Literature summary for 1.20.4.1 extracted from

Shi, J.; Vlamis-Gardikas, A.; Aslund, F.; Holmgren, A.; Rosen, B.P.
Reactivity of glutaredoxins 1,2 and 3 from Escherichia coli shows that glutaredoxin 2 is the primary hydrogen donor to ArsC-catalazed arsenate reduction (1999), J. Biol. Chem., 274, 36039-36042.

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Activating Compound

Activating Compound	Comment	Organism	Structure
GSH	the enzyme uses GSH with glutaredoxin as electron donor	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.000003	-	glutaredoxin 2	-	Escherichia coli
0.0042	-	glutaredoxin 1	-	Escherichia coli
0.3	-	glutaredoxin 3	-	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
arsenate + reduced glutaredoxin	Escherichia coli	the enzyme is involved in bacterial arsenic resistance	arsenite + oxidized glutaredoxin	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
arsenate + glutaredoxin 1	-	Escherichia coli	arsenite + glutaredoxin 1 disulfide + H2O	-	r
arsenate + glutaredoxin 2	-	Escherichia coli	arsenite + glutaredoxin 1 disulfide + H2O	-	r
arsenate + glutaredoxin 2	-	Escherichia coli	arsenite + glutaredoxin 2 disulfide + H2O	-	r
arsenate + glutaredoxin 3	-	Escherichia coli	arsenite + glutaredoxin 1 disulfide + H2O	-	r
arsenate + glutaredoxin 3	-	Escherichia coli	arsenite + glutaredoxin 3 disulfide + H2O	-	r
arsenate + reduced glutaredoxin	the enzyme uses GSH with glutaredoxin as electron donor. Glutaredoxin 2 is the most effective hydrogen donor for the reduction of arsenate. During the catalytic cycle, ArsC forms a mixed disulfide with GSH before being reduced by glutaredoxin to regenerate the active ArsC reductase	Escherichia coli	arsenite + oxidized glutaredoxin	-	?
arsenate + reduced glutaredoxin	the enzyme is involved in bacterial arsenic resistance	Escherichia coli	arsenite + oxidized glutaredoxin	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.09	-	glutaredoxin 3	-	Escherichia coli
0.14	-	glutaredoxin 2	-	Escherichia coli
0.3	-	glutaredoxin 1	-	Escherichia coli

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Release 2023.1 (January 2023)