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Literature summary for 1.20.4.4 extracted from

  • Nunes, C.; Bras, J.; Najmudin, S.; Moura, J.; Moura, I.; Carepo, M.
    ArsC3 from Desulfovibrio alaskensis G20, a cation and sulfate-independent highly efficient arsenate reductase (2014), J. Biol. Inorg. Chem., 19, 1277-1285.
    View publication on PubMed
Search for more literature for 1.20.4.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Oleidesulfovibrio alaskensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 arsenate pH 7.6, 37°C, presence of 150 mM KCl Oleidesulfovibrio alaskensis
0.007
-
 arsenate pH 7.6, 37°C, presence of 50 mM K2SO4 Oleidesulfovibrio alaskensis
0.008
-
 arsenate pH 7.6, 37°C, presence of 150 mM NaCl Oleidesulfovibrio alaskensis

Metals/Ions

Metals/Ions Comment Organism Structure
K+ nonspecific binding Oleidesulfovibrio alaskensis
Na+ nonspecific binding Oleidesulfovibrio alaskensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
 1 * 16010, calculated, 1 * 16000, SDS-PAGE, 1 * 16008, ESI-microTOF Oleidesulfovibrio alaskensis
16008
-
 1 * 16010, calculated, 1 * 16000, SDS-PAGE, 1 * 16008, ESI-microTOF Oleidesulfovibrio alaskensis
16010
-
 1 * 16010, calculated, 1 * 16000, SDS-PAGE, 1 * 16008, ESI-microTOF Oleidesulfovibrio alaskensis
20000
-
 gel filtration Oleidesulfovibrio alaskensis

Organism

Organism UniProt Comment Textmining
Oleidesulfovibrio alaskensis
-
-
-
Oleidesulfovibrio alaskensis G20
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arsenate + thioredoxin
-
 Oleidesulfovibrio alaskensis arsenite + thioredoxin disulfide + H2O
-
 ?
arsenate + thioredoxin
-
 Oleidesulfovibrio alaskensis G20 arsenite + thioredoxin disulfide + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 16010, calculated, 1 * 16000, SDS-PAGE, 1 * 16008, ESI-microTOF Oleidesulfovibrio alaskensis

Synonyms

Synonyms Comment Organism
ArsC3
-
 Oleidesulfovibrio alaskensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1
-
 arsenate pH 7.6, 37°C, presence of 150 mM NaCl Oleidesulfovibrio alaskensis
2.9
-
 arsenate pH 7.6, 37°C, presence of 50 mM K2SO4 Oleidesulfovibrio alaskensis
3.2
-
 arsenate pH 7.6, 37°C, presence of 150 mM KCl Oleidesulfovibrio alaskensis

Cofactor

Cofactor Comment Organism Structure
thioredoxin
-
 Oleidesulfovibrio alaskensis

General Information

General Information Comment Organism
physiological function isoform ArsC3 is functional and able to confer arsenate resistance to Escherichia coli Oleidesulfovibrio alaskensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
256
-
 arsenate pH 7.6, 37°C, presence of 150 mM NaCl Oleidesulfovibrio alaskensis
409
-
 arsenate pH 7.6, 37°C, presence of 50 mM K2SO4 Oleidesulfovibrio alaskensis
531
-
 arsenate pH 7.6, 37°C, presence of 150 mM KCl Oleidesulfovibrio alaskensis