Any feedback?
Literature summary for 1.20.4.4 extracted from
- Novel channel enzyme fusion proteins confer arsenate resistance (2010), J. Biol. Chem., 285, 40081-40087.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli and yeast | Salinispora tropica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 75 | - |
arsenate | pH 7.5, temperature not specified in the publication | Salinispora tropica |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
Western blot | Salinispora tropica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salinispora tropica | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arsenate + thioredoxin | - |
Salinispora tropica | arsenite + thioredoxin disulfide + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| arsenate reductase | - |
Salinispora tropica |
| Strop634 | gene name, protein comprises two domains an aquaglyceroporin-derived N-terminal channel-like part fused to a C-terminal enzyme domain with similarity to ArsC arsenate reductase, protein also shows minor phosphotyrosine phosphatase activity | Salinispora tropica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Salinispora tropica |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thioredoxin | - |
Salinispora tropica | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | heterologous expression of Strop634 or its separate arsenate reductase domain complements a yeast strain lacking arsenate reductase acr2 | Salinispora tropica |
| malfunction | Salinispora tropica Strop634 deletion strains are highly sensitive to arsenate exposure | Salinispora tropica |
| physiological function | as a dual functional protein (arsenite channel and arsenate reductase) Strop634 rescues a yeast strain that is highly sensitive to arsenate due to deletion of the ACR2 reductase and all transport proteins for arsenite, ACR3, Fps1, and the vacuolar ABC transporter Ycf1 for arsenite-thiol conjugates | Salinispora tropica |
| physiological function | confers arsenate resistance in Salinispora tropica | Salinispora tropica |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.21 | - |
arsenate | pH 7.5, temperature not specified in the publication | Salinispora tropica | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
