Literature summary for 1.20.4.4 extracted from

Messens, J.; Van Molle, I.; Vanhaesebrouck, P.; Limbourg, M.; Van Belle, K.; Wahni, K.; Martins, J.C.; Loris, R.; Wyns, L.
How thioredoxin can reduce a buried disulphide bond (2004), J. Mol. Biol., 339, 527-537.

Search for more literature for 1.20.4.4

Cloned(Commentary)

Cloned (Comment)	Organism
C10S/C15A/C89L mutant and C10S/C15A/C82S mutant expressed in Escherichia coli	Staphylococcus aureus

Protein Variants

Protein Variants	Comment	Organism
C10S/C15A/C82S	mutant, determination of the redox potential of the Cys82-Cys89 redox couple, thioredoxin is unable to reduce the Cys10-Cys15 disulfide in oxidized ArsC C82S	Staphylococcus aureus
C10S/C15A/C89L	mutant, determination of the redox potential of the Cys82-Cys89 redox couple	Staphylococcus aureus
additional information	ArsC triple mutants and the Trx C32S mutant formate a ArsC-TrxC32S complex	Staphylococcus aureus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.033	-	thioredoxin	-	Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
14800	-	reduced C82S mutant	Staphylococcus aureus
14810	-	reducted wild-type	Staphylococcus aureus
14820	-	reduced C89L mutant	Staphylococcus aureus

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	P0A006	-	-

Purification (Commentary)

Purification (Comment)	Organism
C10S/C15A/C89L mutant and C10S/C15A/C82S mutant purified by Ni2+-NTA Superflow	Staphylococcus aureus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
arsenate + thioredoxin	-	Staphylococcus aureus	arsenite + thioredoxin disulfide + H2O	-	?

Synonyms

Synonyms	Comment	Organism
ArsC	-	Staphylococcus aureus
pI258 arsenate reductase	-	Staphylococcus aureus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.9	-	thioredoxin	-	Staphylococcus aureus

Cofactor

Cofactor	Comment	Organism	Structure
thioredoxin	interaction between thioredoxin and the enzyme pI258 arsenate reductase. Thioredoxin converts oxidized ArsC back towards its initial reduced state	Staphylococcus aureus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
57.6	-	thioredoxin	-	Staphylococcus aureus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)