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Literature summary for 1.20.4.4 extracted from
- Reduction of arsenate to arsenite by the ArsC protein of the arsenic resistance operon of Staphylococcus aureus plasmid pI258 (1992), Proc. Natl. Acad. Sci. USA, 89, 9474-9478.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | stimulates ArsC-dependent arsenate reduction, but not 2-mercaptoethanol or reduced glutathione | Staphylococcus aureus subsp. aureus RN4220 |  |
| additional information | reduction of arsenate to arsenite is dependent on the presence of the arsC gene and the extent of reduction depended also upon the presence of arsB. The arsC-deletion mutant plasmids pGJ106 and pGJ107 confer no more arsenate reduction activity than does the vector plasmid pSK265. Without intact arsB (plasmids pGJ105 and pGJ109) somewhat less arsenite is found | Staphylococcus aureus subsp. aureus RN4220 | |
| thioredoxin | and dithiothreitol are needed for arsenate reduction | Staphylococcus aureus subsp. aureus RN4220 | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overproduced in Escherichia coli K38(pGP1-2) | Staphylococcus aureus subsp. aureus RN4220 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetate | sulfhydryl inhibitors N-ethylmaleimide -and iodoacetate inhibit arsenate reductase activity by 80% in crude cell-free preparations and by 90% with purified ArsC protein | Staphylococcus aureus subsp. aureus RN4220 | |
| N-ethylmaleimide | sulfhydryl inhibitors N-ethylmaleimide -and iodoacetate inhibit arsenate reductase activity by 80% in crude cell-free preparations and by 90% with purified ArsC protein | Staphylococcus aureus subsp. aureus RN4220 | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Staphylococcus aureus subsp. aureus RN4220 | 5737 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus subsp. aureus RN4220 | P0A006 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by fast protein liquid chromatography | Staphylococcus aureus subsp. aureus RN4220 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arsenate + thioredoxin | - |
Staphylococcus aureus subsp. aureus RN4220 | arsenite + thioredoxin disulfide + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ArsC | plasmid p1258 | Staphylococcus aureus subsp. aureus RN4220 |
| arsenate reductase | - |
Staphylococcus aureus subsp. aureus RN4220 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Staphylococcus aureus subsp. aureus RN4220 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
heat-stable, surviving 2 min heating at 80°C | Staphylococcus aureus subsp. aureus RN4220 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.5 | ArsC arsenate reductase activity is decreased >80% at pH 6.3 but is stable in the pH range 7.0-9.5 | Staphylococcus aureus subsp. aureus RN4220 |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.5 | ArsC arsenate reductase activity is decreased >80% at pH 6.3 but is stable in the pH range 7.0-9.5 | Staphylococcus aureus subsp. aureus RN4220 |
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