-iodophenol binds very weakly to the enzyme and is dehalogenated with a catalytic efficiency that is more than 4 orders of magnitude lower than that for 3-iodo-L-tyrosine Homo sapiens ?
-
 ?
3-iodo-L-tyrosine + NADP+ + iodide
-
 Homo sapiens 3,5-diiodo-L-tyrosine + NADPH + H+
-
 ?
L-tyrosine + NADP+ + iodide
-
 Homo sapiens 3-iodo-L-tyrosine + NADPH + H+
-
 ?
L-tyrosine + NADP+ + iodide overall reaction Homo sapiens 3,5-diiodo-L-tyrosine + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
IYD
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.004
-
 2-iodophenol pH and temperature not specified in the publication Homo sapiens
0.0102
-
 3-iodo-L-tyrosine pH and temperature not specified in the publication Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Homo sapiens
NADP+
-
 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0001
-
 2-iodophenol pH and temperature not specified in the publication Homo sapiens
14
-
 3-iodo-L-tyrosine pH and temperature not specified in the publication Homo sapiens