Literature summary for 1.20.4.4 extracted from

Villadangos, A.F.; Van Belle, K.; Wahni, K.; Dufe, V.T.; Freitas, S.; Nur, H.; De Galan, S.; Gil, J.A.; Collet, J.F.; Mateos, L.M.; Messens, J.
Corynebacterium glutamicum survives arsenic stress with arsenate reductases coupled to two distinct redox mechanisms (2011), Mol. Microbiol., 82, 998-1014.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Corynebacterium glutamicum

Crystallization (Commentary)

Crystallization (Comment)	Organism
seleno-methionine derivative, to 2.2 A resolution	Corynebacterium glutamicum

Protein Variants

Protein Variants	Comment	Organism
additional information	in mutant lacking N-terminal 78 amino acids, the kinetic parameters are greatly reduced	Corynebacterium glutamicum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.032	-	arsenate	wild-type, pH 8.0, 37°C	Corynebacterium glutamicum
0.11	-	arsenate	N-terminally truncated mutant, pH 8.0, 37°C	Corynebacterium glutamicum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
25360	-	2 * 25360, SDS-PAGE	Corynebacterium glutamicum
60000	-	gel filtration	Corynebacterium glutamicum

Organism

Organism	UniProt	Comment	Textmining
Corynebacterium glutamicum	P0DKS5	enzyme additionally shows low phosphates activity	-
Corynebacterium glutamicum DSM 20300	P0DKS5	enzyme additionally shows low phosphates activity	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
arsenate + thioredoxin	-	Corynebacterium glutamicum	arsenite + thioredoxin disulfide + H2O	-	?
arsenate + thioredoxin	-	Corynebacterium glutamicum DSM 20300	arsenite + thioredoxin disulfide + H2O	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 25360, SDS-PAGE	Corynebacterium glutamicum
More	isoform ArsC1' is unique in bearing an N-terminal three-helical bundle that interacts with the active site of the other chain in the dimeric interface	Corynebacterium glutamicum

Synonyms

Synonyms	Comment	Organism
ArsC1'	-	Corynebacterium glutamicum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.09	-	arsenate	wild-type, pH 8.0, 37°C	Corynebacterium glutamicum
0.5	-	arsenate	N-terminally truncated mutant, pH 8.0, 37°C	Corynebacterium glutamicum

Cofactor

Cofactor	Comment	Organism	Structure
additional information	no cofactor: mycoredoxin	Corynebacterium glutamicum
thioredoxin	-	Corynebacterium glutamicum

Expression

Organism	Comment	Expression
Corynebacterium glutamicum	isooform ArsC1' is constitutively expressed at low levels using its own promoter site. It reduces arsenate to arsenite that can then induce the expression of isoforms ArsC1 and ArsC2	additional information

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.045	-	arsenate	N-terminally truncated mutant, pH 8.0, 37°C	Corynebacterium glutamicum
2.9	-	arsenate	wild-type, pH 8.0, 37°C	Corynebacterium glutamicum

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Release 2023.1 (January 2023)