Cloned (Comment) | Organism |
---|---|
expression of a derivative lacking codons for amino acids 233 to gain a soluble protein | Mus musculus |
Crystallization (Comment) | Organism |
---|---|
structures of soluble enzyme lacking codons for amino acids 2-33 and two co-crystals containing substrates, mono- and diiodotyrosine, alternatively, at resolutions of 2.0 A, 2.45 A, and 2.6 A, respectively. Substrate coordination induces formation of an additional helix and coil that act as an active site lid to shield the resulting substrateflavin complex from solvent. This complex is stabilized by aromatic stacking and extensive hydrogen bonding between the substrate and flavin. The carbon-iodine bond of the substrate is positioned directly over the C-4a/N-5 region of the flavin to promote electron transfer | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q9DCX8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,5-diiodo-L-tyrosine + NADPH + H+ | - |
Mus musculus | 3-iodo-L-tyrosine + NADP+ + I- | - |
? | |
3-iodo-L-tyrosine + NADPH + H+ | - |
Mus musculus | L-tyrosine + NADP+ + I- | - |
? |