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Literature summary for 1.20.1.1 extracted from

  • Ranaghan, K.; Hung, J.; Bartlett, G.; Mooibroek, T.; Harvey, J.; Woolfson, D.; Van Der Donk, W.; Mulholland, A.
    A catalytic role for methionine revealed by a combination of computation and experiments on phosphite dehydrogenase (2014), Chem. Sci., 5, 2191-2199 .
No PubMed abstract available
Search for more literature for 1.20.1.1

Protein Variants

Protein Variants Comment Organism
E175A the mutation allows the enzyme to use both NAD+ and NADP+ Pseudomonas stutzeri
M53A the KM values for phosphonate and NAD+ are little different from the parent enzyme, while kcat is reduced by a factor of 50 Pseudomonas stutzeri
M53N the KM values for phosphonate and NAD+ are little different from the parent enzyme, while kcat is reduced by a factor of 200 Pseudomonas stutzeri

Inhibitors

Inhibitors Comment Organism Structure
sulfite
-
 Pseudomonas stutzeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 NAD+ mutant enzyme M53A, at pH 7.3 and 25°C Pseudomonas stutzeri
0.017
-
 phosphonate mutant enzyme M53N, at pH 7.3 and 25°C Pseudomonas stutzeri
0.022
-
 NAD+ mutant enzyme E175A, at pH 7.3 and 25°C Pseudomonas stutzeri
0.028
-
 phosphonate mutant enzyme E175A, at pH 7.3 and 25°C Pseudomonas stutzeri
0.064
-
 NAD+ mutant enzyme M53N, at pH 7.3 and 25°C Pseudomonas stutzeri
0.1
-
 phosphonate mutant enzyme M53A, at pH 7.3 and 25°C Pseudomonas stutzeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphonate + NAD+ + H2O Pseudomonas stutzeri
-
 phosphate + NADH + H+
-
 ?
phosphonate + NAD+ + H2O Pseudomonas stutzeri WM88
-
 phosphate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas stutzeri O69054
-
-
Pseudomonas stutzeri WM88 O69054
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphonate + NAD+ + H2O
-
 Pseudomonas stutzeri phosphate + NADH + H+
-
 ?
phosphonate + NAD+ + H2O
-
 Pseudomonas stutzeri WM88 phosphate + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
phosphite dehydrogenase
-
 Pseudomonas stutzeri
PTDH
-
 Pseudomonas stutzeri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.016
-
 phosphonate mutant enzyme M53N, at pH 7.3 and 25°C Pseudomonas stutzeri
0.059
-
 phosphonate mutant enzyme M53A, at pH 7.3 and 25°C Pseudomonas stutzeri
3.1
-
 phosphonate mutant enzyme E175A, at pH 7.3 and 25°C Pseudomonas stutzeri

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas stutzeri

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.59
-
 phosphonate mutant enzyme M53A, at pH 7.3 and 25°C Pseudomonas stutzeri
0.92
-
 phosphonate mutant enzyme M53N, at pH 7.3 and 25°C Pseudomonas stutzeri
110
-
 phosphonate mutant enzyme E175A, at pH 7.3 and 25°C Pseudomonas stutzeri