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Literature summary for 1.20.4.4 extracted from
- A novel arsenate reductase from the bacterium Thermus thermophilus HB27: its role in arsenic detoxification (2013), Biochim. Biophys. Acta, 1834, 2071-2079.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermus thermophilus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C7S | complete loss of activity | Thermus thermophilus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.635 | - |
arsenate | pH 7.0, 60°C | Thermus thermophilus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16957 | - |
1 * 16957, calculated, 1 * 17000, SDS-PAGE | Thermus thermophilus |
| 17000 | - |
gel filtration | Thermus thermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | Q72HI5 | - |
- |
| Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | Q72HI5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arsenate + thioredoxin | - |
Thermus thermophilus | arsenite + thioredoxin disulfide + H2O | - |
? | |
| arsenate + thioredoxin | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | arsenite + thioredoxin disulfide + H2O | - |
? | |
| additional information | enzyme additionally exhibits weak phosphatase activity | Thermus thermophilus | ? | - |
? | |
| additional information | enzyme additionally exhibits weak phosphatase activity | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 16957, calculated, 1 * 17000, SDS-PAGE | Thermus thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ARS | - |
Thermus thermophilus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.75 | - |
arsenate | pH 7.0, 60°C | Thermus thermophilus | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thioredoxin | - |
Thermus thermophilus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Thermus thermophilus | calculated | - |
6.5 |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Thermus thermophilus | expression is 4fold increased in presence of arsenate in the growth medium | up |
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Release 2023.1 (January 2023)
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