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Literature summary for 1.20.4.4 extracted from

  • Roos, G.; Buts, L.; Van Belle, K.; Brosens, E.; Geerlings, P.; Loris, R.; Wyns, L.; Messens, J.
    Interplay between ion binding and catalysis in the thioredoxin-coupled arsenate reductase family (2006), J. Mol. Biol., 360, 826-838.
    View publication on PubMed
Search for more literature for 1.20.4.4

Activating Compound

Activating Compound Comment Organism Structure
additional information mechanism by which potassium and tetrahedral oxyanions affect catalysis within the Trx-coupled family of arsenate reductases Staphylococcus aureus
additional information mechanism by which potassium and tetrahedral oxyanions affect catalysis within the Trx-coupled family of arsenate reductases Bacillus subtilis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Staphylococcus aureus
expressed in Escherichia coli Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment) Organism
x-ray structures of the mutants Staphylococcus aureus
x-ray structures of the mutants Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
C10S/C15A change of the P-loop geometry Staphylococcus aureus
H62Q change of the P-loop geometry Staphylococcus aureus
H62Q/N33K site directed mutagenesis Staphylococcus aureus
H62Q/N33K/E30D/G31E site directed mutagenesis Staphylococcus aureus
K33D site directed mutagenesis Bacillus subtilis
K33N site directed mutagenesis Bacillus subtilis
additional information commonly occurring mutation of a histidine (H62), located about 6 A from the potassium-binding site in Sa_ArsC, to a glutamine uncouples the kinetic dependency on potassium. Mutations within the Trx-coupled family of arsenate reductases lead to subtly different ion-dependent kinetic features Staphylococcus aureus
additional information commonly occurring mutation of a histidine (H62), located about 6 A from the potassium-binding site in Sa_ArsC, to a glutamine uncouples the kinetic dependency on potassium. Mutations within the Trx-coupled family of arsenate reductases lead to subtly different ion-dependent kinetic features Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 arsenate wild-type Staphylococcus aureus, condition: 150 mM KCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Staphylococcus aureus
0.022
-
 arsenate wild-type Staphylococcus aureus, condition: 150 mM NaCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Staphylococcus aureus
0.047
-
 arsenate wild-type Bacillus subtilis, condition: 50 mM K2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Bacillus subtilis
0.054
-
 arsenate wild-type Bacillus subtilis, condition: 150 mM KCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Bacillus subtilis
0.058
-
 arsenate wild-type Bacillus subtilis, condition: 150 mM NaCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Bacillus subtilis
0.061
-
 arsenate wild-type Staphylococcus aureus, condition: 50 mM Na2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Staphylococcus aureus
0.064
-
 arsenate wild-type Bacillus subtilis, condition: 50 mM Na2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Bacillus subtilis
0.08
-
 arsenate H62Q mutant Staphylococcus aureus, condition: 50 mM Na2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Staphylococcus aureus
0.081
-
 arsenate wild-type Staphylococcus aureus, condition: 50 mM K2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Staphylococcus aureus
0.087
-
 arsenate H62Q mutant Staphylococcus aureus, condition: 150 mM KCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Staphylococcus aureus
0.123
-
 arsenate H62Q mutant Staphylococcus aureus, condition: 150 mM NaCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Staphylococcus aureus
0.131
-
 arsenate H62Q mutant Staphylococcus aureus, condition: 50 mM K2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P45947
-
-
Staphylococcus aureus P0A006
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Staphylococcus aureus
-
 Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arsenate + thioredoxin
-
 Staphylococcus aureus arsenite + thioredoxin disulfide + H2O
-
 ?
arsenate + thioredoxin
-
 Bacillus subtilis arsenite + thioredoxin disulfide + H2O
-
 ?

Synonyms

Synonyms Comment Organism
Bs_ArsC
-
 Bacillus subtilis
Sa_ArsC
-
 Staphylococcus aureus
thioredoxin-coupled arsenate reductase detoxification enzyme Staphylococcus aureus
thioredoxin-coupled arsenate reductase detoxification enzyme Bacillus subtilis
Trx-coupled arsenate reductase
-
 Staphylococcus aureus
Trx-coupled arsenate reductase
-
 Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.58
-
 arsenate wild-type Staphylococcus aureus, condition: 150 mM NaCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Staphylococcus aureus
0.7
-
 arsenate H62Q mutant Staphylococcus aureus, condition: 150 mM KCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Staphylococcus aureus
0.75
-
 arsenate H62Q mutant Staphylococcus aureus, condition: 150 mM NaCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Staphylococcus aureus
0.9
-
 arsenate wild-type Staphylococcus aureus, condition: 150 mM KCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Staphylococcus aureus
1.3
-
 arsenate wild-type Bacillus subtilis, condition: 150 mM NaCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Bacillus subtilis
1.6
-
 arsenate wild-type Bacillus subtilis, condition: 150 mM KCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Bacillus subtilis
1.6
-
 arsenate wild-type Bacillus subtilis, condition: 50 mM K2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Bacillus subtilis
1.98
-
 arsenate H62Q mutant Staphylococcus aureus, condition: 50 mM Na2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Staphylococcus aureus
2
-
 arsenate wild-type Bacillus subtilis, condition: 50 mM Na2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Bacillus subtilis
2.9
-
 arsenate wild-type Staphylococcus aureus, condition: 50 mM Na2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Staphylococcus aureus
3.03
-
 arsenate H62Q mutant Staphylococcus aureus, condition: 50 mM K2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Staphylococcus aureus
3.65
-
 arsenate wild-type Staphylococcus aureus, condition: 50 mM K2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Staphylococcus aureus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.1
-
 arsenate H62Q mutant Staphylococcus aureus, condition: 150 mM NaCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Staphylococcus aureus
8.1
-
 arsenate H62Q mutant Staphylococcus aureus, condition: 150 mM KCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Staphylococcus aureus
22.4
-
 arsenate wild-type Bacillus subtilis, condition: 150 mM NaCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Bacillus subtilis
23.1
-
 arsenate H62Q mutant Staphylococcus aureus, condition: 50 mM K2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Staphylococcus aureus
24.8
-
 arsenate H62Q mutant Staphylococcus aureus, condition: 50 mM Na2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Staphylococcus aureus
26.4
-
 arsenate wild-type Staphylococcus aureus, condition: 150 mM NaCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Staphylococcus aureus
29.6
-
 arsenate wild-type Bacillus subtilis, condition: 150 mM KCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Bacillus subtilis
31.3
-
 arsenate wild-type Bacillus subtilis, condition: 50 mM Na2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Bacillus subtilis
34
-
 arsenate wild-type Bacillus subtilis, condition: 50 mM K2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Bacillus subtilis
45.1
-
 arsenate wild-type Staphylococcus aureus, condition: 50 mM K2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Staphylococcus aureus
47.5
-
 arsenate wild-type Staphylococcus aureus, condition: 50 mM Na2SO4, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Staphylococcus aureus
100
-
 arsenate wild-type Staphylococcus aureus, condition: 150 mM KCl, study about the impact of potassium and the tetrahedral oxyanion sulfate on the steady-state kinetic parameters Staphylococcus aureus